Search results for: Recombinant Human Vacuolar Protein Sorting 24 RECOMBINANT & NATURAL PROTEINS
#32334612 
2020/04/25
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Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei.
Vacuolar H-ATPase (V-ATPase) is a highly conserved protein complex which hydrolyzes ATP and pumps protons to acidify vacuolar vesicles. Beyond its role in pH maintenance, the involvement of V-ATPase in endocytosis is well documented in mammals and plants but is less clear in Trypanosoma brucei.Zhi-Shen Xu, Feng-Jun Li, Geoff Hide, Zhao-Rong Lun, De-Hua Lai
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[The nature of N-terminal signal sequence determines the type of intracellular distribution and effectiveness of export of human growth hormone in Saccharomyces cerevisiae].
Various N-terminal signal peptides (SP) were tested to investigate a human growth hormone (hGH) synthesis, processing and intracellular sorting in yeast. Maximal level of hGH was observed in the case when the mature hGH gene was placed under the control of PHO5 promoter. In this case about 90% of hGH was localized in the cytosol, but some portion was trustworthly detected in microsomes and periplasma in spite of the absence of SP. Addition of own or PHO5 SP resulted in lowering of the synthesis and a difficulty in the prehGH processing. In this case the immunoreactive products were localized mainly in periplasma and vacuoles and to a lesser degree in the cytosol. When hGH gene was placed under the control of the yeast MF alpha 1 promoter and alpha-factor preprosegment was used as SP more then a half (67%) of hGH processed correctly was exported in a medium, the rest was detected in vacuole (17%) and periplasma (8%).A B Tsiomenko, V V Lupashin, S A Morzunov, I V Karpychev, M A El'darov, S N Bezhelarskaia, P M Rubtsov, K G Skriabin, I S Kulaev