Rat monoclonal anti mouse CCL-11 Anti-Mouse antibodies productsSearch results for: Rat monoclonal anti mouse CCL-11 Anti-Mouse antibodies
#36546900 
2022/12/02
To Up
Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry.
The CC chemokine receptor 3 (CCR3) is a receptor for CC chemokines, including CCL5/RANTES, CCL7/MCP-3, and CCL11/eotaxin. CCR3 is expressed on the surface of eosinophils, basophils, a subset of Th2 lymphocytes, mast cells, and airway epithelial cells. CCR3 and its ligands are involved in airway hyperresponsiveness in allergic asthma, ocular allergies, and cancers. Therefore, CCR3 is an attractive target for those therapies. Previously, anti-mouse CCR3 (mCCR3) monoclonal antibodies (mAbs), CMab-3 (rat IgG, kappa), and CMab-4 (rat IgG, kappa) were developed using the Cell-Based Immunization and Screening (CBIS) method. In this study, the binding epitope of these mAbs was investigated using flow cytometry. A CCR3 extracellular domain-substituted mutant analysis showed that CMab-3, CMab-4, and a commercially available mAb (J073E5) recognized the N-terminal region (amino acids 1-38) of mCCR3. Next, alanine scanning was conducted in the N-terminal region. The results revealed that the Ala2, Phe3, Asn4, and Thr5 of mCCR3 are involved in CMab-3 binding, whereas Ala2, Phe3, and Thr5 are essential to CMab-4 binding, and Ala2 and Phe3 are crucial to J073E5 binding. These results reveal the involvement of the N-terminus of mCCR3 in the recognition of CMab-3, CMab-4, and J073E5.Nami Tateyama, Teizo Asano, Hiroyuki Suzuki, Guanjie Li, Takeo Yoshikawa, Tomohiro Tanaka, Mika K Kaneko, Yukinari Kato
