Search results for: anti_Mouse IgG h+l adsorbed with Rat IgG
#21703845 2011/06/06 To Up
Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor.
Using long-period gratings (LPG) inscribed in photonic crystal fiber (PCF) and coupling this structure with an optically aligned flow cell, we have developed an optofluidic refractive index transduction platform for label-free biosensing. The LPG-PCF scheme possesses extremely high sensitivity to the change in refractive index induced by localized binding event in different solution media. A model immunoassay experiment was carried out inside the air channels of PCF by a series of surface modification steps in sequence that include adsorption of poly(allylamine hydrochloride) monolayer, immobilization of anti-rat bone sialoprotein monoclonal primary antibody, and binding interactions with non-specific goat anti-rabbit IgG (H+L) and specific secondary goat anti-mouse IgG (H+L) antibodies. These adsorption and binding events were monitored in situ using the LPG-PCF by measuring the shift of the core-to-cladding mode coupling resonance wavelength. Steady and significant resonance changes, about 0.75 nm per nanometer-thick adsorbed/bound bio-molecules, have been observed following the sequence of the surface events with monolayer sensitivity, suggesting the promising potential of LPG-PCF for biological sensing and evaluation.Zonghu He, Fei Tian, Yinian Zhu, Nina Lavlinskaia, Henry Du
1844 related Products with: Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor.
100 μg48 samples900 tests0.1ml (1mg/ml)100 μg100 μg250 1 mg100 μg100 μg96 tests100 μgRelated Pathways
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