paperclip

#28322395   2017/03/21 To Up

A simple synthetic route to polyoxovanadate-based organic-inorganic hybrids using EEDQ as an ester coupling agent.

A reaction strategy for the post-functionalization of hexavanadate derivatives is presented herein. In this study, five polyoxovanadate-based organic-inorganic hybrids TBA2[V6O13{(OCH2)3CCOO(CH2)15CH3}2] (2), TBA2[V6O13{(OCH2)3CCOO(CH2)8CH[double bond, length as m-dash]CH2}2] (3), TBA2[V6O13{(OCH2)3CCOOCH2CF3}2] (4), TBA2[V6O13{(OCH2)3CCOO(CH2CH2O)3CH3}2] (5), and TBA2[V6O13{(OCH2)3CCOO(CH2)12OH}2]·2CH3CH2OH (6) were successfully synthesized via esterification of carboxylic acid groups-containing TBA2[V6O13{(OCH2)3CCOOH}2] (1) with five alcohols possessing different functional groups. These hybrids were characterized by single crystal X-ray diffraction, IR, ESI-MS, (1)H and (13)C-NMR spectroscopies, and elemental analysis. Moreover, the formation of large vesicles was observed in a mixed solution of compound 2 due to its surfactant-like structure.

There is about (8244) Related Products to A simple synthetic route to polyoxovanadate-based organic-inorganic hybrids using EEDQ as an ester coupling agent.

Inorganic Phosphorus, UV, Simple Surgical Table For Inorganic Phosphorous, Co EEDQ (2 Ethoxy 1 ethoxyca EEDQ (2 Ethoxy 1 ethoxyca Eosin 5 thiouredylphenylb MarkerGeneTM Fluorescent Lactacystin (Synthetic) Cholesterol synthetic CAS Insulin 1 (Rat), syntheti SYNTHETIC S ADENOSYL L HO Uroguanylin (circulating

paperclip

#28347826   2017/03/28 To Up

Changes in cerebral [(18)F]-FDG uptake induced by acute alcohol administration in a rat model of alcoholism.

Several [(18)F]-FDG positron emission tomography (PET) studies in alcoholics have consistently reported decreases in overall brain glucose metabolism at rest and following acute alcohol administration. However, changes in cerebral glucose utilization associated with the transition to addiction are not well understood and require longitudinal translational imaging studies in animal models of alcoholism. Here, we studied brain glucose uptake in alcohol drinking rats in order to provide convergent evidence to what has previously been reported in human studies. Brain glucose metabolism was measured by [(18)F]-FDG microPET imaging in different male Wistar rat groups: short-term drinking (three months), long-term drinking (twelve months) and alcohol-naïve. Global and regional cerebral glucose uptake was measured at rest and following acute alcohol administration. We showed that alcohol significantly reduced the whole-brain glucose metabolism. This effect was most pronounced in the parietal cortex and cerebellum. Alcohol-induced decreases in brain [(18)F]-FDG uptake was most apparent in alcohol-naïve rats, less intense in short-term drinkers and absent in long-term drinkers. The latter finding indicates the occurrence of tolerance to the intoxicating effects of alcohol in long-term drinking individuals. In contrast, some regions, like the ventral striatum and entorhinal cortex, showed enhanced metabolic activity, an effect that did not undergo tolerance during long-term alcohol consumption. Our findings are comparable to those described in human studies using the same methodology. We conclude that [(18)F]-FDG PET studies in rat models of alcoholism provide good translation and can be used for future longitudinal studies investigating alterations in brain function during different stages of the addiction cycle.

There is about (24018) Related Products to Changes in cerebral [(18)F]-FDG uptake induced by acute alcohol administration in a rat model of alcoholism.

4 Hydroxy 3 methoxybenzyl steroidogenic acute regul Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Model 300 V T Ultrasonic Model 150 V T Ultrasonic Mouse Epstein-Barr Virus Human Epstein-Barr Virus OxiSelect™ Cellular UV- Advanced Airway Intubatio

paperclip

#28589273   2017/06/07 To Up

Synoptic sampling and principal components analysis to identify sources of water and metals to an acid mine drainage stream.

Combining the synoptic mass balance approach with principal components analysis (PCA) can be an effective method for discretising the chemistry of inflows and source areas in watersheds where contamination is diffuse in nature and/or complicated by groundwater interactions. This paper presents a field-scale study in which synoptic sampling and PCA are employed in a mineralized watershed (Lion Creek, Colorado, USA) under low flow conditions to (i) quantify the impacts of mining activity on stream water quality; (ii) quantify the spatial pattern of constituent loading; and (iii) identify inflow sources most responsible for observed changes in stream chemistry and constituent loading. Several of the constituents investigated (Al, Cd, Cu, Fe, Mn, Zn) fail to meet chronic aquatic life standards along most of the study reach. The spatial pattern of constituent loading suggests four primary sources of contamination under low flow conditions. Three of these sources are associated with acidic (pH <3.1) seeps that enter along the left bank of Lion Creek. Investigation of inflow water (trace metal and major ion) chemistry using PCA suggests a hydraulic connection between many of the left bank inflows and mine water in the Minnesota Mine shaft located to the north-east of the river channel. In addition, water chemistry data during a rainfall-runoff event suggests the spatial pattern of constituent loading may be modified during rainfall due to dissolution of efflorescent salts or erosion of streamside tailings. These data point to the complexity of contaminant mobilisation processes and constituent loading in mining-affected watersheds but the combined synoptic sampling and PCA approach enables a conceptual model of contaminant dynamics to be developed to inform remediation.

There is about (10656) Related Products to Synoptic sampling and principal components analysis to identify sources of water and metals to an acid mine drainage stream.

Zinc powder 99.9% (metals GELRED NUCLEIC ACID GEL S GELGREEN NUCLEIC ACID GEL GELRED NUCLEIC ACID STAIN GelGreen Nucleic Acid Sta GelGreen Nucleic Acid Sta GelGreen Nucleic Acid Sta GelRed Nucleic Acid Stain GelRed Nucleic Acid Stain 8 Octanoyloxypyrene 1,3,6 8 Octadecyloxypyrene 1,3, RubyGlowTM Luminescent Ba

paperclip

#28100347   2017/01/19 To Up

[Salvianolate protects H9c2 cells from hypoxia/reoxygenation injury-induced apoptosis by attenuating mitochondrial DNA oxidative damage].

Objective: To investigate the possible mechanism related to the protective effects of salvianolate in H9c2 cells underwent hypoxia/reoxygenation (H/R)-injury. Methods: H9c2 cells were divided into four groups: control group, salvianolate group (S group), H/R group, and salvianolate+ H/R group(S+ H/R group), in which the H9c2 cells were pretreated with salvianolate before H/R-treatment.Apoptotic cells were detected by Tunel assays and AnnexinⅤ-FITC apoptosis detection kit.The intracellular ATP level, the change of mitochondrial membrane potential and the mitochondrial DNA oxidative damage were also determined in these groups. Results: (1) The apoptosis rate of H/R group(26.36±5.14)% was significantly higher compared to control group(2.71±1.66)%(P=0.000 4), which could be significantly reduced in S+ H/R group(17.28±4.75)%(P=0.012 8 vs. H/R group , P=0.003 9 vs. control group). The ratio of AnnexinⅤ and PI double positive cells in H/R group(28.23±6.73)% was significantly higher compared to control group(3.53±2.83)%(P=0.001 1), which was significantly reduced in S+ H/R group(18.10±4.56)%(P=0.037 2 vs. H/R group, P=0.038 3 vs. control group). (2)The ATP level of H9c2 cells in H/R group(49.05±10.12)% was significantly lower than in control group 100%(P=0.000 5), which was significantly increased in S+ H/R group(68.67±13.32)%(P=0.019 9 vs. H/R group). Confocal microscope showed that red fluorescence was dominant in the control group, red fluorescence was significantly reduced, while green fluorescence was significantly increased in H9c2 cells of H/R group and the fluorescence ratio of red to green in H/R group((37.13±8.47)%) was significantly decreased compared to control group (100%, P=0.000 1), fluorescence ratio of red to green was significantly increased in S+ H/R group((63.77±12.32)% vs. H/R group, P=0.007 3). (3)The mitochondrial DNA oxidative damage in different groups: there was only few 8-hydroxyguanine (8-OHdG) expression, which marked as green, in control group, and 8-OHdG expression was significantly upregulated in H/R group, moreover, the 8-OHdG was co-localized with mitochondria.The expression of 8-OHdG was significantly lower in S+ H/R group compared to H/R group. Conclusion: Salvianolate can reduce mitochondrial DNA oxidative damage, and protect mitochondrial function, thus inhibit myocardial cell apoptosis and eventually reduce the myocardial H/R-injury in H9c2 cells.

There is about (8386) Related Products to [Salvianolate protects H9c2 cells from hypoxia/reoxygenation injury-induced apoptosis by attenuating mitochondrial DNA oxidative damage].

OxiSelect™ Cellular UV- Human Kidney injury molec DNA Damage, 8 OH dG EIA k DNA Damage, 8 OH dG EIA k DNA RNA Damage Apoptosis antibody array 8 Isoprostane oxidative s OXI TEK (Oxidative Stress anti CD45 RA B cells, T c Transcription factors: O Apoptosis Phospho-Specifi Cancer Apoptosis Phospho-

paperclip

#27311936   2016/07/19 To Up

In situ cultured preantral follicles is a useful model to evaluate the effect of anticancer drugs on caprine folliculogenesis.

Despite the increase in the incidence of cancer, the number of women who survive cancer treatment is growing. However, one of the principal results of chemotherapy is premature ovarian failure (POF). The aim of this study was to use the in situ culture preantral follicles as an in vitro model to evaluate the toxicity of two anticancer drugs, doxorubicin (DXR) and paclitaxel (PTX), on the integrity and development of ovarian follicles. Fragments of the ovarian cortex of goats were cultured in vitro for 1 or 7 days in α-MEM(+) supplemented with different concentrations of DXR (0.003, 0.03, or 0.3 µg/mL) and PTX (0.001, 0.01, or 0.1 µg/mL). Analyses were performed before and after culture to evaluate tissue integrity by classical histology, apoptosis by TUNEL assay, DNA laddering kit and the detection of activated caspase 3, and DNA damage by the immune detection of phosphorylated histone H2A.x (H2AXph139). Both DXR and PTX reduced the number of morphologically normal primordial and developing follicles. Positive staining for TUNEL and active caspase 3 was detected in all the samples (P < 0.05). Therefore, we propose the in situ culture of caprine preantral follicles as a useful experimental model for assessing the toxic effects of the chemotherapeutic agents on ovarian folliculogenesis. Microsc. Res. Tech. 79:773-781, 2016. © 2016 Wiley Periodicals, Inc.

There is about (12772) Related Products to In situ cultured preantral follicles is a useful model to evaluate the effect of anticancer drugs on caprine folliculogenesis.

 EpiQuik In Situ H3K9 Ac  EpiQuik In Situ Histone EpiQuik In Situ Histone H EpiQuik In Situ Histone H EpiQuik In Situ Histone H FitAmp Blood and Cultured  FitAmp Blood and Cultur FitAmp Blood and Cultured  FitAmp Blood and Cultur DirectPCR Lysis Reagent ( DirectPCR Lysis Reagent ( AccuzolTM Total RNA Extra

paperclip

#28565839   2017/06/01 To Up

miR-365 induces hepatocellular carcinoma cell apoptosis through targeting Bcl-2.

Hepatocellular carcinoma (HCC) is currently ranked as the third leading cause of cancer-related mortality worldwide. microRNAs (miRs) serve important roles in the development and progression of HCC. miR-365 has been demonstrated to function as a tumor suppressor in several types of cancer, including HCC; however, the mechanisms by which miR-365 regulates HCC apoptosis remains to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction was performed to determine miR-365 expression levels in HCC and normal liver (LO2) cells. miR-365 overexpression was induced in SMC7721 cells using a plasmid-based system, and Cell Counting Kit-8 and TUNEL assays were performed to detect cell activity and apoptosis following miR-365 transfection. A luciferase assay was performed to determine the direct target of miR-365 in apoptosis regulation. Furthermore, a subcutaneously transplanted tumor model was established to evaluate the effects of miR-365 on tumor growth in vivo. The tumor tissue was used for further proliferation and apoptosis detection. The results of the present study indicated that miR-365 expression was significantly lower in HCC cells compared with LO2 cells (P<0.01). Transfection of SMC7721 cells with miR-365 plasmid significantly inhibited cell activity by inducing apoptosis (P<0.01). Luciferase assay indicated that miR-365 targets B-cell lymphoma 2 (Bcl-2) directly and therefore induces the downstream expression of pro-apoptotic proteins. The SMC7721 primary tumor growth was significantly reduced by miR-365 transfection (P<0.01). Further investigation demonstrated that the miR-365 group contained significantly fewer cells that were positive for proliferating cell nuclear antigen (P<0.01) and significantly more apoptotic cells (P<0.01). In conclusion, the results of the present study demonstrated that miR-365 may serve a role in inducing HCC apoptosis via directly targeting Bcl-2. This may provide a novel diagnosis and therapy target for the treatment of patients with HCC.

There is about (17075) Related Products to miR-365 induces hepatocellular carcinoma cell apoptosis through targeting Bcl-2.

Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Liver hepatocellular carc Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Hepatocellular carcinoma Normal liver and hepatoce Lung large cell carcinoma Hepatocellular carcinoma Multiple non small cell l

paperclip

#28251077   2017/03/02 To Up

The effects of anti-vascular endothelial growth factor agents on human retinal pigment epithelial cells under high glucose conditions.

To investigate the effects of high glucose levels and anti-vascular endothelial growth factor (VEGF) agents (bevacizumab, ranibizumab and aflibercept) on retinal pigment epithelium (RPE) cells.

There is about (6906) Related Products to The effects of anti-vascular endothelial growth factor agents on human retinal pigment epithelial cells under high glucose conditions.

Human Vascular Endothelia Human Vascular Endothelia Recombinant Human Vascula Human Endocrine Gland Vas Rat Vascular Endothelial Mouse Vascular Endothelia Mouse Vascular Endothelia Mouse Vascular Endothelia Human Retinal Microvascul RFP Expressing Human Reti GFP Expressing Human Reti Epidermal Growth Factor (

paperclip

#28531803   2017/05/22 To Up

Isofraxidin inhibited proliferation and induced apoptosis via blockage of Akt pathway in human colorectal cancer cells.

Isofraxidin (IF), a natural coumarin compound, has been reported to possess anti-cancer activity in human liver cancer. However, whether IF is involved in the regulation of colorectal cancer tumorigenesis and development has been not well elucidated.

There is about (29259) Related Products to Isofraxidin inhibited proliferation and induced apoptosis via blockage of Akt pathway in human colorectal cancer cells.

Cancer Apoptosis Phospho- Apoptosis antibody array Cancer samples: Colorect Colorectal cancer tissue SRE Reporter - HEK293 Cel JAK pathway ISRE reporter AP-1 Reporter – HEK293 Nrf antioxidant pathway A Wnt Signaling Pathway TCF Colorectal (colon and rec Colorectal (colon and rec Epidermal Growth Factor (

paperclip

#28580379   2017/06/05 To Up

Synthesis of 2-Oxo-1, 2-Dihydroquinoline Chemotype with Multiple Attachment Points as Novel Screening Compounds for Drug Discovery.

The 2-oxo-1, 2-dihydroquinoline Chemotype is well represented among screening compound collection. However, the chemical space of 2-oxo-1, 2-dihydroquinoline has not been thoroughly investigated. In this work we report the synthesis of a small but novel 2-oxo-1, 2-dihydroquinoline compound array for screening purposes, especially in drug discovery, possessing three convenient point of diversity.

There is about (16466) Related Products to Synthesis of 2-Oxo-1, 2-Dihydroquinoline Chemotype with Multiple Attachment Points as Novel Screening Compounds for Drug Discovery.

Single Peptoid Ligand Syn Peptoid Library Synthesis Peptoid Ligand Discovery Peptoid Ligand Assay Deve Peptoid Ligand Affinity O Caspase 3 Inhibitor Drug PLTP Inhibitor Drug Scree HDAC Inhibitor Drug Scree Caspase 10 Inhibitor Drug Caspase 9 Inhibitor Drug Caspase 1 Inhibitor Drug Caspase 7 Inhibitor Drug

paperclip

#23891081   2013/08/19 To Up

Relationship between intracortical electrode design and chronic recording function.

Intracortical electrodes record neural signals directly from local populations of neurons in the brain, and conduct them to external electronics that control prosthetics. However, the relationship between electrode design, defined by shape, size and tethering; and long-term (chronic) stability of the neuron-electrode interface is poorly understood. Here, we studied the effects of various commercially available intracortical electrode designs that vary in shape (cylindrical, planar), size (15 μm, 50 μm and 75 μm), and tethering [electrode connections to connector with (tethered) and without tethering cable (untethered)] using histological, transcriptomic, and electrophysiological analyses over acute (3 day) and chronic (12 week) timepoints. Quantitative analysis of histological sections indicated that Michigan 50 μm (M50) and Michigan tethered (MT) electrodes induced significantly (p < 0.01) higher glial scarring, and lesser survival of neurons in regions of blood-brain barrier (BBB) breach when compared to microwire (MW) and Michigan 15 μm (M15) electrodes acutely and chronically. Gene expression analysis of the neurotoxic cytokines interleukin (Il)1 (Il1α, Il1β), Il6, Il17 (Il17a, Il17b, Il17f), and tumor necrosis factor alpha (Tnf) indicated that MW electrodes induced significantly (p < 0.05) reduced expression of these transcripts when compared to M15, M50 and FMAA electrodes chronically. Finally, electrophysiological assessment of electrode function indicated that MW electrodes performed significantly (p < 0.05) better than all other electrodes over a period of 12 weeks. These studies reveal that intracortical electrodes with smaller size, cylindrical shape, and without tethering cables produce significantly diminished inflammatory responses when compared to large, planar and tethered electrodes. These studies provide a platform for the rational design and assessment of chronically functional intracortical electrode implants in the future.

There is about (20464) Related Products to Relationship between intracortical electrode design and chronic recording function.

OXYGEN ELECTRODE, 3M CABL Anti 3 DG imidazolone Mon NAT1 Pre-design Chimera R AARS Pre-design Chimera R AADAC Pre-design Chimera SERPINA3 Pre-design Chime NAT2 Pre-design Chimera R NAT1 Pre-design Chimera R A1BG Pre-design Chimera R VEGF-C (Rat, Polyclonal, VEGFR-1 (Human, monoclona VEGF (Mouse, Monoclonal,