Search results for: miRNASelect™ pEGP-mmu-mir-684-2 Expression Vector
#38647896 
2022/11/17
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Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris.
CLingling Ma, Xiaolin Liang, Shiqin Yu, Jingwen Zhou
1149 related Products with: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris.
100ul10.00 ug2 20 10 100ug Lyophilized10 mg10 1mg2 2 2
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#38647895 2023/01/21
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Expression of recombinant human Apolipoprotein A-I in Nicotiana tabacum.
AWei Zhao, Lu-Yang Zhou, Jing Kong, Ze-Hao Huang, Ya-Di Gao, Zhong-Xia Zhang, Yong-Jie Zhou, Ruo-Yu Wu, Hong-Jun Xu, Sheng-Jun An
1682 related Products with: Expression of recombinant human Apolipoprotein A-I in Nicotiana tabacum.
10 25 mg2 2 10 25 10 5 10 100 mg1mg100
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#38647454 2024/04/22
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A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector.
Uncovering the function of phytopathogen effectors is crucial for understanding mechanisms of pathogen pathogenicity and for improving our ability to protect plants from diseases. An increasing number of effectors have been predicted in various plant pathogens. Functional characterization of these effectors has become a major focus in the study of plant-pathogen interactions. In this study, we designed a novel screening system that combines the TMV (tobacco mosaic virus)-GFP vector and Agrobacterium-mediated transient expression in the model plant Nicotiana benthamiana. This system enables the rapid identification of effectors that interfere with plant immunity. The biological function of these effectors can be easily evaluated by observing the GFP fluorescence signal using a UV lamp within just a few days. To evaluate the TMV-GFP system, we initially tested it with well-described virulence and avirulence type III effectors from the bacterial pathogen Ralstonia solanacearum. After proving the accuracy and efficiency of the TMV-GFP system, we successfully screened a novel virulence effector, RipS1, using this approach. Furthermore, using the TMV-GFP system, we reproduced consistent results with previously known cytoplasmic effectors from a diverse array of pathogens. Additionally, we demonstrated the effectiveness of the TMV-GFP system in identifying apoplastic effectors. The easy operation, time-saving nature, broad effectiveness, and low technical requirements of the TMV-GFP system make it a promising approach for high-throughput screening of effectors with immune interference activity from various pathogens.Peng Cao, Haotian Shi, Shuangxi Zhang, Jialan Chen, Rongbo Wang, Peiqing Liu, Yingfang Zhu, Yuyan An, Meixiang Zhang
2083 related Products with: A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector.
400Tests4 Sample Kit100 assays100 assays100 tests1 kit(96 Wells)100 assays40 assays1 kit(96 Wells)100 assays4 Sample Kit
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#38646789 2024/04/22
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Sodium-glucose co-transporter 1 promotes the bio-functions of perivascular preadipocytes mediated by Akt/mTOR/p70S6K signaling pathway.
The influence of SGLT-1 on perivascular preadipocytes (PVPACs) and vascular remodeling is not well understood. This study aimed to elucidate the role and mechanism of SGLT-1-mediated PVPACs bioactivity. PVPACs were cultured and applied to the carotid arteries of mice using a lentivirus-based thermosensitive in situ gel (TISG). The groups were treated with Lv-SGLT1 (lentiviral vector, overexpression), Lv-siSGLT1 (RNA interference, knockdown), or specific signaling pathway inhibitors. Assays were conducted to assess changes in cell proliferation, apoptosis, glucose uptake, adipogenic differentiation, and vascular remodeling in the PVPACs. Protein expression was analyzed by western blotting, immunocytochemistry, and/or immunohistochemistry. The methyl thiazolyl tetrazolium (MTT) assay and Hoechst 33342 staining indicated that SGLT-1 overexpression significantly promoted PVPACs proliferation and inhibited apoptosis . Conversely, SGLT-1 knockdown exerted the opposite effect. Oil Red O staining revealed that SGLT-1 overexpression facilitated adipogenic differentiation, while its inhibition mitigated these effects. H-labeled glucose uptake experiments demonstrated that SGLT-1 overexpression enhanced glucose uptake by PVPACs, whereas RNA interference-mediated SGLT-1 inhibition had no significant effect on glucose uptake. Moreover, RT-qPCR, western blotting, and immunofluorescence analyses revealed that SGLT-1 overexpression upregulated FABP4 and VEGF-A levels and activated the Akt/mTOR/p70S6K signaling pathway, whereas SGLT-1 knockdown produced the opposite effects. studies corroborated these findings and indicated that SGLT-1 overexpression facilitated carotid artery remodeling. Our study demonstrates that SGLT-1 activation of the Akt/mTOR/p70S6K signaling pathway promotes PVPACs proliferation, adipogenesis, glucose uptake, glucolipid metabolism, and vascular remodeling.Zhiquan Liu, Jiayu Wang, Peiqing Tian, Yixuan Liu, Liyun Xing, Caihua Fu, Xianwei Huang, Ping Liu
1304 related Products with: Sodium-glucose co-transporter 1 promotes the bio-functions of perivascular preadipocytes mediated by Akt/mTOR/p70S6K signaling pathway.
100ug100ug100ug2 Pieces/Box100ug 100 G2.5 mg100 ml100 MG100ug250ML10ml
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#38646644 2024/03/25
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Hepatic progenitor cell-originated ductular reaction facilitates liver fibrosis through activation of hedgehog signaling.
It is poorly understood what cellular types participate in ductular reaction (DR) and whether DR facilitates recovery from injury or accelerates hepatic fibrosis. The aim of this study is to gain insights into the role of hepatic progenitor cell (HPC)-originated DR during fibrotic progression. DR in liver specimens of PBC, chronic HBV infection (CHB) or NAFLD, and four rodent fibrotic models by different pathogenic processes was evaluated. Gli1 expression was inhibited in rodent models or cell culture and organoid models by AAV-sh or treating with GANT61. Severity of liver fibrosis was positively correlated with DR extent in patients with PBC, CHB or NAFLD. HPCs were activated, expanded, differentiated into reactive cholangiocytes and constituted "HPC-originated DR", accompanying with exacerbated fibrosis in rodent models of HPC activation & proliferation (CCl/2-AAF-treated), spontaneous PSC, BDL-cholestatic fibrosis or WD-fed/CCl-treated NASH-fibrosis. Gli1 expression was significantly increased in enriched pathways and . Enhanced Gli1 expression was identified in KRT19-reactive cholangiocytes. Suppressing Gli1 expression by administration of AAV-sh or GANT61 ameliorated HPC-originated DR and fibrotic extent. KRT19 expression was reduced after GANT61 treatment in sodium butyrate-stimulated WB-F344 cells or organoids or in cells transduced with Gli1 knockdown lentiviral vectors. In contrast, KRT19 expression was elevated after transducing Gli1 overexpression lentiviral vectors in these cells. During various modes of chronic injury, Gli1 acted as an important mediator of HPC activation, expansion, differentiation into reactive cholangiocytes that formed DR, and subsequently provoked hepatic fibrogenesis.Yonghong Hu, Xinyu Bao, Zheng Zhang, Long Chen, Yue Liang, Yan Qu, Qun Zhou, Xiaoxi Zhou, Jing Fang, Zhun Xiao, Yadong Fu, Hailin Yang, Wei Liu, Ying Lv, Hongyan Cao, Gaofeng Chen, Jian Ping, Hua Zhang, Yongping Mu, Chenghai Liu, Chao-Po Lin, Jian Wu, Ping Liu, Jiamei Chen
2364 related Products with: Hepatic progenitor cell-originated ductular reaction facilitates liver fibrosis through activation of hedgehog signaling.
500 reactions1.5x10(6) cells24 reactions 4Inhibitors1.5 x 10^6 cells2 Pieces/Box6
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