Search results for: anti_Dog C3
#20234115 
2010/03/16
To Up
Preparation of positive control platelets for detection of canine platelet surface-associated IgG, IgM and complement (C3) by flow cytometry.
An assay for detection of platelet surface-associated (PSA-) IgG, IgM and/or complement (C3) in dogs was modified by preparation of artificial positive control platelets. Flow cytometry of fluorescein isothiocyanate (FITC)-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 antibodies was used to detect the PSA proteins. IgM single, IgM/C3 double and IgG/IgM/C3 triple positive platelets were prepared. FITC-conjugated anti-IgG antibody bound strongly only to the triple positive platelets. Binding of FITC-conjugated anti-IgM or anti-C3 antibody to the double and triple positive platelets was specifically blocked by preincubation with the respective non-FITC-conjugated same-origin antibodies. These results confirm that FITC-conjugated antibodies specifically detect PSA proteins and that the control platelets prepared in this study are appropriate positive controls for detection of PSA proteins by flow cytometry.Ryo Tsuchiya, Takahiro Komatsu, Takefumi Ishikawa, Sakurako Neo, Mary F McConnell, Masaharu Hisasue, Takatsugu Yamada
1839 related Products with: Preparation of positive control platelets for detection of canine platelet surface-associated IgG, IgM and complement (C3) by flow cytometry.
5ml1 kit5.00 nmol100 ml1 kit10ml20 μg1 kit10.00 nmol1Unit
Related Pathways
#18844824 //
To Up
Pattern of Coombs' test reactivity has diagnostic significance in dogs with immune-mediated haemolytic anaemia.
To investigate the clinical significance of the pattern of Coombs' test reactivity in dogs with immune-mediated haemolytic anaemia.S M Warman, J K Murray, A Ridyard, J Eastwood, S Silva, M J Day
2614 related Products with: Pattern of Coombs' test reactivity has diagnostic significance in dogs with immune-mediated haemolytic anaemia.
20 ul24 tests100ug96 tests
Related Pathways
#8212520 //
To Up
Immunohistological observations on pulmonary tissues from dogs infected with Dirofilaria immitis.
Pulmonary tissues from non-infected dogs, naturally Dirofilaria immitis-infected dogs and experimentally infected puppies, selectively necropsied after infection, were assessed using peroxidase-antiperoxidase (PAP) staining technology. Sequential sections of pulmonary tissue were PAP stained with anti-fresh D. immitis serum, anti-paraffin processed D. immitis serum, anti-dog immunoglobulin (IgG, IgG Fc, IgM) sera and anti-dog complement (C3) serum, and examined for antibody, complement and for D. immitis antigen. The extent of alveolar septal thickening was positively correlated with infection status. Cellular infiltration was most evident surrounding obstructed areas where D. immitis were in situ. Antigenic material (microfilariae, eggs, fragmented filariae) labelled by PAP was identified in the pulmonary arteries, alveolar capillaries and alveolar septa. Deposits of complement and IgG, presumably associated with immune complex formation, were also observed in some of the infected dogs. Identification of antigen, antibodies and complement associated with alveolar septal pathology suggested that immune mechanisms were active in its development.K I Tanaka, R B Atwell
1760 related Products with: Immunohistological observations on pulmonary tissues from dogs infected with Dirofilaria immitis.
6 ml Ready-to-use 10 μg1 mg
Related Pathways
#2047590 //
To Up
Immunoperoxidase staining characteristics of Dirofilaria immitis in the dog.
The immunoperoxidase staining characteristics of Dirofilaria immitis and pulmonary tissues from infected dogs were studied by using the following sera: anti-fresh D immitis, anti-processed D immitis, anti-dog IgG, anti-dog IgG Fc, anti-dog IgM and anti-dog C3. Marked staining was observed using anti-fresh D immitis serum. Body cavity fluid and cuticle were strongly stained and hypodermis, muscle, lateral cord, testis, vas deferens, ovary, oviduct and uterus were moderately stained. Oesophagus and intestine were mildly stained. Degenerate worms were stained by all antisera. The intact and cut surfaces of microfilariae and eggs and sperm present in filariae were stained, but not their internal contents. Circulating and stored immotile microfilariae did not stain. Excreted eggs, presumed to be unfertilized and, or, degenerate, stained positively. Immunoperoxidase staining of routinely processed histological samples provides a means of assessing D immitis antigen.K I Tanaka, R B Atwell