Product name :pOET sequencing primers

Catalog Number :200100

Quantity :2 x 100

Price :176.841 Eur

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Supplier :OET

pOET Sequencing primers


Product Information
Cat. No. : 200100
Storage : -20°C
Shipping : Room Temperature

Description:
The pOET sequencing primers anneal to the upstream and downstream regions of the
multiple cloning site. They can be used to sequence across the MCS or amplify an
insert within it by PCR. These primers are single-stranded oligonucleotides with 5'-
hydroxyl and 3'-hydroxyl ends. All primers are supplied as 10 μM aqueous solutions

pOET-F forward sequencing primer, 32-mer
5'-CAA ACT AAT ATC ACA AAC TGG AAA TGT CTA TC-3'

Tm (50 mM Na+): 52°C
Molecular Weight (μg/:μmol): 9769.4

pOET-R reverse sequencing primer, 29-mer
5'-TAC AAC AAT TGT CTG TAA ATC AAC AAC GC -3'


Tm (50 mM Na+): 51°C
Molecular Weight (μg/:μmol): 8838.8


Concentration
10 μM


Volume
2 x 100 μl

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