Gentaur Pub

#38647896   2022/11/17 To Up

Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris.

C
Lingling Ma, Xiaolin Liang, Shiqin Yu, Jingwen Zhou

1308 related Products with: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris.

2 10.00 ug20 10 100ug Lyophilized 100ul2 2 10 mg10 1mg50

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#38647895   2023/01/21 To Up

Expression of recombinant human Apolipoprotein A-I in Nicotiana tabacum.

A
Wei Zhao, Lu-Yang Zhou, Jing Kong, Ze-Hao Huang, Ya-Di Gao, Zhong-Xia Zhang, Yong-Jie Zhou, Ruo-Yu Wu, Hong-Jun Xu, Sheng-Jun An

2339 related Products with: Expression of recombinant human Apolipoprotein A-I in Nicotiana tabacum.

10 25 10 5 10 25 mg2 2 1mg50 10 100 mg

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#38647454   2024/04/22 To Up

A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector.

Uncovering the function of phytopathogen effectors is crucial for understanding mechanisms of pathogen pathogenicity and for improving our ability to protect plants from diseases. An increasing number of effectors have been predicted in various plant pathogens. Functional characterization of these effectors has become a major focus in the study of plant-pathogen interactions. In this study, we designed a novel screening system that combines the TMV (tobacco mosaic virus)-GFP vector and Agrobacterium-mediated transient expression in the model plant Nicotiana benthamiana. This system enables the rapid identification of effectors that interfere with plant immunity. The biological function of these effectors can be easily evaluated by observing the GFP fluorescence signal using a UV lamp within just a few days. To evaluate the TMV-GFP system, we initially tested it with well-described virulence and avirulence type III effectors from the bacterial pathogen Ralstonia solanacearum. After proving the accuracy and efficiency of the TMV-GFP system, we successfully screened a novel virulence effector, RipS1, using this approach. Furthermore, using the TMV-GFP system, we reproduced consistent results with previously known cytoplasmic effectors from a diverse array of pathogens. Additionally, we demonstrated the effectiveness of the TMV-GFP system in identifying apoplastic effectors. The easy operation, time-saving nature, broad effectiveness, and low technical requirements of the TMV-GFP system make it a promising approach for high-throughput screening of effectors with immune interference activity from various pathogens.
Peng Cao, Haotian Shi, Shuangxi Zhang, Jialan Chen, Rongbo Wang, Peiqing Liu, Yingfang Zhu, Yuyan An, Meixiang Zhang

1042 related Products with: A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector.

400Tests4 Sample Kit100 assays100 assays100 tests1 kit(96 Wells)100 assays40 assays1 kit(96 Wells)100 assays4 Sample Kit

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#38646789   2024/04/22 To Up

Sodium-glucose co-transporter 1 promotes the bio-functions of perivascular preadipocytes mediated by Akt/mTOR/p70S6K signaling pathway.

The influence of SGLT-1 on perivascular preadipocytes (PVPACs) and vascular remodeling is not well understood. This study aimed to elucidate the role and mechanism of SGLT-1-mediated PVPACs bioactivity. PVPACs were cultured and applied to the carotid arteries of mice using a lentivirus-based thermosensitive in situ gel (TISG). The groups were treated with Lv-SGLT1 (lentiviral vector, overexpression), Lv-siSGLT1 (RNA interference, knockdown), or specific signaling pathway inhibitors. Assays were conducted to assess changes in cell proliferation, apoptosis, glucose uptake, adipogenic differentiation, and vascular remodeling in the PVPACs. Protein expression was analyzed by western blotting, immunocytochemistry, and/or immunohistochemistry. The methyl thiazolyl tetrazolium (MTT) assay and Hoechst 33342 staining indicated that SGLT-1 overexpression significantly promoted PVPACs proliferation and inhibited apoptosis . Conversely, SGLT-1 knockdown exerted the opposite effect. Oil Red O staining revealed that SGLT-1 overexpression facilitated adipogenic differentiation, while its inhibition mitigated these effects. H-labeled glucose uptake experiments demonstrated that SGLT-1 overexpression enhanced glucose uptake by PVPACs, whereas RNA interference-mediated SGLT-1 inhibition had no significant effect on glucose uptake. Moreover, RT-qPCR, western blotting, and immunofluorescence analyses revealed that SGLT-1 overexpression upregulated FABP4 and VEGF-A levels and activated the Akt/mTOR/p70S6K signaling pathway, whereas SGLT-1 knockdown produced the opposite effects. studies corroborated these findings and indicated that SGLT-1 overexpression facilitated carotid artery remodeling. Our study demonstrates that SGLT-1 activation of the Akt/mTOR/p70S6K signaling pathway promotes PVPACs proliferation, adipogenesis, glucose uptake, glucolipid metabolism, and vascular remodeling.
Zhiquan Liu, Jiayu Wang, Peiqing Tian, Yixuan Liu, Liyun Xing, Caihua Fu, Xianwei Huang, Ping Liu

2660 related Products with: Sodium-glucose co-transporter 1 promotes the bio-functions of perivascular preadipocytes mediated by Akt/mTOR/p70S6K signaling pathway.

100ug100ug2 Pieces/Box100ug 100 G2.5 mg100 ml100 MG100ug2 Pieces/Box100ug1 mg

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#38645872   // To Up

[Prokaryotic Expression and Bioinformatic Analysis of Rv3432c From ].

To express the protein enconded by the 3432 gene of (.) by prokaryotic expression, to analyze the structure of the Rv3432c protein by using bioinformatics software, and to explore for new drug targets against ..
Haibo Yi, Xinghong Gao, Guo Luo, Peng Xu, Huan Wang

2693 related Products with: [Prokaryotic Expression and Bioinformatic Analysis of Rv3432c From ].

1 module1000 tests 15 ml >1 x 10^6 IFUs1 module50 ug 100ug1 module10 ug

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#38645870   // To Up

[Immunoadjuvant Effect of Chitosan Oligosaccharide and Its Feasibility of Being Used as an Adjuvant for Attenuated Live Bacteria Vector Vaccines].

To study the immunoadjuvant effects of chitosan oligosaccharide (COS), including the immune activation and the triggering of lysosomal escape, and to explore whether COS can be used as an adjuvant for attenuated live bacteria vector vaccines.
Qian Ou, Zhaobin Chen, Jing Tang, Mengdie Chen, Yunwen Zhang, Zinan Chen, Qu Liu, Junrong Luo, Chuan Wang

2512 related Products with: [Immunoadjuvant Effect of Chitosan Oligosaccharide and Its Feasibility of Being Used as an Adjuvant for Attenuated Live Bacteria Vector Vaccines].

1 KIT10.2 mg96 Tests900 tests50 assays100 μg100μg 5 G100ug

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#38644543   2024/04/21 To Up

Establishment of a diagnostic model of endometriosis based on disulfidptosis-related genes.

We aimed to establish a diagnostic model of endometriosis (EM) based on disulfidptosis-related genes (DRGs).
Hongyan Shi, Caixia Zhou, Yaoyao Zhao

1875 related Products with: Establishment of a diagnostic model of endometriosis based on disulfidptosis-related genes.

100.00 ug1 mL 6 ml Ready-to-use 100ug2 Pieces/Box100ul100.00 ug 100ul0.1 mg

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