Gentaur Pub

#38630832   2024/04/17 To Up

Perceived needs of disease vector control programs: A review and synthesis of (sub)national assessments from South Asia and the Middle East.

Systems for disease vector control should be effective, efficient, and flexible to be able to tackle contemporary challenges and threats in the control and elimination of vector-borne diseases. As a priority activity towards the strengthening of vector control systems, it has been advocated that countries conduct a vector-control needs assessment. A review was carried out of the perceived needs for disease vector control programs among eleven countries and subnational states in South Asia and the Middle East. In each country or state, independent teams conducted vector control needs assessment with engagement of stakeholders. Important weaknesses were described for malaria, dengue and leishmaniases regarding vector surveillance, insecticide susceptibility testing, monitoring and evaluation of operations, entomological capacity and laboratory infrastructure. In addition, community mobilization and intersectoral collaboration showed important gaps. Countries and states expressed concern about insecticide resistance that could reduce the continued effectiveness of interventions, which demands improved monitoring. Moreover, attainment of disease elimination necessitates enhanced vector surveillance. Vector control needs assessment provided a useful planning tool for systematic strengthening of vector control systems. A limitation in conducting the vector control needs assessment was that it is time- and resource-intensive. To increase the feasibility and utility of national assessments, an abridged version of the guidance should focus on operationally relevant topics of the assessment. Similar reviews are needed in other regions with different contextual conditions.
Henk van den Berg, Kabirul Bashar, Rajib Chowdhury, Rajendra M Bhatt, Hardev Prasad Gupta, Ashwani Kumar, Shanmugavelu Sabesan, Ananganallur N Shriram, Hari Kishan Raju Konuganti, Akhouri T S Sinha, Mohammad Mehdi Sedaghat, Ahmadali Enayati, Hameeda Mohammed Hassan, Aishath Shaheen Najmee, Sana Saleem, Surendra Uranw, Pahalagedera H D Kusumawathie, Devika Perera, Mohammed A Esmail, Lauren B Carrington, Samira M Al-Eryani, Roop Kumari, Bhupender N Nagpal, Sabera Sultana, Raman Velayudhan, Rajpal S Yadav

1487 related Products with: Perceived needs of disease vector control programs: A review and synthesis of (sub)national assessments from South Asia and the Middle East.

100ug1000 tests25 mg5mg100ul2.5 mg10 mg100ul 5 G100ug1,000 tests

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#38630231   // To Up

Live Imaging of Migrating Neurons and Glial Progenitors Visualized by in Utero Electroporation.

During cortical development, both neurons and glial cells are generated in the germinal zone near the lateral ventricle, migrate in the correct direction, and settle in their appropriate locations. This developmental process can be clearly visualized by introducing fluorescent protein-expression vectors via in utero electroporation. In this chapter, we describe labeling methods for migrating neurons and glial progenitors, as well as methods for slice culture, and time-lapse imaging.
Masashi Nishikawa, Koh-Ichi Nagata, Hidenori Tabata

2304 related Products with: Live Imaging of Migrating Neurons and Glial Progenitors Visualized by in Utero Electroporation.

900 tests

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#38629194   // To Up

Chinese cherry CpMYB44-CpSPDS2 module regulates spermidine content and florescence in tobacco.

The flower bud differentiation plays a crucial role in cherry yield and quality. In a preliminary study, we revealed the promotion of spermidine (Spd) in bud differentiation and quality. However, the molecular mechanism underlying Spd regulating cherry bud differentiation remains unclear. To address this research gap, we cloned CpSPDS2, a gene that encodes Spd synthase and is highly expressed in whole flowers and pistils of the Chinese cherry (cv. 'Manaohong'). Furthermore, an overexpression vector with this gene was constructed to transform tobacco plants. The findings demonstrated that transgenic lines exhibited higher Spd content, an earlier flowering time by 6 d, and more lateral buds and flowers than wild-type lines. Additionally, yeast one-hybrid assays and two-luciferase experiments confirmed that the R2R3-MYB transcription factor (CpMYB44) directly binds to and activates the CpSPDS2 promoter transcription. It is indicated that CpMYB44 promotes Spd accumulation via regulating CpSPDS2 expression, thus accelerating the flower growth. This research provides a basis for resolving the molecular mechanism of CpSPDS2 involved in cherry bud differentiation.
Hong Deng, Qiandong Hou, Zhuang Wen, Runrun Yu, Xuejiao Cao, Chunqiong Shang, Xiaowei Cai, Guang Qiao

1739 related Products with: Chinese cherry CpMYB44-CpSPDS2 module regulates spermidine content and florescence in tobacco.

280025 1600100 μg100 μg100 μg20 10 mg

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#38627969   2024/04/15 To Up

Evolution of the clinical-stage hyperactive TcBuster transposase as a platform for robust non-viral production of adoptive cellular therapies.

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Joseph G Skeate, Emily J Pomeroy, Nicholas J Slipek, Bryan J Jones, Bryce J Wick, Jae-Woong Chang, Walker S Lahr, Erin M Stelljes, Xiaobai Patrinostro, Blake Barnes, Trevor Zarecki, Joshua B Krueger, Jacob E Bridge, Gabrielle M Robbins, Madeline D McCormick, John R Leerar, Kari T Wenzel, Kathlyn M Hornberger, Kirsti Walker, Dalton Smedley, David A Largaespada, Neil Otto, Beau R Webber, Branden S Moriarity

1670 related Products with: Evolution of the clinical-stage hyperactive TcBuster transposase as a platform for robust non-viral production of adoptive cellular therapies.

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#38626865   2024/04/14 To Up

Pathogen-associated molecular patterns (PAMPs) derived from Leishmania and bacteria increase gene expression of antimicrobial peptides and gut surface proteins in sand flies.

The interaction between pathogens and vectors' physiology can impact parasite transmission. Studying this interaction at the molecular level can help in developing control strategies. We study leishmaniases, diseases caused by Leishmania parasites transmitted by sand fly vectors, posing a significant global public health concern. Lipophosphoglycan (LPG), the major surface glycoconjugate of Leishmania, has been described to have several roles throughout the parasite's life cycle, both in the insect and vertebrate hosts. In addition, the sand fly midgut possesses a rich microbiota expressing lipopolysaccharides (LPS). However, the effect of LPG and LPS on the gene expression of sand fly midgut proteins or immunity effectors has not yet been documented. We experimentally fed Lutzomyia longipalpis and Phlebotomus papatasi sand flies with blood containing purified LPG from Leishmania infantum, Leishmania major, or LPS from Escherichia coli. The effect on the expression of genes encoding gut proteins galectin and mucin, digestive enzymes trypsin and chymotrypsin, and antimicrobial peptides (AMPs) attacin and defensins was assessed by quantitative PCR (qPCR). The gene expression of a mucin-like protein in L. longipalpis was increased by L. infantum LPG and E. coli LPS. The gene expression of a galectin was increased in L. longipalpis by L. major LPG, and in P. papatasi by E. coli LPS. Nevertheless, the gene expression of trypsins and chymotrypsins did not significantly change. On the other hand, both L. infantum and L. major LPG significantly enhanced expression of the AMP attacin in both sand fly species and defensin in L. longipalpis. In addition, E. coli LPS increased the expression of attacin and defensin in L. longipalpis. Our study showed that Leishmania LPG and E. coli LPS differentially modulate the expression of sand fly genes involved in gut maintenance and defence. This suggests that the glycoconjugates from microbiota or Leishmania may increase the vector's immune response and the gene expression of a gut coating protein in a permissive vector.
Barbora Vomáčková Kykalová, Fabiana Sassù, Felipe Dutra Rêgo, Rodrigo Pedro Soares, Petr Volf, Erich Loza Telleria

2466 related Products with: Pathogen-associated molecular patterns (PAMPs) derived from Leishmania and bacteria increase gene expression of antimicrobial peptides and gut surface proteins in sand flies.

100ul300 units200ul1005mg

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#38626731   2024/04/16 To Up

Forward problem of electrocardiography based on cardiac source vector orientations.

To localize the unusual cardiac activities non-invasively, one has to build a prior forward model that relates the heart, torso, and detectors. This model has to be constructed to mathematically relate the geometrical and functional activities of the heart. Several methods are available to model the prior sources in the forward problem, which results in the lead field matrix generation. In the conventional technique, the lead field assumed the fixed prior sources, and the source vector orientations were presumed to be parallel to the detector plane with the unit strength in all directions. However, the anomalies cannot always be expected to occur in the same location and orientation, leading to misinterpretation and misdiagnosis. To overcome this, the work proposes a new forward model constructed using the VCG signals of the same subject. Furthermore, three transformation methods were used to extract VCG in constructing the time-varying lead field to steer to the orientation of the source rather than just reconstructing its activities in the inverse problem. In addition, the unit VCG loop of the acute ischemia patient was extracted to observe the changes compared to the normal subject. The abnormality condition was achieved by reducing the depolarization time by 15ms. The results involving the unit vectors of VCG demonstrated the anisotropic nature of cardiac source orientations, providing information about the heart's electrical activity.
Reshma H, Vikas R Bhat, Anitha H

2102 related Products with: Forward problem of electrocardiography based on cardiac source vector orientations.

100ul 100ul 100ul 100ul1mg 100ul 100ul100ug1 mg50ul 100ul 100ul

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#38626531   2024/04/09 To Up

Overexpression of Slc22a18 facilitates fat accumulation in mice.

We previously reported that solute carrier family 22 member 18 (Slc22a18) regulates lipid accumulation in 3T3-L1 adipocytes. Here, we provide additional evidence derived from experiments with adenoviral vector expression and genetic manipulation of mice. In primary cultured rat hepatocytes, adenoviral overexpression of mouse Slc22a18 increased triglyceride accumulation and triglyceride synthetic activity, which was decreased in an adenoviral knockdown experiment. Adenoviral overexpression of mouse Slc22a18 in vivo caused massive fatty liver in mice, even under normal dietary conditions. Conversely, adenoviral knockdown of mouse Slc22a18 reduced hepatic lipid accumulation induced by a high-glucose and high-sucrose diet. We created Slc22a18 knockout mice, which grew normally and showed no obvious spontaneous phenotypes. However, compared with control littermates, the knockout mice exhibited decreased hepatic triglyceride content under refeeding conditions, significantly reduced epididymal fat mass, and tended to have lower liver weight in conjunction with leptin deficiency. Finally, we created transgenic mice overexpressing rat Slc22a18 in an adipose-specific manner, which had increased body weight and epididymal fat mass primarily because of increased adipocyte cell volume. In these transgenic mice, a positive correlation was observed between adiposity and the expression levels of the rat Slc22a18 transgene. Taken together, these results indicate that Slc22a18 has positive effects on lipid accumulation in vivo.
Takashi Yamamoto, Yoko Iizuka, Kozue Izumi-Yamamoto, Midori Shirota, Nobuko Mori, Yoshikazu Tahara, Toshiro Fujita, Takanari Gotoda

1425 related Products with: Overexpression of Slc22a18 facilitates fat accumulation in mice.

3 inhibitors96 tests96tests1 Set1 Set1 g1 Set 25 G1 Set1 mg

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#38626311   2024/04/16 To Up

ATP13A3 variants promote pulmonary arterial hypertension by disrupting polyamine transport.

Potential loss-of-function variants of ATP13A3, the gene encoding a P5B-type transport ATPase of undefined function, were recently identified in pulmonary arterial hypertension (PAH) patients. ATP13A3 is implicated in polyamine transport but its function has not been fully elucidated. Here, we sought to determine the biological function of ATP13A3 in vascular endothelial cells and how PAH-associated variants may contribute to disease pathogenesis.
Bin Liu, Mujahid Azfar, Ekaterina Legchenko, James A West, Shaun Martin, Chris Van den Haute, Veerle Baekelandt, John Wharton, Luke Howard, Martin R Wilkins, Peter Vangheluwe, Nicholas W Morrell, Paul D Upton

2755 related Products with: ATP13A3 variants promote pulmonary arterial hypertension by disrupting polyamine transport.

500 ml 1000 ml 100ug 500 ml 100ul 25UG 1000 ml 20 Bags of 25 Tubes/Unit5 x 200ul 500 ml

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#38626093   2024/04/16 To Up

Peak transgene expression after intramuscular immunization of mice with adenovirus 26-based vector vaccines correlates with transgene-specific adaptive immune responses.

Non-replicating adenovirus-based vectors have been broadly used for the development of prophylactic vaccines in humans and are licensed for COVID-19 and Ebola virus disease prevention. Adenovirus-based vectored vaccines encode for one or more disease specific transgenes with the aim to induce protective immunity against the target disease. The magnitude and duration of transgene expression of adenovirus 5- based vectors (human type C) in the host are key factors influencing antigen presentation and adaptive immune responses. Here we characterize the magnitude, duration, and organ biodistribution of transgene expression after single intramuscular administration of adenovirus 26-based vector vaccines in mice and evaluate the differences with adenovirus 5-based vector vaccine to understand if this is universally applicable across serotypes. We demonstrate a correlation between peak transgene expression early after adenovirus 26-based vaccination and transgene-specific cellular and humoral immune responses for a model antigen and SARS-CoV-2 spike protein, independent of innate immune activation. Notably, the memory immune response was similar in mice immunized with adenovirus 26-based vaccine and adenovirus 5-based vaccine, despite the latter inducing a higher peak of transgene expression early after immunization and a longer duration of transgene expression. Together these results provide further insights into the mode of action of adenovirus 26-based vector vaccines.
Sonia Marquez-Martinez, Nadine Salisch, Jan Serroyen, Roland Zahn, Selina Khan

2212 related Products with: Peak transgene expression after intramuscular immunization of mice with adenovirus 26-based vector vaccines correlates with transgene-specific adaptive immune responses.

100 µL

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#38626020   2024/04/16 To Up

Members of the paralogous gene family 12 from the Lyme disease agent Borrelia burgdorferi are non-specific DNA-binding proteins.

Lyme disease is the most prevalent vector-borne infectious disease in Europe and the USA. Borrelia burgdorferi, as the causative agent of Lyme disease, is transmitted to the mammalian host during the tick blood meal. To adapt to the different encountered environments, Borrelia has adjusted the expression pattern of various, mostly outer surface proteins. The function of most B. burgdorferi outer surface proteins remains unknown. We determined the crystal structure of a previously uncharacterized B. burgdorferi outer surface protein BBK01, known to belong to the paralogous gene family 12 (PFam12) as one of its five members. PFam12 members are shown to be upregulated as the tick starts its blood meal. Structural analysis of BBK01 revealed similarity to the coiled coil domain of structural maintenance of chromosomes (SMC) protein family members, while functional studies indicated that all PFam12 members are non-specific DNA-binding proteins. The residues involved in DNA binding were identified and probed by site-directed mutagenesis. The combination of SMC-like proteins being attached to the outer membrane and exposed to the environment or located in the periplasm, as observed in the case of PFam12 members, and displaying the ability to bind DNA, represents a unique feature previously not observed in bacteria.
Kalvis Brangulis, Inara Akopjana, Laura Drunka, Sofija Matisone, Diana Zelencova-Gopejenko, Shapla Bhattacharya, Janis Bogans, Kaspars Tars

2921 related Products with: Members of the paralogous gene family 12 from the Lyme disease agent Borrelia burgdorferi are non-specific DNA-binding proteins.

100 UG1 mL100μl1mg1 mL100μl2 Pieces/Box101 mL100μl1 mg100μl

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