Search results for: Stain Tray Slide Staining System
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Improvement of silver impregnation technique (protargol) to obtain morphological features of protists ciliates, flagellates and opalinates.
The research on ciliates, flagelates and opalinates have been widespread by the utilization of techniques employing silver impregnation (protargol), modified by several authors. However, these are time consuming and its results are variable. The present work is a variant of the technique described by Tuffrau (1964, 1967) showing some adaptations made in our laboratory. The organisms can be preserved by different fixatives (alcoholic Bouin, Stieve's fluid, 2.5% glutaraldehyde and others) and then rinsed in destilled water followed by a fast clarification by 3% sodium hypochloride. If the organism is very sensitive to hypochloride, 4% sodium lauryl sulfate may be used and then washed 3 times in distilled water. The protista can be adhered to the glass slides with Mayer's glycerinated-albumin (1 glycerin vol. to 1 or 2 albumin vol.), diluted in water at a proportion of 1:10 Cv/v., or with 1% polylysine followed by fast washes with distilled water. After the slide preparation, they were covered with a layer of 0,8% Silver proteinate. Right after that, the slide has to be placed in a glass tray lined with moist tissue and covered to prevent the proteinate to dry. The tray was placed in a incubator at 40 degrees - 50 degrees C for 30 minutes. The slides are rinsed for 1 minute. with warm (35 degrees C) distilled water. The development of the material should be done with 0.4% hydroquinone with a maximum incubation time of 1 minute. It should be developed gradually, controlling the silver impregnation intensity by observation under optical microscope. Next, rinse in distilled water for 1 minute, and then, fix in 2,5% Sodium thiosulfate. Rinse the slide for two minutes before dehydrating it in an alcoholic serial 50-100 degrees. Finally rinse the slides in xylene. Mount the slides with Entellan MerckTM or Canada balsam.I D da Silva-Neto
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5 G1 moduleOne 96-Well Strip Micropl100 µg1 KG200 101 module10 mg 30 ml 1 module
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Assessing dentin color changes from nightguard vital bleaching.
At-home bleaching with 10 percent carbamide peroxide in a custom-fitted tray has been reported to change the color of dentin. The purpose of this study was to validate the color change of dentin and to determine whether the color change was uniform or occurred from the outside (the dentinoenamel junction) to the inside (the pulpal wall).A J McCaslin, V B Haywood, B J Potter, G L Dickinson, C M Russell
2762 related Products with: Assessing dentin color changes from nightguard vital bleaching.
50 ug200 assays50 ugN/A 50 ug1 kit50 ug200 assays50 ug100tests
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Keeping plant tissue slices attached to the slide during harsh extraction of polysaccharide.
During extraction of hemicellulose and noncellulose polysaccharides from plant histological slices of plants the morphological integrity of the slices may be disturbed if the reagents extracting hemicellulose and noncellulose polysaccharides are rinsed away by water. Removing the reagents by delicate suction rather than removing the glass tray from the staining dish, followed by desiccation and a rinse in 95% ethanol terminates the treatment without altering the morphology of the tissues.R Jona, L Addari
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100 extractions
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