Product name :Rat Mesenchymal Replenisher Kit

Catalog Number :5000-001-R

Quantity :500/50 ml

Price :172 Eur

Pay now with :

Supplier :Trevigen

 

Cultrex Rat Mesenchymal Replenisher Kit
Catalog #: 5000-001-R

Rat Mesenchymal Stem Cells

FEATURES:

  • Primary Mesenchymal Stem Cells isolated and purified from Rat Bone Marrow.
  • Qualified medium and fetal bovine serum to support undifferentiated growth of rat mesenchymal stem cells.

APPLICATIONS :

Therapeutic and basic research, including transplantation studies and studies examining the repair of cardiac tissue, bone, cartilage, and tendons often using 3-D matrices

Mesenchymal Stem Cells (MSC), also known as marrow stromal cells, are a self-renewing population of multipotent cells present in bone marrow and many other adult tissues. MSC can be isolated from bone marrow by adherence to plastic, and can differentiate into multiple lineage- specific cells that form bone, fat, cartilage, muscle, neuronal cells and tendon. Due to their multilineage potential, they can be useful tools for a wide range of therapeutic and basic research, including transplantation studies and studies examining the repair of cardiac tissue, bone, cartilage, and tendons often using 3-D matrices. The generation of dependable information that can be shared between laboratories requires a consistent, highly purified source of MSC that have maintained their multipotency, as demonstrated by their ability to differentiate to different tissue types. Isolation of MSC is both a time and resource consuming process which can now be eliminated. A readily available and qualified source of cells will substantially decrease MSC preparation time required prior to experimentation.

Trevigen's RMSC are provided as a frozen ampoule containing 1 x 106 passage 3 cells that are greater than 99% pure. These cells can be maintained in an undifferentiated state when grown in Trevigen's qualified RMSC Medium supplemented with RMSC qualified FBS or induced to differentiate into adipogenic or osteogenic phenotype when growth media are supplemented with reagents contained in Trevigen's differentiation kits. Trevigen's RMSC can undergo 10 doublings without alteration in cell morphology or differentiation potential.

STORAGE:

Cultrex® Qualified RMSC Medium 4°C
Cultrex® Qualified RMSC FBS -20°C

REFERENCES:

1. Phinney DG, Prockop DJ. 2007. Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Models of Tissue Repair-Current Views. Stem Cells 25:2896-2902.
2. Kolf CM, Cho E, Tuan RS. 2007. Biology of Adult Mesenchymal Stem Cells: Regulation of Niche, Self-Renewal and Differentiation. Arthritis Research and Therapy 9:204.
3. Javazon EH, Colter DC, Schwarz EJ, Prockop DJ. 2001. Rat Marrow Stromal Cells are More Sensative to Plating Density and Expand More Rapidly from Single-Cell-Derived Colonies than Human Marrow Stromal Cells. Stem Cells 19:219-225.
4. Li Yi, McIntosh K, Chen J, Zhang C, Gao, Q, Borneman J, Ragniski K, Mitchell J, Shen L, Zhang J, Lu D, Chopp M. 2006. Allogeneic bone marrow stromal cells promote glial-axonal remodeling without immunologic sensitization after stroke in rats. Experimental Neurology 198:313-25.

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