Search results for: miRNASelect™ pEP_hsa_mir_598 Expression Vector
#29055227 2017/10/21 Save this To Up
Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts.Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34(+)/CD41(+) megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research.
2205 related Products with: Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts.Rabbit Anti-Human Androge Rabbit Anti-Human Androge Rabbit Anti-Human Androge Goat Anti-Human Androgen CAR,CAR,Constitutive acti Recombinant Human Androge Human Platelet Derived Gr Human Platelet Derived Gr Human Stromal Cell-Derive Androgen Receptor (Phosph Androgen Receptor (Phosph anti A1, A2 human blood a
#29055225 2017/10/21 Save this To Up
Generation and characterization of a human iPSC line SANi005-A containing the gray platelet associated heterozygous mutation p.Q287* in GFI1B.Peripheral blood mononuclear cells were isolated from an individual harboring a heterozygous c.859C→T p.Q287* mutation in GFI1B, causing an autosomal dominant bleeding disorder, platelet type, 17 (BDPLT17). PBMCs were differentiated to erythroblasts and reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. Pluripotency of iPSC line was confirmed by expression of associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. Normal karyotype confirmed the genomic integrity of iPSCs and the presence of disease causing mutation was shown by Sanger sequencing. The generated iPSCs can be used to study BDPLT17 pathophysiology and basic functions of GFI1B.
1537 related Products with: Generation and characterization of a human iPSC line SANi005-A containing the gray platelet associated heterozygous mutation p.Q287* in GFI1B.Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon anti SLAM anti CDw150 IgG Human Macrophage Inflamma Human Macrophage Inflamma Human Interleukin-32 alph Human Platelet Derived Gr Human Platelet Derived Gr Human Interleukin-1-alpha Interferon-a Receptor Typ Goat Anti-Human Synaptota Goat Anti-Human STK39 SPA
#29053694 2017/10/20 Save this To Up
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae.In vivo microinjection is the most commonly used gene transfer technique for analyzing the gene functions in individual mosquitoes. However, this method requires a more technically demanding operation and involves complicated procedures, especially when used in larvae due to their small size, relatively thin and fragile cuticle, and high mortality, which limit its application. In contrast, viral vectors for gene delivery have been developed to surmount extracellular and intracellular barriers. These systems have the advantages of easy manipulation, high gene transduction efficiency, long-term maintenance of gene expression, and the ability to produce persistent effects in vivo. Mosquito densoviruses (MDVs) are mosquito-specific, small single-stranded DNA viruses that can effectively deliver foreign nucleic acids into mosquito cells; however, the replacement or insertion of foreign genes to create recombinant viruses typically causes a loss of packaging and/or replication abilities, which is a barrier to the development of these viruses as delivery vectors. Herein, we report using an artificial intronic small-RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus (AaeDV) in vivo delivery system. Detailed procedures for the construction, packaging and quantitative analysis of the rAaeDV vectors, and for larval infection are described. This study demonstrates, for the first time, the feasibility of developing a non-defective recombinant MDV micro RNA (miRNA) expression system, and thus providing a powerful tool for the functional analysis of genes in mosquito and establishing a basis for the application of viral paratransgenesis for controlling mosquito-borne diseases. We demonstrated that Aedes albopictus 1(st) instar larvae could be easily and effectively infected by introducing the virus into the water body of the larvae breeding site and that the developed rAaeDVs could be used to overexpress or knock down the expression of a specific target gene in larvae, providing a tool for the functional analysis of mosquito genes.
2567 related Products with: Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae.pCAMBIA2300 Vector (No Re Bone Morphogenetic Protei Growth Differentiation Fa DNA (cytosine 5) methyltr EMAP-II Inhibitor Z-ASTD- EMAP-II Inhibitor Z-ASTD- EMAP II Inhibitor Z ASTD EMAP II Inhibitor Z ASTD Amplite™ Fluorimetric H Amplite™ Intracellular Amplite™ Fluorimetric P Amplite™ Fluorimetric A
#29052760 2017/10/20 Save this To Up
Impact of plasmid architecture on stability and yEGFP3 reporter gene expression in a set of isomeric multicopy vectors in yeast.Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. Considering that rich medium favors growth rate and, simultaneously, is less expensive than selective medium, enhancing stability in non-selective medium is extremely desirable. In this study, we changed the architecture of a multicopy model expression plasmid, creating six isoforms (same size, same DNA content but different positions and orientations of the expression block) and studied mitotic stability, copy number, as well as reporter yEGFP3 expression between isoforms. With one isoform being significantly more stable than the others and another one exhibiting elevated plasmid copy numbers in rich medium, we show that consideration of the arrangement of the plasmid elements might be crucial for productivity employing Saccharomyces cerevisiae as a host. We strongly believe that the ideal architecture has to be assessed for each case and assembly strategy has to begin by evaluating the stability of the vector backbone before insertion of the desired gene. For the plasmid set studied, yEGFP3 reporter production depends more on mitotic stability than on elevated plasmid copy numbers in a small number of cells retaining the plasmid under non-selective conditions.
1630 related Products with: Impact of plasmid architecture on stability and yEGFP3 reporter gene expression in a set of isomeric multicopy vectors in yeast.DNA (cytosine 5) methyltr pCAMBIA0105.1R Vector, (G Instrument Hardware Acces Rabbit Anti-FGF3 Oncogene PTK2 & FLT1 Protein Prote CRKL & SOS1 Protein Prote CRKL & EGFR Protein Prote CCNB1 & PKMYT1 Protein Pr AKT1 & MAPK14 Protein Pro PDGFRB & SLC9A3R1 Protein HSPB1 & F13A1 Protein Pro MAPK3 & MAPK14 Protein Pr
#29052517 2017/10/20 Save this To Up
Constitutive expression of OsDof4, encoding a C2-C2 zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.).Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors which contain a conserved C2-C2-type zinc finger domain, are involved in many fundamental processes. In the Arabidopsis photoperiod response pathway, CDF (CYCLING DOF FACTOR) proteins have a primary role as acting via transcriptional repression of the direct FLOWERING LOCUS T (FT) activator CONSTANS (CO). Our previous study indicated that one of CDF homologs, OsDOf12, was involved in photoperiodic flowering. However, the functional characterization of other rice CDF like genes is still in progress. Here, we characterized the function of OsDof4 in rice.
1581 related Products with: Constitutive expression of OsDof4, encoding a C2-C2 zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.).AZD-8055 Mechanisms: mTOR AZD-2014 Mechanisms: mTOR Human Inflammation Array Human Inflammation Array DNA (cytosine 5) methyltr DABCYL C2 amine Zinc finger CCHC domain c V-ATPase C2 antibody Sour TGF beta induced factor 2 zinc finger protein 574 a hnRNP C1 C2 antibody Sour Transcription factor E3 a
#29051711 2017/10/20 Save this To Up
Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma.Cervical cancer is a major cause of death in women worldwide. Interferon-induced transmembrane protein 1 (IFITM1) is involved in antivirus defense, cell adhesion, and carcinogenesis in different tissues. However, the role of IFITM1 gene in cervical squamous cell cancer is unclear.
2123 related Products with: Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma.Epidermal Growth Factor ( Epidermal Growth Factor ( Lung squamous cell carcin Cervix squamous cell carc Esophagus squamous cell c Esophagus squamous cell c Esophageal squamous cell Esophagus squamous cell c Esophageal squamous cell Oral cavity squamous cell Skin squamous cell carcin GLP 2 ELISA Kit, Rat Prog
#29050481 2017/10/20 Save this To Up
MicroRNA-497 suppress osteosarcoma by targeting MAPK/Erk pathway.The aim of this study was to study the mechanism of miRNA-497 in the apoptosis of osteosarcoma cells.
GPCR Signaling to MAPK ER Erk1 2 MAPK Antibody SRE Reporter - HEK293 Cel ERK Signaling Phospho-Spe MAPK Phospho-Specific Arr Human Mouse Rat Phospho-E ERK2 ERK2 (dn) ERK5 ERK5 (dn) ERK1 ERK2 (flag)
#29050360 2017/10/20 Save this To Up
Immunomodulatory and antitumor effects of type I interferons and their application in cancer therapy.During the last decades, the pleiotropic antitumor functions exerted by type I interferons (IFNs) have become universally acknowledged, especially their role in mediating interactions between the tumor and the immune system. Indeed, type I IFNs are now appreciated as a critical component of dendritic cell (DC) driven T cell responses to cancer. Here we focus on IFN-α and IFN-β, and their antitumor effects, impact on immune responses and their use as therapeutic agents. IFN-α/β share many properties, including activation of the JAK-STAT signaling pathway and induction of a variety of cellular phenotypes. For example, type I IFNs drive not only the high maturation status of DCs, but also have a direct impact in cytotoxic T lymphocytes, NK cell activation, induction of tumor cell death and inhibition of angiogenesis. A variety of stimuli, including some standard cancer treatments, promote the expression of endogenous IFN-α/β, which then participates as a fundamental component of immunogenic cell death. Systemic treatment with recombinant protein has been used for the treatment of melanoma. The induction of endogenous IFN-α/β has been tested, including stimulation through pattern recognition receptors. Gene therapies involving IFN-α/β have also been described. Thus, harnessing type I IFNs as an effective tool for cancer therapy continues to be studied.
1292 related Products with: Immunomodulatory and antitumor effects of type I interferons and their application in cancer therapy.High density (188 cases 2 High density (188 cases 2 Multiple organ cancer and Multiple types of kidney High density (208 cores), Multiple cancer (12 type) Multiple cancer (12 type) Multiple types of cancer Top 4 types of cancer (co Top 4 types of cancer (co MOUSE ANTI BOVINE ROTAVIR Interferon-a Receptor Typ
#29049808 2017/10/19 Save this To Up
Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats.Cold-inducible ribonucleic acid-binding protein (CIRP) has been identified to play a role in the antiapoptotic effect of hypothermia. We sought to investigate the renoprotection of CIRP in a rat model of deep hypothermic circulatory arrest.
1373 related Products with: Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats.Rat intestinal fatty acid Goat Anti-Human Vitamin D Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Mouse Anti-Fatty Acid Bin Prolactin-Inducible Prote AKT1 (dn) Inducible Acyl CoA binding Protein HIV 1 intergase antigen.
#29046446 2017/10/19 Save this To Up
Foamy Virus Vector Carries a Strong Insulator in Its Long Terminal Repeat Which Reduces Its Genotoxic Potential.Strong viral enhancers in γ-retrovirus vectors have caused cellular proto-oncogene activation and leukemia, necessitating use of cellular promoters in 'enhancer-less' self-inactivating integrating vectors. However, cellular promoters result in relatively low transgene expression, often leading to inadequate disease correction. Vectors derived from foamy virus, a nonpathogenic retrovirus, show higher preference for non-genic integrations than γ-retroviruses/lentiviruses and preferential integration near transcriptional start sites, like γ-retroviruses. We found that strong viral enhancer/promoters placed in foamy viral vectors caused extremely low immortalization of primary mouse hematopoietic stem/progenitor cells compared to analogous γ-retrovirus/lentivirus vectors carrying the same enhancer/promoters; an effect not explained solely by foamy virus' modest insertional site preference for non-genic regions, compared to γ-retrovirus/lentivirus vectors. Using CRISPR/Cas9-mediated targeted insertion of analogous proviral sequences into the LMO2 gene and then measuring LMO2 expression, we demonstrated a sequence specific effect of foamy virus, independent of insertional bias, contributing to reduced genotoxicity. We show that this effect is mediated by a 36-bp insulator located in the foamy virus LTR that has high affinity binding for the CCCTC-binding factor. Using our LMO2 activation assay, LMO2 expression was significantly increased when this insulator was removed from foamy virus, and significantly reduced when this insulator was inserted into the lentiviral LTR. Our results elucidate a mechanism underlying the low genotoxicity of foamy virus, identify a novel insulator, and support the use of foamy virus as a vector for gene therapy, especially when strong enhancer/promoters are required.IMPORTANCE Understanding the genotoxic potential of viral vectors is important in designing safe and efficacious vectors for gene therapy. Self-inactivating vectors, devoid of viral long-terminal-repeat enhancers, have proven safe; however, transgene expression from cellular promoters is often insufficient for full phenotypic correction. Foamy virus is an attractive vector for gene therapy. We found foamy virus vectors to be remarkably less genotoxic; well below what is expected from their integration site preferences. We demonstrate that the foamy virus long-terminal-repeats contain an insulator element that binds CCCTC-binding factor and reduce its insertional genotoxicity. Our study elucidates a mechanism behind the low genotoxic potential of foamy virus, identifies a unique insulator, and supports use of foamy virus as a vector for gene therapy.
2054 related Products with: Foamy Virus Vector Carries a Strong Insulator in Its Long Terminal Repeat Which Reduces Its Genotoxic Potential.HA (Influenza A Virus Hem Anti-Infectious Pancreati Anti-Infectious Pancreati Anti-Infectious Pancreati Anti-Infectious Pancreati Avian Influenza virus (H7 Rat anti human Indian Hed Recombinant Hemagglutinin Mouse AntiInfluenza B Nuc pCAMBIA0105.1R Vector, (G Goat Anti-Influenza A Vir Goat Anti-Influenza A Vir
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