Search results for: miRNASelect™ pEP_hsa_mir_125b_2 Expression Vector
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Exploring the role of cellular homologous of the 30K-superfamily of plant virus movement proteins.Genes orthologous to the 30K-superfamily of movement proteins (MP) from plant viruses have been recently discovered by bioinformatics analyses as integrated elements in the genome of most vascular plants. However, their functional relevance for plants is still unclear. Here, we undertake some preliminary steps into the functional characterization of one of these putative MP genes found in Arabidopsis thaliana. We found that the AtMP gene is expressed at different stages of the plant development, with accumulation being highest in flowers but lowest in mature siliques. We also found down-regulation of the gene may result in a small delay in plant development and in an exacerbation of the negative effect of salinity in germination efficiency. We have also explored whether changes in expression of the endogenous AtMP have any effect on susceptibility to infection with several viruses, and found that the infectivity of tobacco rattle tobravirus was strongly dependent on the expression of the endogenous AtMP. Finally, we have cloned the endogenous MP from four different plant species into an expression vector that allows for specifically assessing their activity as cell-to-cell movement proteins and have shown that though some may still retain the ancestral activity, they do so in a quite inefficient manner, thus suggesting they have acquired a novel function during adaptation to the host genome.
2332 related Products with: Exploring the role of cellular homologous of the 30K-superfamily of plant virus movement proteins.Recombinant Human PKC the Recombinant Human PKC the Recombinant Human PKC the Ofloxacin CAS Number [824 BACTERIOLOGY BACTEROIDES TCP-1 theta antibody Sour Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus Recombinant Dengue Virus
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MiR-1224-5p enhances hepatic lipogenesis by targeting Adenosine monophosphate activated protein kinase α1 in male mice.MicroRNAs are potential therapeutic targets for metabolic diseases. Here, miR-1224-5p was highly expressed in the livers of both high fat diet (HFD)-fed mice and obese (ob/ob) mice. To examine the potential role of miR-1224-5p, we constructed liver-specific adenoviral vectors expressing either an miR-1224-5p inhibitor sequence or miR-1224-5p mimic sequences. Following tail-vein vector injection, HFD-fed mice were examined for expression of lipogenic genes. We found that miR-1224-5p inhibitors significantly attenuated hepatic lipogenesis and steatosis in HFD-fed mice, while miR-1224-5p mimics promoted lipid accumulation in the liver of chow-fed C57BL/6 mice. Additional in vitro studies demonstrated that downregulation of miR-1224-5p in HepG2 and primary hepatocytes led to a reduction of cellular triglycerides following treatment with an oleic acid/palmitic acid mixture. Importantly, this study also identified adenosine monophosphate (AMP)-activated protein kinase (AMPK)-α1 as a direct target of miR-1224-5p. MiR-1224-5p binding to the 3'-UTR of AMPKα1 suppressed expression of the AMPKα1 protein and its downstream molecules. Metformin, an activator of AMPK, also inhibited hepatic expression of miR-1224-5p. Together, these findings indicate that miR-1224-5p promotes hepatic lipogenesis by suppressing AMPKα1 expression and suggest that miR-1224-5p inhibitors warrant further investigation as potential therapeutic tools in the treatment of non-alcoholic fatty liver disease.
2470 related Products with: MiR-1224-5p enhances hepatic lipogenesis by targeting Adenosine monophosphate activated protein kinase α1 in male mice.Activated Carrier Protei Activated Carrier Protei Activated Carrier Protei Activated Carrier Protei Activated Carrier Protei Activated Carrier Protei Activated Carrier Protei p130Cas-associated protei Activated Protein C Inact HIV 1 intergase antigen. Anti C Reactive Protein A Human Macrophage Inflamma
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Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection.The concerns raised from adenovirus 5 (Ad5)-based HIV vaccine clinical trials, where excess HIV infections were observed in some vaccine recipients, have highlighted the importance of understanding host responses to vaccine vectors and the HIV susceptibility of vector-specific CD4 T cells in HIV vaccination. Our recent study reported that human Ad5-specific CD4 T cells induced by Ad5 vaccination (RV156A trial) are susceptible to HIV. Here we further investigated the HIV susceptibility of vector-specific CD4 T cells induced by ALVAC, a canarypox viral vector tested in the Thai trial RV144, as compared to Ad5 vector-specific CD4 T cells in the HVTN204 trial. We showed that while Ad5 vector-specific CD4 T cells were readily susceptible to HIV, ALVAC-specific CD4 T cells in RV144 PBMC were substantially less susceptible to both R5 and X4 HIV in vitro. The lower HIV susceptibility of ALVAC-specific CD4 T cells was associated with the reduced surface expression of HIV entry co-receptors CCR5 and CXCR4 on these cells. Phenotypic analyses identified that ALVAC-specific CD4 T cells displayed a strong Th1 phenotype, producing higher levels of IFN-γ and CCL4 (MIP-1β) but little IL-17. Of interest, ALVAC and Ad5 vectors induced distinct profiles of vector-specific CD8 vs. CD4 T-cell proliferative responses in PBMC, with ALVAC preferentially inducing CD8 T-cell proliferation, while Ad5 vector induced CD4 T-cell proliferation. Depletion of ALVAC-, but not Ad5-, induced CD8 T cells in PBMC led to a modest increase in HIV infection of vector-specific CD4 T cells, suggesting a role of ALVAC-specific CD8 T cells in protecting ALVAC-specific CD4 T cells from HIV. Taken together, our data provide strong evidence for distinct HIV susceptibility of CD4 T cells induced by different vaccine vectors and highlight the importance of better evaluating anti-vector responses in HIV vaccination.
2099 related Products with: Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection.HIV type O envelope antig HIV 1 reverse transcripta HIV 1 Reverse transcripta HIV 1 Reverse transcripta HIV 1 Reverse transcripta HIV 1 Reverse transcripta HIV1 tat antibody, Monocl HIV1 tat antibody, Monocl HIV Self Test Kit, 1Test HIV I&II test strip, Infe Recombinant HIV Type-O En Recombinant HIV Type-O En
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Downregulated Nuclear Factor E2-Related Factor 2 (Nrf2) Aggravates Cognitive Impairments via Neuroinflammation and Synaptic Plasticity in the Senescence-Accelerated Mouse Prone 8 (SAMP8) Mouse: A Model of Accelerated Senescence.BACKGROUND We observed the effects of nuclear factor E2-related factor 2 (Nrf2) downregulation via intrahippocampal injection of a lentiviral vector on cognition in senescence-accelerated mouse prone 8 (SAMP8) to investigate the role of the (Nrf2)/antioxidant response element (ARE) pathway in age-related changes. MATERIAL AND METHODS Control lentivirus and Nrf2-shRNA-lentivirus were separately injected into the hippocampus of 4-month-old SAMR1 and SAMP8 mice and then successfully downregulated Nrf2 expression in this brain region. Five months later, cognitive function tests, including the novel object test, the Morris water maze test, and the passive avoidance task were conducted. Glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba1) immunohistochemistry was performed to observe an inflammatory response. Presynaptic synapsin (SYN) were observed by immunofluorescence. We then determined the Nrf2-regulated, heme oxygenase-1 (HO-1), P65, postsynaptic density protein (PSD), and SYN protein levels. The ultrastructure of neurons and synapses in the hippocampal CA1 region was observed by transmission electron microscopy. RESULTS Aging led to a decline in cognitive function compared with SAMR1 mice and the Nrf2-shRNA-lentivirus further exacerbated the cognitive impairment in SAMP8 mice. Nrf2, HO-1, PSD, and SYN levels were significantly reduced (all P<0.05) but high levels of inflammation were detected in SAMP8 mice with low expression of Nrf2. Furthermore, neurons were vacuolated, the number of organelles decreased, and the number of synapses decreased. CONCLUSIONS Downregulation of Nrf2 suppressed the Nrf2/ARE pathway, activated oxidative stress and neuroinflammation, and accelerated cognitive impairment in SAMP8 mice. Downregulation of Nrf2 accelerates the aging process through neuroinflammation and synaptic plasticity.
2663 related Products with: Downregulated Nuclear Factor E2-Related Factor 2 (Nrf2) Aggravates Cognitive Impairments via Neuroinflammation and Synaptic Plasticity in the Senescence-Accelerated Mouse Prone 8 (SAMP8) Mouse: A Model of Accelerated Senescence.Mouse Stromal Cell-Derive Mouse Platelet Derived Gr Mouse Anti-Insulin-Like G Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Hamster anti mouse Insuli Rat monoclonal anti mouse Rabbit Polyclonal Antibod
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[Prokaryotic expression and identification of rhoptry protein 38 of].To explore the biological function of rhoptry protein 38 (ROP38) of, and to identify the reactogenicity of the recombinant protein (rROP38).
baculoCOMPLETE protein ex ∆1-Androstene-3α,17β- Rabbit Anti-Rex1 Zinc fin Rabbit Anti-Rex1 Zinc fin Rabbit Anti-Rat Androgen AccuRapid™ Cell Free Pr Recombinant M. tuberculos Recombinant Human Androge Recombinant Mn SOD (Human Myelin Basic Protein Heat Shock Protein 70 (H Heat Shock Protein 70 (H
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[Cloning, expression and serological evaluation of H3 protein from].To clone and express the basement membrane specific heparan sulfate proteoglycan core protein (H3), and to evaluate its effect in detection of human cystic echinococcosis (CE).
baculoCOMPLETE protein ex Lentiviral Technology pCD Rabbit Anti-Rat Androgen pYLEX1 - Expression Vect AccuRapid™ Cell Free Pr Native Influenza HA (A Pa Native Influenza HA (A Wi Recombinant Human Androge Recombinant Mn SOD (Human Myelin Basic Protein Heat Shock Protein 70 (H Heat Shock Protein 70 (H
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[Cloning, fusion expression and identification of thioredoxin encoding gene from].To clone and express the thioredoxin (Trx) from RH strain tachyzoites of, establish the prokaryotic expression vector and purify the recombinant protein, then produce the polyclonal anti-Trx antibody in rabbits.
2717 related Products with: [Cloning, fusion expression and identification of thioredoxin encoding gene from].pYLEX1 - Expression Vect DNA (cytosine 5) methyltr Gene Expression: Mouse N Gene Expression: Rat P45 Lentiviral Technology pCD pCdgCAT Mammalian CAT Exp pCAMBIA0305.1 Vector, (Gu pCAMBIA0305.2 Vector (Sec pCAMBIA0380 Vector (No Re pCAMBIA1105.1 (GusPlus™ pCAMBIA1105.1R Vecotr (Gu pCAMBIA1200 Vector (No Re
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[Cloning and characterization of β-carbonic anhydrase, a potential drug target of].To express the beta carbonic anhydrase (β-CA) of, and analyze its catalytic activity.
2336 related Products with: [Cloning and characterization of β-carbonic anhydrase, a potential drug target of].Carbonic anhydrase 9 anti Mouse anti human Carbonic Rabbit Anti-Bovine Carbon Anti-CA8(Carbonic anhydra Anti CA8(Carbonic anhydra MOUSE ANTI BOVINE ROTAVIR Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge MOUSE ANTI BORRELIA BURGD Androgen Receptor (Ab 650
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Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation ofat its optimum growth temperature.is a leading candidate for the consolidated bioprocessing of lignocellulosic biomass for the production of fuels and chemicals. A limitation to the engineering of this strain is the availability of stable replicating plasmid vectors for homologous and heterologous expression of genes that provide improved and/or novel pathways for fuel production. Current vectors relay on replicons from mesophilic bacteria and are not stable at the optimum growth temperature of. To develop more thermostable genetic tools for, we constructed vectors based on the hyperthermophilicreplicon pBAS2. Autonomously replicating shuttle vectors based on pBAS2 reproducibly transformedat 60 °C and were maintained in multiple copy. Promoters, selectable markers and plasmid replication proteins fromwere functional in. Phylogenetic analyses of the proteins contained on pBAS2 revealed that the replication initiation protein RepL is unique among thermophiles. These results suggest that pBAS2 may be a broadly useful replicon for other thermophilic Firmicutes.
1105 related Products with: Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation ofat its optimum growth temperature.Growth Differentiation Fa IGF-1R Signaling Phospho- Growth Factor (Human) Ant Homogenizer for 24 sample Homogenizer for 8 samples Breast invasive ductal ca Goat Anti-Human Fibroblas Mouse Anti-Insulin-Like G Multiple lung carcinoma ( pCAMBIA0105.1R Vector, (G Rat monoclonal anti mouse Rat monoclonal anti mouse
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Stage-Dependent Expression of Deltamethrin Toxicity and Resistance in Triatoma infestans (Hemiptera: Reduviidae) From Argentina.Triatoma infestans Klug (Hemiptera: Reduviidae) is the main vector of Chagas disease in Latin America. This insect has been controlled with pyrethroids since the 1980s, although the emergence of resistance to deltamethrin has decreased control success in some areas of the Gran Chaco ecoregion. The response of T. infestans to deltamethrin was evaluated per developmental stage. In addition, we evaluated the possible stage-dependent expression of deltamethrin resistance. The bioassays were conducted by topical application of the insecticide in acetone. The drop size, age at the time of exposure, and mortality measuring time were standardized per stage. The lethal dose of deltamethrin moderately increased with the developmental stage. The resistance to deltamethrin was expressed in every instar, and was the highest in the fourth- and fifth-instar nymphs. While increasing, weight plays a relevant role in lethal dose stage dependency, a number of contributing factors such as degradative metabolism are probably involved in the variability of insecticide effect and resistance described for different T. infestans developmental stages. Possible explanations for these differences and their implications on resistance management and chemical control are discussed.
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