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#34835439 
2021/11/08
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Chromium Stress Tolerance of a C4 ( L.) and C3 ( L.) Plants Primed with UV and Gamma-Treated .
Chromium stress is one of the deleterious abiotic factors that reduce crop production. Two anatomically different crops (C3 and C4) were compared for their chromium (0 and 50 ppm) tolerance and responses towards (). Strains of were exposed to UV (30-210 min) and gamma irradiation (1-4 KGy), and the best mutants were selected on petri plates containing selective markers. Maize and mungbean were supplied with selected strains or the parent strain in rooting medium, along with a nutrient broth. A completely randomized design (five replicates) was adopted using nutrient broth as a control. Stress negatively affected plants grown without strains. Mungbean was more sensitive towards stress and treatments, maize had better root and shoot fresh weights, root and shoot lengths, proline levels, and MDA and GR activity. All strains of (parent, γ-irradiated and UV-irradiated) enhanced proline, total soluble protein, chlorophyll a, a + b and a/b levels, with negligible effects upon antioxidant enzymes. Irradiated strains proved their superiority to the parent strain, with reductions in HO and MDA content. With comparable benefits, γ and UV irradiation may be adopted in future based upon technical availability.Qasim Shahzad, Saqib Mahmood, Sadia Javed, Tariq Mushtaq
1641 related Products with: Chromium Stress Tolerance of a C4 ( L.) and C3 ( L.) Plants Primed with UV and Gamma-Treated .
100ul100ul1 g96 wells (1 kit)100 mg50 ug 200ul10 mg100ug 25 MG100ug25 mg
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#29725025 2018/05/03
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Single vector platform vaccine protects against lethal respiratory challenge with Tier 1 select agents of anthrax, plague, and tularemia.
Bacillus anthracis, Yersinia pestis, and Francisella tularensis are the causative agents of Tier 1 Select Agents anthrax, plague, and tularemia, respectively. Currently, there are no licensed vaccines against plague and tularemia and the licensed anthrax vaccine is suboptimal. Here we report F. tularensis LVS ΔcapB (Live Vaccine Strain with a deletion in capB)- and attenuated multi-deletional Listeria monocytogenes (Lm)-vectored vaccines against all three aforementioned pathogens. We show that LVS ΔcapB- and Lm-vectored vaccines express recombinant B. anthracis, Y. pestis, and F. tularensis immunoprotective antigens in broth and in macrophage-like cells and are non-toxic in mice. Homologous priming-boosting with the LVS ΔcapB-vectored vaccines induces potent antigen-specific humoral and T-cell-mediated immune responses and potent protective immunity against lethal respiratory challenge with all three pathogens. Protection against anthrax was far superior to that obtained with the licensed AVA vaccine and protection against tularemia was comparable to or greater than that obtained with the toxic and unlicensed LVS vaccine. Heterologous priming-boosting with LVS ΔcapB- and Lm-vectored B. anthracis and Y. pestis vaccines also induced potent protective immunity against lethal respiratory challenge with B. anthracis and Y. pestis. The single vaccine platform, especially the LVS ΔcapB-vectored vaccine platform, can be extended readily to other pathogens.Qingmei Jia, Richard Bowen, Barbara Jane Dillon, Saša Masleša-Galić, Brennan T Chang, Austin C Kaidi, Marcus A Horwitz
2047 related Products with: Single vector platform vaccine protects against lethal respiratory challenge with Tier 1 select agents of anthrax, plague, and tularemia.
100ug100ug100ul100ug1 mg100ug1 mg25 mg2.5 mg100ug100ug
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#9376108 //
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[Experimental-practical study of some methods for culturing and counting enterobacteria from raw materials and oral medical preparations].
The technique of enterobacteria enumeration according to F.U., by rivivification in Lactose Broth for 5 h at 37 degrees C and subsequent incubation in Enterobacteriaceae Enrichment Broth, causes artful increases of the number of enterobacteria, often resulting higher than the total microbial charge. To eliminate the drawback we have assayed other two methods: a) incubation in Soybean Casein Digest Broth for 5 h at 37 degrees C, followed by enumeration onto Violet Red Bile Dextrose Agar plates; b) incubation into Soybean Casein Digest Agar plates for 5 h at 37 degrees C, followed by enumeration onto overlayed Violet Red Bile Dextrose Agar. The experimental investigations were performed on 8 artificially stressed (heating, freezing) strain of Enterobacteriaceae and carried out the superiority of the method onto Soybean Casein Digest Agar + Violet Red Bile Dextrose Agar plates. This method was tested on No. 302 samples of raw materials and pharmaceutical preparations, that shown an enterobacterial charge higher than 100 C.F.U. in 24 cases (8%). On the contrary, the enumeration of the enterobacteria according to the F.U. method (Lactose Broth + Enterobacteriaceae Enrichment Broth + Violet Red Bile Dextrose Agar) carried out an enterobacterial charge higher than 100 C.F.U. in 270 cases (89.4%). Indeed, the artful increase of the number of enterobacteria may make unacceptable pharmaceutical preparations suitable for the market. The employment of the method of enumeration of the enterobacteria onto Soybean Casein Digest Agar + Violet Red Bile Dextrose Agar plates is augured.P Casetta, F Negretti, G C Trabattoni