Product name :ISOLATE RNA Mini Kit

Catalog Number :BIO-52042

Quantity :10 rxns

Price :53.3925 Eur

Pay now with :

Supplier :Bioline

 

TOTAL RNA ISOLATION FROM

ANIMAL TISSUE

1. Grind upto20mg sample using mortar and pestle with liquid N,. Transfer to 1.5ml tube. Add 450|jl Lysis Buffer R and homogenize. OR

1.     Place sample in suitable container. Add 450[jI Lysis Buffer R and homogenize using homogenizer. Transfer to 1.5ml tube.

2.     Spin: max. speed for 1 min.

3.     Transfer supernatant to Spin Column R1 placed in Collection Tube.

4.     Spin: 10,000 x g for 2 min. Discard Spin Column R1 and SAVE THE FILTRATE.

5.     Add 1 volume 70% ethanol to filtrate and mix well. Transfer to Spin Column R2 placed in Collection Tube.

6.     Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

7.     Add 500pl Wash Buffer AR.

8.     Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

9.     Add 700[jl Wash Buffer BR.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

11.   Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column R2 in 1.5ml Elution Tube.

12.   Add 30-80pi RNase-free water to Spin Column R2 membrane.

13.   Incubate at RT for 1 min.

14.   Spin: 6000 x g for 1 min. to elute RNA.

The isolated RNA is ready for use in downstream applications

 

TOTAL RNA ISOLATION FROM

PLANT TISSUE

1. Grind up to 100mg sample using mortar and pestle with liquid N2. Transfer to 1.5ml tube. Add 450pl Lysis Buffer APR or BPR and homogenize. OR

1.     Place sample in suitable container. Add 450^1 Lysis Buffer APR or BPR. Homogenize using homogenizer. Transfer to 1.5ml tube.

2.     Spin: max. speed for 1 min.

3.     Transfer supernatant to Spin Column PR1 placed in 2ml Collection Tube.

4.     Spin: 10,000 x g for 2 min. Discard Spin Column PR1 and SAVE THE FILTRATE.

5.     Add 1 volume of 70% ethanol to filtrate and mix well. Transfer to Spin Column PR2 placed in Collection Tube.

6.     Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column PR2 in new Collection Tube.

7.     Add 500|jl Wash Buffer APR.

8.     Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column PR2 in new Collection Tube.

9.     Add 650|il Wash Buffer BPR.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column PR2 in new Collection Tube.

11.   Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column PR2 in 1.5ml Elution Tube.

12.   Add 30-80pl RNase-free water directly to Spin Column PR2 membrane.

13.   Incubate at RT for 1 min.

14.   Spin: 6000 x g for 1 min. to elute RNA.

15.   The isolated RNA is ready for use in downstream applications.

TOTAL RNA ISOLATION FROM

EUKARYOTIC CELLS

1.     Harvest up to 5 x 10G cells.

Cells in suspension: Pellet cells at 300 x g for 5 min. Remove supernatant. Cells in monolayer: Remove cell culture medium.

2.     Add 400pl Lysis Buffer R.

3.     Resuspend pellet by pipetting.

4.     Incubate at RT for 3 min.

5.     Transfer supernatant to Spin Column R1 placed in Collection Tube.

6.     Spin: 10,000 x g for 2 min. Discard Spin Column R1 and SAVE THE FILTRATE.

7.     Add 1 volume 70% ethanol, mix well. Transfer to Spin Column R2 placed in new Collection Tube.

8.     Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

9.     Add 500pl Wash Buffer AR.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

11.   Add 700[il Wash Buffer BR.

12.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

13.   Spin: 10,000 x g for 3 min. Discard Collection Tube, place Spin Column R2 in 1.5ml Elution Tube.

14.   Add 30-80pl RNase-free water to Spin Column R2 membrane.

15.   Incubate at RT for 1 min.

16.   Spin: 6000 x g for 1 min. to elute RNA.

17.   The isolated RNA is ready for use in downstream applications.

 

 

TOTAL RNA ISOLATION FROM

BACTERIAL CELLS

1.     Pellet up to 1 x 10s cells at 5000 x g for 4 min. Discard supernatant.

2.     Resuspend cell pellet in 100^1 TE Buffer.

3.     Add 1pl to 6[jl lysozyme solution. Mix by pipetting.

4.     Add 450|jl Lysis Buffer R, vortex vigorously.

5.     Incubate at RT for 3 min.

6.     Transfer to Spin Column R1 placed in Collection Tube.

7.     Spin: 10,000 x g for 2 min. Discard Spin Column R1 and SAVE THE FILTRATE.

8.     Add 1 volume 70% ethanol to filtrate, mix well.

9.     Transfer 650|jl to Spin Column R2 placed in new Collection Tube.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

11.   Transfer any remaining sample from step 9 to same Spin Column R2.

12.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

13.   Add 500pl Wash Buffer AR.

14.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

15.   Add 700mI Wash Buffer BR.

16.   Repeat step 14.

17.   Spin: 10,000 x g for 3 min. Discard Collection Tube, place Spin Column R2 in 1.5ml Elution Tube.

18.   Add 30-80pi RNase-free water to Spin Column R2 membrane.

19.   Incubate at RT for 1 min.

20.   Spin: 6000 x g for 1 min. to elute RNA.

21.   The isolated RNA is ready for use in downstream applications.

 

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K128-100 Annexin V PE Apoptosis Detection Kit100 assays Biovision 100 assays 315 K128-100 Annexin V PE Apoptosis Detection Kit100 assays Biovision 100 assays 315€
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K256-100 Mitochondria Cytosol Fractionation Kit100 assays Biovision 100 assays 314.685 K256-100 Mitochondria Cytosol Fractionation Kit100 assays Biovision 100 assays 314.685€
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K256-25 Mitochondria Cytosol Fractionation Kit25 assays Biovision 25 assays 164.43 K256-25 Mitochondria Cytosol Fractionation Kit25 assays Biovision 25 assays 164.43€
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K264-100 Glutathione (GSH GSSG Total) Assay Kit100 assays Biovision 100 assays 409.185 K264-100 Glutathione (GSH GSSG Total) Assay Kit100 assays Biovision 100 assays 409.185€ Pub
K265-200 Hydrogen Peroxide Assay Kit200 assays Biovision 200 assays 230.58 K265-200 Hydrogen Peroxide Assay Kit200 assays Biovision 200 assays 230.58€ Pub
K266-100 Nuclear Cytosol Fractionation Kit100 assays Biovision 100 assays 314.685 K266-100 Nuclear Cytosol Fractionation Kit100 assays Biovision 100 assays 314.685€
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K266-25 Nuclear Cytosol Fractionation Kit25 assays Biovision 25 assays 164.43 K266-25 Nuclear Cytosol Fractionation Kit25 assays Biovision 25 assays 164.43€
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K267-50 Cytosol Particulate Separation Kit50 assays Biovision 50 assays 276.885 K267-50 Cytosol Particulate Separation Kit50 assays Biovision 50 assays 276.885€ Pub
K268-50 Membrane Protein Extraction Kit50 assays Biovision 50 assays 314.685 K268-50 Membrane Protein Extraction Kit50 assays Biovision 50 assays 314.685€ Pub
K269-500 Mammalian Cell Extraction Kit500 assays Biovision 500 assays 221.13 K269-500 Mammalian Cell Extraction Kit500 assays Biovision 500 assays 221.13€ Pub
K270-50 FractionPREP Cell Fractionation Kit50 assays Biovision 50 assays 400.68 K270-50 FractionPREP Cell Fractionation Kit50 assays Biovision 50 assays 400.68€ Pub
K274-100 Total Antioxidant Capacity (TAC) Assay Kit100 assays Biovision 100 assays 325.08 K274-100 Total Antioxidant Capacity (TAC) Assay Kit100 assays Biovision 100 assays 325.08€ Pub
K280-50 Mitochondrial DNA Isolation Kit50 assays Biovision 50 assays 390.285 K280-50 Mitochondrial DNA Isolation Kit50 assays Biovision 50 assays 390.285€ Pub
K403-50 ApoBrdU IHC DNA Fragmentation Assay Kit50 assays Biovision 50 assays 579 K403-50 ApoBrdU IHC DNA Fragmentation Assay Kit50 assays Biovision 50 assays 579€
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K410-500 Phosphate Colorimetric Assay Kit500 assays Biovision 500 assays 0 K410-500 Phosphate Colorimetric Assay Kit500 assays Biovision 500 assays 0€ Pub
K661-100 Ascorbic Acid Assay Kit100 assays Biovision 100 assays 325.08 K661-100 Ascorbic Acid Assay Kit100 assays Biovision 100 assays 325.08€ Pub
K761-200 Glutathione Reductase Assay Kit200 assays Biovision 200 assays 325.08 K761-200 Glutathione Reductase Assay Kit200 assays Biovision 200 assays 325.08€ Pub
K773-100 Catalase Assay Kit100 assays Biovision 100 assays 230.58 K773-100 Catalase Assay Kit100 assays Biovision 100 assays 230.58€ Pub
K808-200 Deproteinizing Sample Preparation Kit200 assays Biovision 200 assays 231 K808-200 Deproteinizing Sample Preparation Kit200 assays Biovision 200 assays 231€ Pub
LV014 Lenti RNAi Vector Linearized ABM Goods 25ul 271.95 LV014 Lenti RNAi Vector Linearized ABM Goods 25ul 271.95€ Pub
LV015 Lenti RNAi Scramble Vector ABM Goods 500ng 130.2 LV015 Lenti RNAi Scramble Vector ABM Goods 500ng 130.2€
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LV015-G Lenti RNAi Scramble Vector with GFP ABM Goods 500ng 130.2 LV015-G Lenti RNAi Scramble Vector with GFP ABM Goods 500ng 130.2€
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LV016 Lenti EGFP RNAi Vector Linearized ABM Goods 25ul 271.95 LV016 Lenti EGFP RNAi Vector Linearized ABM Goods 25ul 271.95€ Pub
MD-14-0252P Recombinant Human SCF KITLG Proteins Ray Biotech 10 309 MD-14-0252P Recombinant Human SCF KITLG Proteins Ray Biotech 10 309€
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MD-14-0253P Recombinant Mouse SCF KITLG Proteins Ray Biotech 10 309 MD-14-0253P Recombinant Mouse SCF KITLG Proteins Ray Biotech 10 309€
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MD-14-0255P Recombinant Human SDF-1a KITLG Proteins Ray Biotech 10 309 MD-14-0255P Recombinant Human SDF-1a KITLG Proteins Ray Biotech 10 309€
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MGLTB100 Mini Glass Tubes for handheld readers (100 bag) Bioassays 100 tests x 2 60 MGLTB100 Mini Glass Tubes for handheld readers (100 bag) Bioassays 100 tests x 2 60€ Pub
MS7-UV10 Multi Sub Mini, 7 x 10cm UV Tray Cleaver 76 MS7-UV10 Multi Sub Mini, 7 x 10cm UV Tray Cleaver 76€ Pub
MS7-UV7 Multi Sub Mini, 7 x 7cm UV Tray Cleaver 71 MS7-UV7 Multi Sub Mini, 7 x 7cm UV Tray Cleaver 71€ Pub
MSMINI10 Multi Sub Mini, 7 x 10cm UV Tray, 2 x 8 sample, 1mm thick combs, casting dams, loading guides Cleaver 1 271 MSMINI10 Multi Sub Mini, 7 x 10cm UV Tray, 2 x 8 sample, 1mm thick combs, casting dams, loading guides Cleaver 1 271€ Pub
MSMINI7 Multi Sub Mini, 7 x 7cm UV Tray, 2 x 8 sample, 1mm thick combs, casting dams, loading guides Cleaver 1 271 MSMINI7 Multi Sub Mini, 7 x 7cm UV Tray, 2 x 8 sample, 1mm thick combs, casting dams, loading guides Cleaver 1 271€ Pub
MSMINIDUO Multi Sub Mini, 7 x 7cm & 7 x 10cm UV Tray & 2 x 8 sample, 1mm thick combs, casting dams, loading guides Cleaver 242 MSMINIDUO Multi Sub Mini, 7 x 7cm & 7 x 10cm UV Tray & 2 x 8 sample, 1mm thick combs, casting dams, loading guides Cleaver 242€ Pub
PBKXL-10 ProPrep™ Genomic XL-10 High-Throughput Genomic DNA Isolation Kits Biotech support group 10, 10ml whole blood 655 PBKXL-10 ProPrep™ Genomic XL-10 High-Throughput Genomic DNA Isolation Kits Biotech support group 10, 10ml whole blood 655€ Pub
PLF-960 ProPrep™ BAC 960 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group 10x96, 2.0ml cultures 1501 PLF-960 ProPrep™ BAC 960 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group 10x96, 2.0ml cultures 1501€ Pub
PPO-200 ProPrep™ Omni 200 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group Up to 200 ml cultures 460 PPO-200 ProPrep™ Omni 200 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group Up to 200 ml cultures 460€ Pub
QAH-CUST Custom Quantibody Ray Biotech 2 Slide minimum 0 QAH-CUST Custom Quantibody Ray Biotech 2 Slide minimum 0€
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QAM-CUST Custom Quantibody Ray Biotech 2 Slide minimum 0 QAM-CUST Custom Quantibody Ray Biotech 2 Slide minimum 0€
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QAR-CUST Custom Quantibody Ray Biotech 2 Slide minimum 0 QAR-CUST Custom Quantibody Ray Biotech 2 Slide minimum 0€
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SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.368 SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.368€ Pub
SLB-01 UltraSlim LED Illuminator(Black) Blue LED illuminator for mini gels Maestro 529 SLB-01 UltraSlim LED Illuminator(Black) Blue LED illuminator for mini gels Maestro 529€
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SLB-01W UltraSlim LED Illuminator(White) Blue LED illuminator for mini gels Maestro 529 SLB-01W UltraSlim LED Illuminator(White) Blue LED illuminator for mini gels Maestro 529€
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Y213986 ABCE1 RNAse L inhibitor ABM Goods 200ul 520.8 Y213986 ABCE1 RNAse L inhibitor ABM Goods 200ul 520.8€
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Y213986 ABCE1 RNAse L inhibitor Antibody ABM Goods 200ul 462 Y213986 ABCE1 RNAse L inhibitor Antibody ABM Goods 200ul 462€
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Y213994 ABCE1 RNase L ABM Goods 200ul 366.45 Y213994 ABCE1 RNase L ABM Goods 200ul 366.45€
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Y213994 ABCE1 RNase L Antibody ABM Goods 200ul 325.5 Y213994 ABCE1 RNase L Antibody ABM Goods 200ul 325.5€
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