Search results for: Secretoneurin Rat - Secretogranin II; SgII; Chromogranin C
#22655045 // Save this To Up
Secretogranin II; a protein increased in the myocardium and circulation in heart failure with cardioprotective properties.Several beneficial effects have been demonstrated for secretogranin II (SgII) in non-cardiac tissue. As cardiac production of chromogranin A and B, two related proteins, is increased in heart failure (HF), we hypothesized that SgII could play a role in cardiovascular pathophysiology.
2329 related Products with: Secretogranin II; a protein increased in the myocardium and circulation in heart failure with cardioprotective properties.HIV 1 intergase antigen. anti HSV (II) gB IgG1 (mo Human Macrophage Inflamma Human Macrophage Inflamma EMAP-II Inhibitor Z-ASTD- EMAP-II Inhibitor Z-ASTD- EMAP II Inhibitor Z ASTD EMAP II Inhibitor Z ASTD Mouse Macrophage Inflamma Mouse Macrophage Inflamma Recombinant Human Inhibin Recombinant Human Inhibin
#18221483 // Save this To Up
Immunohistochemical staining of human islet cells with region-specific antibodies against secretogranins II and III.Chromogranins and secretogranins belong to the granin family of proteins, which are expressed in neuroendocrine and nervous tissue. In earlier publications we have described the development of region-specific antibodies against CgA and CgB. In this study we describe antibodies to SgII and SgIII and their usefulness for immunohistochemical staining. Peptides homologous to defined parts of secretogranins II and III were selected and synthesized. Antibodies were raised and immunostainings were performed on normal human pancreas. The SgII 154-165 (N-terminal secretoneurin), SgII 172-186 (C-terminal secretoneurin) and SgIII antibodies immunostained all insulin-immunoreactive cells, most of the glucagon cells and some of the pancreatic polypeptide cells. The SgII 225-242 antibody immunostained only the insulin-containing cells. None of the antibodies immunostained the somatostatin cells. This study is the first observation of the expression of SgIII in human tissues, where we show expression of SgIII in three of the four major islet cell types in human pancreas.
1825 related Products with: Immunohistochemical staining of human islet cells with region-specific antibodies against secretogranins II and III.Mouse Anti-Human Collagen Mouse Anti-Human Collagen Rabbit Anti-Human Collage Rabbit Anti-Human Collage Goat Anti-Human Collagen Rabbit Anti-Human Collage Rabbit Anti-Human Angiote Antibodies, Rabbit: Rabb Antibodies, Rabbit: Rabb Antibodies, Rabbit: Rabb Proteins and Antibodies H Antibodies, Rabbit: Rabb
#18186540 // Save this To Up
Recently identified a novel neuropeptide manserin colocalize with the TUNEL-positive cells in the top villi of the rat duodenum.We recently isolated a novel 40 amino acid neuropeptide designated manserin from the rat brain. Manserin is derived from secretogranin II, a member of granin acidic secretory protein family by proteolytic processing, as previously reported secretoneurin and EM66. Manserin peptide are localized in the endocrine cells of the pituitary. In this study, we further investigated the manserin localization in the digestive system by immunohistochemical analysis using antimanserin antibody. In the duodenum, manserin immunostaining was exclusively observed in the nuclei of top villi instead of cytosol as observed in neurons in our previous study. Interestingly, manserin-positive cells in the duodenum are colocalized with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) positive cells, the cells whose DNA was damaged. Since the top villi of duodenum epithelial cells are known to undergo spontaneous apoptosis during epithelial cell turn over, and since other peptides such as secretoneurin and EM66 derived from SgII have been reported to be cancer-related, these results indicated that manserin peptide may have a role in apoptosis and/or cancer pathogenesis in the digestive organ.
1033 related Products with: Recently identified a novel neuropeptide manserin colocalize with the TUNEL-positive cells in the top villi of the rat duodenum.Thermal Shaker with cooli Rat Anti-CCT theta Antibo FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Normal rat multiple organ Normal rat multiple organ Normal rat multiple organ Multiple organ tumor tiss
#15257142 // Save this To Up
Manserin, a novel peptide from secretogranin II in the neuroendocrine system.We have isolated a novel 40 amino acid neuropeptide, designated manserin, from the rat. Manserin is derived from secretogranin II (SgII), a member of granin acidic secretory protein family by proteolytic processing, as previously reported secretoneurin and EM66. Immunohistochemical analysis using anti-manserin antibody revealed that manserin localized in the endocrine cells of the pituitary anterior lobe, but not in the posterior lobe. Interestingly, manserin never co-localized with ACTH in the anterior pituitary, which is in contrast with SgII, suggesting specific immunoreactivity of the antiserum against manserin. Manserin immunostaining was also observed in the neuronal cells of several hypothalamic nuclei and the neurons in the median eminence. These results suggest that manserin exerts a specific role in the neuroendocrine system.
2253 related Products with: Manserin, a novel peptide from secretogranin II in the neuroendocrine system.anti HSV (II) gB IgG1 (mo EMAP-II Inhibitor Z-ASTD- EMAP-II Inhibitor Z-ASTD- EMAP II Inhibitor Z ASTD EMAP II Inhibitor Z ASTD GLP 1 ELISA Kit, Rat Gluc Glucagon ELISA KIT, Rat G Beta Amyloid (1 40) ELISA Cytoskeleton II Phospho-S Jak Stat II Phospho-Speci NF-kB II Phospho-Specific Thermal Shaker with cooli
#12871990 // Save this To Up
Biochemical characterization and immunocytochemical localization of EM66, a novel peptide derived from secretogranin II, in the rat pituitary and adrenal glands.Characterization of secretogranin II (SgII) mRNA in various vertebrates has revealed selective conservation of the amino acid sequences of two regions of the protein, i.e., the bioactive peptide secretoneurin and a flanking novel peptide that we named EM66. To help elucidate the possible role of EM66, we examined the occurrence as well as the cellular and subcellular distribution of EM66 in rat pituitary and adrenal glands by using a polyclonal antibody raised against the recombinant human EM66 peptide. High-performance liquid chromatography (HPLC) analysis of rat pituitary and adrenal extracts combined with a radioimmunoassay resolved EM66-immunoreactive material exhibiting the same retention time as recombinant EM66. In the rat pituitary, double-labeling immunohistochemical (IHC) studies showed that EM66 immunoreactivity (IR) was present in gonadotrophs, lactotrophs, thyrotrophs, and melanotrophs, whereas corticotrophs were devoid of labeling. EM66-IR was also observed in nerve endings in the neural lobe. Immunocytochemical staining at the electron microscopic level revealed that EM66-IR is sequestered in the secretory granules within gonadotrophs and lactotrophs. In the adrenal medulla, double IHC labeling showed that EM66-IR occurs exclusively in epinephrine-synthesizing cells. At the ultrastructural level, EM66-IR was seen in chromaffin vesicles of adrenomedullary cells. These results demonstrate that post-translational processing of SgII generates a novel peptide that exhibits a cell-specific distribution in the rat pituitary and adrenal glands where it is stored in secretory granules, supporting the notion that EM66 may play a role in the endocrine system.
1566 related Products with: Biochemical characterization and immunocytochemical localization of EM66, a novel peptide derived from secretogranin II, in the rat pituitary and adrenal glands.GLP 1 ELISA Kit, Rat Gluc C Peptide ELISA Kit, Rat Glucagon ELISA KIT, Rat G Anti beta3 AR Human, Poly Rabbit Anti-Rat Androgen CAR,Car,Constitutive andr HIV type O envelope antig Angiotensin II [Lys0](Hum anti HSV (II) gB IgG1 (mo Androgen Receptor (Phosph Androgen Receptor (Phosph EMAP-II Inhibitor Z-ASTD-
#8075827 // Save this To Up
Human and rat primary C-fibre afferents store and release secretoneurin, a novel neuropeptide.Secretoneurin is a recently discovered neuropeptide derived from secretogranin II (SgII). Since this peptide could be detected in the dorsal horn of the spinal cord we studied whether it is localized in and released from primary afferent neurons. Secretoneurin was investigated with immunocytochemistry and radioimmunoassay in spinal cord, dorsal root ganglia and peripheral organs. SgII mRNA was determined in dorsal root ganglia. Normal rats and rats pre-treated neonatally with capsaicin to destroy selectively polymodal nociceptive (C-) fibres were used. Slices of dorsal spinal cord were perfused in vitro for release experiments. Immunocytochemistry showed a distinct distribution of secretoneurin-immunoreactivity (IR) in the spinal cord and, lower brainstem. A particularly high density of fibres was found in lamina I and outer lamina II of the caudal trigeminal nucleus and of the spinal cord. This distribution was qualitatively identical in rat and human post-mortem tissue. Numerous small diameter and some large dorsal root ganglia neurons were found to contain SgII mRNA. Capsaicin treatment led to a marked depletion of secretoneurin-IR in the substantia gelatinosa, but not in other immunopositive areas of the spinal cord and to a substantial loss of small (< 25 microns) SgII-mRNA-containing dorsal root ganglia neurons. Radioimmunoassay revealed a significant decrease of secretoneurin-IR in the dorsal spinal cord, the trachea, heart and urinary bladder of capsaicin-treated rats. Perfusion of spinal cord slices with capsaicin as well as with 60 mM potassium led to a release of secretoneurin-IR. In conclusion, secretoneurin is a neuropeptide which is stored in and released from capsaicin-sensitive, primary afferent (C-fibre) neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
2728 related Products with: Human and rat primary C-fibre afferents store and release secretoneurin, a novel neuropeptide.Primary antibody Histone Rabbit Anti-Human Androge Rabbit Anti-Human Androge Primary antibody NMDANR2 Primary antibody NAC1 An Primary antibody ABCA1 A Rabbit Anti-Human Androge Goat Anti-Human Androgen Rabbit Anti-Rat Androgen CAR,CAR,Constitutive acti CAR,Car,Constitutive andr Recombinant Human Androge
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia