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TCR modifications that enhance chain pairing in gene-modified T cells can augment cross-reactivity and alleviate CD8 dependence.

T cell receptor (TCR) gene-modified T cells are a promising immunotherapy but require refinement to improve clinical responses and limit off-target toxicities. A variety of TCR and gene-delivery vector modifications have been developed to enhance introduced TCR expression and limit introduced/endogenous TCR chain mispairing, improving target antigen recognition and minimizing mispairing-induced cross-reactivity. Using our well-characterized HCV1406 TCR, we previously compared the impact of various chain pairing enhancing modifications on TCR expression and cognate antigen recognition. HCV1406 TCR is also natively cross-reactive against naturally occurring altered peptide ligands (APLs), which was shown to be dependent on high TCR surface density. In this report, we observed in a Jurkat model that absent TCR chain pairing competition alleviated CD8-dependent APL recognition and induced novel cross-reactivity of HCV1406 TCR. We then compared chain pairing enhancing modifications' effects on TCR cross-reactivity in Jurkat and T cells, showing C-terminal leucine zippers and constant region murinization alleviated CD8 dependence and induced novel APL recognition. While modifications enhancing TCR chain pairing intend to avoid cross-reactivity by limiting mispairing with the endogenous TCR, these data suggest they may also enhance natural cross-reactivity and reduce dependence on CD8. These observations have significant implications on the design/implementation of TCR gene-modified T cells.

1576 related Products with: TCR modifications that enhance chain pairing in gene-modified T cells can augment cross-reactivity and alleviate CD8 dependence.

Interferon-a Receptor Typ Rat TGF-beta-inducible ea Rat TGF-beta-inducible ea Glucagon ELISA KIT, Rat G Leptin ELISA Kit, Rat Lep Top five cancer tissue ar Multiple organ cancer tis Lung cancer tissue array, Lung cancer tissue array Colon cancer and normal t Colon cancer and normal t Colon cancer, metastasize

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Epidemiological Investigation and Genotype of Chlamydia Exposure in Pigeons in Three Provinces in Northern China.

Chlamydia is considered as one of the most widely prevalent zoonotic pathogens. It can spread from infected birds to human beings through direct or indirect contact with fecal shedding of Chlamydia. However, data concerning prevalence and genotypes of Chlamydia in pigeons are limited. In the present study, a total of 963 serum samples was collected from Jilin Province, Liaoning Province, and Inner Mongolia Autonomous Region (IMAR) in China between August 2015 and December 2016 and the seroprevalence for Chlamydia was analyzed by indirect hemagglutination assay test. The seroprevalence of Chlamydia was 20.4% (215/963) in total, at the cutoff 1:16, with the titers of 1:16 in 109, 1:64 in 49, 1:256 in 38, and 1:1024 in 18. Samples from all six administrative cities were detected Chlamydia-seropositive, ranging from 19.0% to 25.0%. Adult pigeons (23.5%) have a significant higher seroprevalence than juveniles (15.2%). Four PCR-positive samples represented Chlamydia psittaci genotype B. This is the first report of Chlamydia infection in pigeons in Liaoning Province and IMAR. The occurrence of C. psittaci genotype B in the droppings of pigeons suggests potential environmental contamination with C. psittaci and may raise a public health concern.

2001 related Products with: Epidemiological Investigation and Genotype of Chlamydia Exposure in Pigeons in Three Provinces in Northern China.

Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser ING1B antisense ING1B sense Interferon γ p19 INK4D AKT1 (dn) Inducible Insulin

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Rickettsia africae and Novel Rickettsial Strain in Amblyomma spp. Ticks, Nicaragua, 2013.

We report molecular detection of Rickettsia africae in Amblyomma ovale ticks from Nicaragua and a novel rickettsial strain in an A. triste tick. Of 146 ticks from dogs, 16.4% were Rickettsia PCR positive. The presence of Rickettsia spp. in human-biting ticks in Nicaragua may pose a public health concern.

1672 related Products with: Rickettsia africae and Novel Rickettsial Strain in Amblyomma spp. Ticks, Nicaragua, 2013.

Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Cell Strainers 40μm Cell Cell Strainers 70μm Cell Cell Strainers 100μm Cel Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser ING1B antisense ING1B sense Interferon γ

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Human African Trypanosomiasis in Emigrant Returning to China from Gabon, 2017.

Human African trypanosomiasis is endemic to parts of sub-Saharan Africa and should be considered in the differential diagnosis of patients who have visited or lived in Africa. We report a 2017 case of stage 2 Trypanosoma brucei gambiense disease in an emigrant who returned to China from Gabon.

1885 related Products with: Human African Trypanosomiasis in Emigrant Returning to China from Gabon, 2017.

Recombinant Human Interfe Goat Anti-Human TOM1L1 SR Rabbit Anti-Human Toll In Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon CELLKINES Natural Human I Human Interleukin-4 IL-4 Human Interleukin-6 IL-6 Human Interleukin-7 IL-7 Human Interleukin-2 IL-2 Human Macrophage Inflamma Human Macrophage Inflamma

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Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.

Although the detection of Toxoplasma gondii in bovine tissues is rare, beef might be an important source of human infection. The use of molecular techniques, such as magnetic capture qPCR (MC-qPCR), in combination with the gold standard method for isolating the parasite (mouse bioassay), may increase the sensitivity of T. gondii detection in infected cattle. The risk of transmission of the parasite to humans from undercooked/raw beef is not fully known and further knowledge about the predilection sites of T. gondii within cattle is needed. In the current study, six Holstein Friesian calves (Bos taurus) were experimentally infected with 106 T. gondii oocysts of the M4 strain and, following euthanasia (42 dpi), pooled tissues were tested for presence of the parasite by mouse bioassay and MC-qPCR.

1281 related Products with: Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story.

Multiple organ tumor tiss Toxoplasma gondii GRA8, r Toxoplasma gondii MIC 3 r Toxoplasma gondii P24 (GR Toxoplasma gondii P29 (GR Toxoplasma gondii P30 (SA anti HSV (II) gB IgG1 (mo anti HCMV IE pp65 IgG1 (m anti HCMV gB IgG1 (monocl Anti-HBeAg (HBeAb) test s Anti-HBcAg (HBcAb) test s HCV antibody test strip,

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Predictability of escape for a stochastic saddle-node bifurcation: When rare events are typical.

Transitions between multiple stable states of nonlinear systems are ubiquitous in physics, chemistry, and beyond. Two types of behaviors are usually seen as mutually exclusive: unpredictable noise-induced transitions and predictable bifurcations of the underlying vector field. Here, we report a different situation, corresponding to a fluctuating system approaching a bifurcation, where both effects collaborate. We show that the problem can be reduced to a single control parameter governing the competition between deterministic and stochastic effects. Two asymptotic regimes are identified: When the control parameter is small (e.g., small noise), deviations from the deterministic case are well described by the Freidlin-Wentzell theory. In particular, escapes over the potential barrier are very rare events. When the parameter is large (e.g., large noise), such events become typical. Unlike pure noise-induced transitions, the distribution of the escape time is peaked around a value which is asymptotically predicted by an adiabatic approximation. We show that the two regimes are characterized by qualitatively different reacting trajectories with algebraic and exponential divergences, respectively.

1934 related Products with: Predictability of escape for a stochastic saddle-node bifurcation: When rare events are typical.

MOUSE ANTI BOVINE ROTAVIR Bone Morphogenetic Protei Growth Differentiation Fa Amplite™ Fluorimetric F Goat Anti-Human, Mouse AR MOUSE ANTI BORRELIA BURGD succinate-CoA ligase, GDP formin-like 1 antibody So succinate-CoA ligase, ADP Primary antibody Caspase Primary antibody FLIP An TC Treated Flasks, 250ml,

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Viral and Antibody Kinetics, and Mosquito Infectivity of an Imported Case of Zika Fever Due to Asian Genotype (American Strain) in Singapore.

We report a case of a Singaporean who acquired Zika virus (ZIKV) during a visit to Cuba. The infection was confirmed using molecular and serological methods. This report highlights potential drawbacks of using IgG serology for diagnosis of flavivirus infections in endemic regions. The low viremia detected during the early phase of this case resulted in low mosquito infectivity rates, suggesting the possibility of ZIKV transmission prior to clinical onset. The report also emphasizes the challenges of public health interventions for Zika fever and the importance of sustaining a low vector population to reduce the risk of arbovirus transmission in vulnerable regions.

1881 related Products with: Viral and Antibody Kinetics, and Mosquito Infectivity of an Imported Case of Zika Fever Due to Asian Genotype (American Strain) in Singapore.

MOUSE ANTI BOVINE ROTAVIR Androgen Receptor (Phosph Androgen Receptor (Phosph Androgen Receptor (Ab 650 Viral antibodies, anti-H Bladder cancer tissue arr Breast cancer tissue arra Breast cancer tissue arra Colon carcinoma antibody Colon carcinoma tissue ar Cervix carcinoma for anti Cervix cancer tissue arra

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Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length.

By using human genomic DNA as a template to clone protection of telomere 1 (POT1) promoter gene segments and construct the POT1 promoter luciferase report gene vector (pGL3-Control-POT1-promoter), the association between POT1, and the regulation of telomerase and telomere length was investigated. In the present study, two recombinant luciferase report gene vectors were constructed, which included different regions of the POT1 promoter. The plasmids were transformed into DH5α and the positive clones were obtained. The two plasmids termed as pGL3-Control-POT1-promoter-1 and pGL3-Control-POT1-promoter-2, were confirmed using restriction enzyme analysis and sequencing. They were separately and transiently transfected into four types of human tumor cells (A549, H460, HepG2 and HeLa). The transcriptional activities of the POT1 promoter were verified using the dual-luciferase assay. The relative expression of POT1 and human telomerase reverse transcriptase (hTERT), and telomere length were analyzed using quantitative polymerase chain reaction in the four types of non-transfected tumor cells. Using SPSS software, correlations between POT1 promoter activity, and POT1 expression, hTERT expression and telomere length were analyzed. Two POT1 promoter fragments (POT1-promoter-1 and -2) were successfully constructed into the pGL3-Control luciferase report gene vector. POT1-promoter-1 exhibited significantly stronger transcription activity compared with POT1-promoter-2. The results of the partial correlation and linear regression analyses were similar: POT1 promoter activity was identified to be significantly and positively correlated with POT1 expression and telomere length (partial correlation coefficients, both P<0.05; linear regression, both P<0.01). However, POT1 promoter activity and hTERT expression were significantly negatively correlated (both P<0.05). The results obtained in the present study suggest that the POT1 promoter influences telomere length. Furthermore, these data indicated that POT1 promoter activity and POT1, as well as telomere length, may be a useful biomarker for tumor detection and future patient prognosis.

2731 related Products with: Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length.

AZD-3514 Mechanisms: Andr Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss MultiGene Gradient therm Insulin promoter factor 1 BACTERIOLOGY BACTEROIDES

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Antitumor effect of a new nano-vector with miRNA-135a on malignant glioma.

MiR-135a is found to selectively induce apoptosis in glioma cell but not in normal neurons and glial cells. However, low transfection efficacy limits its application in vivo as other miRNAs. We prepared a new kind of nano-vector based on polyethylene glycol methyl ether (mPEG) and hyper-branched polyethylenimine (hy-PEI) in order to improve the miRNA delivery system into the glioma cells.

1671 related Products with: Antitumor effect of a new nano-vector with miRNA-135a on malignant glioma.

G418 Sulfate (siRNA Vecto G418 Sulfate (siRNA Vecto RAP2C, member of RAS onco pGB AIF siRNA Vector Mix pGB AIF siRNA Vector Mix Human miRNA array I Cancer miRNA array Stem cell miRNA Array Benz[j]aceanthrylen-2(1H) Benz[j]aceanthrylen-2(1H) Skin malignant tumor tiss Skin malignant tumor tiss

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PEG-Poly(amino acid)s/MicroRNA Complex Nanoparticles Effectively Arrest the Growth and Metastasis of Colorectal Cancer.

One of the biggest challenges in developing microRNA (miRNA) based therapeutics is the method of delivery. Herein we report the design and synthesis of mPEG-poly(amino acid)s, which we used as a novel nanocarrier for the delivery of miRNA-139-5p. The PEG-poly(amino acid)s/miRNA-139-5p nanoparticle complex is more effective at suppressing tumor growth and migration in mice with colorectal cancer than when treated with miRNA-139-5p solution and blank nanoparticles individually. Our results suggest that PEG-poly(amino acid)s are a promising non-viral gene vector for the delivery of miRNAs to treat cancers.

1278 related Products with: PEG-Poly(amino acid)s/MicroRNA Complex Nanoparticles Effectively Arrest the Growth and Metastasis of Colorectal Cancer.

Colorectal (colon and rec Colorectal (colon and rec Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Fibroblast Growth Factor Fibroblast Growth Factor DNP X acid [6 (2,4 Dinitr DNP X acid, SE [6 (2,4 Di Primary antibody GFR alp EDANS acid [5 ((2 Aminoet

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