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#26726832   2016/01/05 To Up

Fortilin potentiates the peroxidase activity of Peroxiredoxin-1 and protects against alcohol-induced liver damage in mice.

Fortilin, a pro-survival molecule, inhibits p53-induced apoptosis by binding to the sequence-specific DNA-binding domain of the tumor suppressor protein and preventing it from transcriptionally activating Bax. Intriguingly, fortilin protects cells against ROS-induced cell death, independent of p53. The signaling pathway through which fortilin protects cells against ROS-induced cell death, however, is unknown. Here we report that fortilin physically interacts with the antioxidant enzyme peroxiredoxin-1 (PRX1), protects it from proteasome-mediated degradation, and keeps it enzymatically active by blocking its deactivating phosphorylation by Mst1, a serine/threonine kinase. At the whole animal level, the liver-specific overexpression of fortilin reduced PRX1 phosphorylation in the liver, enhanced PRX1 activity, and protected the transgenic animals against alcohol-induced, ROS-mediated, liver damage. These data suggest the presence of a novel oxidative-stress-handling pathway where the anti-p53 molecule fortilin augments the peroxidase PRX1 by protecting it against degradation and inactivation of the enzyme. Fortilin-PRX1 interaction in the liver could be clinically exploited further to prevent acute alcohol-induced liver damage in humans.

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OxiSelect™ Cellular UV- Anti-Alcohol Dehydrogenas Alcohol Dehydrogenase Act Glutathione Peroxidase Ac Alkaline Phospatase (ALP) 4 Hydroxy 3 methoxybenzyl anti-Peroxiredoxin VI (1A anti-Peroxiredoxin-SO3 (1 anti-Peroxiredoxin V (3F1 anti-Peroxiredoxin Ⅲ (4 anti-Peroxiredoxin Ⅲ (2 anti-Peroxiredoxin Ⅰ (1

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#25633985   2015/01/30 To Up

The role of the mammalian DNA end-processing enzyme polynucleotide kinase 3'-phosphatase in spinocerebellar ataxia type 3 pathogenesis.

DNA strand-breaks (SBs) with non-ligatable ends are generated by ionizing radiation, oxidative stress, various chemotherapeutic agents, and also as base excision repair (BER) intermediates. Several neurological diseases have already been identified as being due to a deficiency in DNA end-processing activities. Two common dirty ends, 3'-P and 5'-OH, are processed by mammalian polynucleotide kinase 3'-phosphatase (PNKP), a bifunctional enzyme with 3'-phosphatase and 5'-kinase activities. We have made the unexpected observation that PNKP stably associates with Ataxin-3 (ATXN3), a polyglutamine repeat-containing protein mutated in spinocerebellar ataxia type 3 (SCA3), also known as Machado-Joseph Disease (MJD). This disease is one of the most common dominantly inherited ataxias worldwide; the defect in SCA3 is due to CAG repeat expansion (from the normal 14-41 to 55-82 repeats) in the ATXN3 coding region. However, how the expanded form gains its toxic function is still not clearly understood. Here we report that purified wild-type (WT) ATXN3 stimulates, and by contrast the mutant form specifically inhibits, PNKP's 3' phosphatase activity in vitro. ATXN3-deficient cells also show decreased PNKP activity. Furthermore, transgenic mice conditionally expressing the pathological form of human ATXN3 also showed decreased 3'-phosphatase activity of PNKP, mostly in the deep cerebellar nuclei, one of the most affected regions in MJD patients' brain. Finally, long amplicon quantitative PCR analysis of human MJD patients' brain samples showed a significant accumulation of DNA strand breaks. Our results thus indicate that the accumulation of DNA strand breaks due to functional deficiency of PNKP is etiologically linked to the pathogenesis of SCA3/MJD.

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Anti beta3 AR Human, Poly MarkerGeneTM Chemilumines pDC57 Mammalian Luciferas pDC99 Mammalian Luciferas Mouse Anti-Protein Kinase Alkaline Phospatase (ALP) EnzyChrom™ Kinase Assay Type II 5-phosphatase ant Anti-ACE-1 (Angiotension Anti-ACE-2 (Angiotension- Anti-ACE-1 (Angiotension Anti-ACE-1 (Angiotension

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#24533825   2014/03/04 To Up

Proteomic analysis of human osteoarthritis synovial fluid.

Osteoarthritis is a chronic musculoskeletal disorder characterized mainly by progressive degradation of the hyaline cartilage. Patients with osteoarthritis often postpone seeking medical help, which results in the diagnosis being made at an advanced stage of cartilage destruction. Sustained efforts are needed to identify specific markers that might help in early diagnosis, monitoring disease progression and in improving therapeutic outcomes. We employed a multipronged proteomic approach, which included multiple fractionation strategies followed by high resolution mass spectrometry analysis to explore the proteome of synovial fluid obtained from osteoarthritis patients. In addition to the total proteome, we also enriched glycoproteins from synovial fluid using lectin affinity chromatography.

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Single Donor Synovial Flu Human Synovial Microvascu Synovial sarcoma and canc Bouin's Fluid Bouin's Fluid Bouin's Fluid TOXOPLASMA GONDII Culture Single Donor Cerebrospina Michel's Transport Fluid Human Ascites Fluid 25ml Human Pleural Fluid 100ml Human Ascites Fluid 10ml

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#28494841   2017/05/12 To Up

Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression.

We studied effects of nutrient quercetin on cytochromes' Р450 1А (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

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Anti-Conjugated Benzo(a)p Quercetin, Dihydrate Quercetin, Dihydrate Quercetin CAS Number [117 Quercetin dihydrate CAS N 7-Aminobenzo[a]pyrene C20 7-Aminosuccinylbenzo[a]py Pyrene N Hexadecylsulfona Pyrene 1 sulfonyl chlorid Pyrene 8 hydroxy 1,4,6 tr Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu

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#19194078   2009/02/05 To Up

Quantitative analysis of mRNA transcripts of Hox, SHH, PTCH, Wnt, and Fzd genes in canine hematopoietic progenitor cells and various in vitro colonies differentiated from the cells.

Homeobox (Hox), Sonic hedgehog (SHH), and Wingless-type MMTV integration site family (Wnt) are known to modulate the self-renewal and expansion of hematopoietic progenitor/stem cells in humans and mice. Frizzled (Fzd) and Patched1 (PTCH1) represent the receptors of Wnt and SHH, respectively. In this study, the amounts of mRNA transcripts of the genes associated with the self-renewal of hematopoietic stem cells, HoxB3, HoxB4, HoxA10, Wnt5a, Wnt2b, Fzd1, Fzd6, SHH, and PTCH1, were measured in canine unfractionated bone marrow cells, CD34-enriched cells, and various colony-forming units in culture (CFU-C). Partial cDNA sequences of these 9 canine genes were determined in this study. Quantitative real-time polymerase chain reaction was employed to indicate their relative amounts of mRNA transcripts. Amounts of mRNA transcripts of HoxB3, HoxA10, PTCH1, and Wnt5a genes in canine CD34-enriched cell fraction were significantly larger than those in the CD34-depleted cell fraction. Amounts of mRNA transcripts of HoxB3, HoxA10, PTCH1, Wnt5a, and Wnt2b genes in various CFU-C cells were significantly smaller than those in the seeded CD34-enriched cell fraction. These results suggested important roles of the products of these genes in self-renewal, expansion, and survival of hematopoietic progenitor cells in dogs as shown in humans and rodents.

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anti CD38 Hematopoietic p anti CD34 Hematopoietic p Transfection Reagents and anti CD45 RA B cells, T c Mouse Anti-Human CD34 Tar Recombinant Canine ApoJ C Recombinant Canine ApoJ C Recombinant Canine ApoJ C Human Cord Blood CD34+ Ce 129 Mouse Embryonic Stem Rat Mesenchymal Stem Cell 1,1'-Dioctadecyl-3,3,3',3

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#26660438   2016/01/28 To Up

3H-1,3-Azaphospholo[4,5-b]pyridines--novel heterocyclic P,N-bridging or hybrid ligands: synthesis and first d(8)-transition metal complexes.

The first 3H-1,3-azaphospholo-pyridines 2a-c were synthesized as racemic mixtures in modest to medium yield by the reaction of N-(2-chloropyrid-3-yl)-trimethylacetimidoyl chloride 1 with RPLi2 (R = Ph, n-Bu, i-Bu), generated from RPH2 and BuLi in THF at -70 °C, and studied with respect to their suitability as ligands (L) in transition metal complexes. Reactions of 2a with group 6 metal(0) pentacarbonyls led to P-coordinated LM(CO)5 complexes 3a-5a (Cr, Mo, W) and the reaction of 2c with (norbornadiene)Mo(CO)4 surprisingly to 4c. [Rh(1,5-COD)Cl]2 and 2a,b, in metal/ligand ratio 1 : 1, furnished LRh(1,5-COD)Cl complexes 6a,b with P-coordination, 6b accompanied by a minor contamination by the bis-coordinated L[Rh(COD)Cl]2 complex 7b. Reactions of 2a,b with [(allyl)PdCl]2 proceeded in THF with dismutation of N-coordinated (allyl)PdCl and formed with 2a a labile crude product [(2a){(allyl)PdCl}1.2(PdCl2)0.8]·C4H8O, with the composition close to L[Pd(allyl)Cl]PdCl2 THF (8a·THF), which converted during crystallization to 9a, whereas 2b directly formed the N,N'-PdCl2-bridged bis[LPd(allyl)chloride] complex 9b. Conversion of 2b with equimolar amounts of Pd(CH3CN)2Cl2 in THF, or Na2PdCl4 in methanol, gave rise to the dimeric P,N-bridging complex 10b. Crystal structure analyses of 6a (rac), 9b·2CDCl3 (meso), 10b·4.5THF and 10b·2D6-acetone (rac) provided detailed structural information. 10b, but more efficiently complexes formed in situ from 2a,b and Pd2(DBA)3 or Pd(OAc)2, catalysed the arylamination of 2-bromopyridine with 2,4,6-trimethylaniline.

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#28406288   2017/04/13 To Up

Tin(IV) Compounds with 2-C6F4PPh2 Substituents and Their Reactivity toward Palladium(0): Formation of Tin-Palladium Complexes via Oxidative Addition.

The tin(IV) compounds MexSn(2-C6F4PPh2)4-x (1, x = 1; 2, x = 2) and ClSn(2-C6F4PPh2)3 (3) were obtained from the reactions of 2-LiC6F4PPh2 with MeSnCl3 (3:1), Me2SnCl2 (2:1), or SnCl4 (3:1), respectively. The reactions of 2-LiC6F4PPh2 with SnCl4 in different stoichiometric ratios (4:1-1:1) gave 3 as the main product. Compound Cl2Sn(2-C6F4PPh2)2 (4) was formed in the transmetalation reaction of 3 and [AuCl(tht)] but could not be isolated. 1 and 2 react with palladium(0) sources {[Pd(PPh3)4] and [Pd(allyl)Cp]} by the oxidative addition of one of their Sn-CAryl bonds to palladium(0) with formation of the heterobimetallic complexes [MeSn(μ-2-C6F4PPh2)2Pd(κC-2-C6F4PPh2)] (5) and [Me2Sn(μ-2-C6F4PPh2)Pd(κ(2)-2-C6F4PPh2)] (6) featuring Sn-Pd bonds. The reaction of 3 with palladium(0) proceeds via the oxidative addition of the Sn-Cl bond to palladium(0), thus furnishing the complex [Sn(μ-2-C6F4PPh2)3PdCl] (7) featuring a Sn-Pd bond and a pentacoordinate Pd atom. Transmetalation of MexSn(2-C6F4PPh2)4-x (x = 1-3) with [Pd(allyl)Cl]2 gave MexClSn(2-C6F4PPh2)3-x and [Pd(allyl)(μ-2-C6F4PPh2)]2. For x = 1, the compound MeClSn(2-C6F4PPh2)2 (generated in situ) reacted with another 1 equiv of [Pd(allyl)Cl]2 by the oxidative addition of the Sn-Cl bond to palladium(0) and the reductive elimination of allyl chloride, thus leading to [MeSn(μ-2-C6F4PPh2)2PdCl] (8). The reductive elimination of allyl chloride was also observed in the reaction of 3 with [Pd(allyl)Cl]2, giving [Sn(μ-2-C6F4PPh2)3PdCl] (7). All compounds have been characterized by means of multinuclear NMR spectroscopy, elemental analysis, single-crystal X-ray diffraction, and selected compounds by (119)Sn Mössbauer spectroscopy. Computational analyses (natural localized molecular orbital calculations) have provided insight into the Sn-Pd bonding of 5-8.

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#27996067   2016/12/20 To Up

Dinuclear oxidative addition reactions using an isostructural series of Ni2, Co2, and Fe2 complexes.

A family of low-valent Ni2, Co2, and Fe2 naphthyridine-diimine (NDI) complexes is presented. Ligand-based π* orbitals are sufficiently low-lying to fall within the metal 3d manifold, resulting in electronic structures that are highly delocalized across the conjugated [NDI]M2 system. This feature confers stability to metal-metal interactions during two-electron redox reactions, as demonstrated in a prototypical oxidative addition of allyl chloride.

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#26376799   2015/09/17 To Up

DFT studies on the palladium-catalyzed dearomatization reaction between naphthalene allyl chloride and allyltributylstannane.

The Pd-catalyzed dearomatization of naphthalene allyl chloride with allyltributylstannane has been investigated using density functional theory (DFT) calculations at the B3LYP level. The calculations indicate that the (ŋ(1)-allyl)(ŋ(3)-allyl)Pd(PH3) complex is responsible for the formation of ortho-dearomatized product. Moreover it is easy to produce the ortho-dearomatized product when reductive elimination starts from (ŋ(3)-allylnaphthalene)(ŋ(1)-allyl)Pd complex 7, while it is easy to form the para-dearomatized product when reductive elimination starts from (ŋ(3)-allylnaphthalene)(ŋ(1)-allyl)Pd complex 9. The Stille coupling products can't be produced due to high reaction energy barrier. Graphical Abstract Two mechanisms of dearomatization are investigated by DFT, and (ŋ(1)-allyl)(ŋ(3)-allyl)Pd(PH3) complexes are the main intermediates for ortho-dearomatized product.

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#28347960   2017/03/28 To Up

Pretreatment of eucalyptus with recycled ionic liquids for low-cost biorefinery.

It is urgent to develop recycled ionic liquids (ILs) as green solvents for sustainable biomass pretreatment. The goal of this study is to explore the availability and performance of reusing 1-allyl-3-methylimidazolium chloride ([amim]Cl) and 1-butyl-3-methylimidazolium acetate ([bmim]OAc) for pretreatment, structural evolution, and enzymatic hydrolysis of eucalyptus. Cellulose enzymatic digestibility slightly decreased with the increased number of pretreatment recycles. The hydrolysis efficiencies of eucalyptus pretreated via 4th recycled ILs were 54.3% for [amim]Cl and 72.8% for [bmim]OAc, which were 5.0 and 6.7-folds higher than that of untreated eucalyptus. Deteriorations of ILs were observed by the relatively lower sugar conversion and lignin removal from eucalyptus after 4th reuse. No appreciable changes in fundamental framework and thermal stability of [amim]Cl were observed even after successive pretreatments, whereas the anionic structure of [bmim]OAc was destroyed or replaced. This study suggested that the biomass pretreatment with recycled ILs was a potential alternative for low-cost biorefinery.

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Nycodenz, non ionic, non Octyl â D 1 thioglucopyr