Search results for: Rat Embryo PrimaCell™2: Normal Fetal Dermal Fibroblasts, primary cell culture
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Primary study on transplantation of endothelialized dermal equivalents into normal rats.
This study was designed to determine the ability of human umbilical vein endothelial cells (HUVEC) in dermal equivalent (DE) to form microvessel-like tubes after transplantation into normal rats. A mixture of rat fibroblasts and HUVEC was inosculated into collagen-chitosan sponges to prepare endothelialized dermal equivalents (EDE). After culture in vitro for 24 hours, inosculated cells dispersed throughout the sponges and the equivalents were transplanted subcutaneously into the back of normal Lewis rats. Anti-human specific CD31 antibody was used for immunohistochemical localization of human endothelial cells in sections of EDE excised from rats after grafting. HUVEC in EDE organized into microvessel-like tubes at the end of the first week after transplantation, which still persisted after two weeks. The host microvessels began to pervade both DE and EDE during the second week after transplantation. These results demonstrated that HUVEC in EDE was able to persist and form microvessel-like tubes after transplantation into normal rats, and this is the first time to transplant DE containing HUVEC into normal rats.Juan Zhou, Lingrong Liu, Xuemin Li, Han Chen, Qiqing Zhang
2084 related Products with: Primary study on transplantation of endothelialized dermal equivalents into normal rats.
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Cellular interactions promote tissue-specific function, biomatrix deposition and junctional communication of primary cultured hepatocytes.
Hepatocytes, prepared from normal adult rat liver, were seeded onto a collagen substratum and cultured alone or in the presence of rat liver endothelial cells. When hepatocytes were cultured alone in a hormonally defined serum-free medium, decreased albumin production and rapid morphological deterioration of bile canaliculi structures and gap junctions occurred within 4 to 5 days. In contrast, hepatocytes cocultured with liver mesenchymal cells remained morphologically intact and biochemically functional for at least 4 weeks. They reorganized into small islands, continued to secrete high levels of albumin, did not express alpha-fetoprotein (a fetal marker), and remained strongly dye coupled. All of the hepatocytes synthesized albumin and retained their gap junctional channels. No junctional communication was observed between hepatocytes and endothelial cells. Long fibers containing fibronectin, Type I collagen and laminin distributed over the hepatocytes were induced in coculture but never appeared in hepatocytes cultured alone. Moreover, supplementation of the hormonally defined medium with phenobarbital and dimethyl sulfoxide, both of which improve the life span and functional activities of cultured hepatocytes, failed to induce reticulin fiber formation in pure culture of hepatocytes. The modulation of albumin secretion, biomatrix deposition and junctional communication observed in hepatocytes cultured with sinusoidal liver cells was also obtained when hepatocytes were in association with various epithelial or mesenchymal cells [rat liver epithelial cells (T51B), mouse embryonic fibroblasts (NIH 3T3), human or rat dermal fibroblasts and bovine aorta endothelial cells (AG 4762)].F Goulet, C Normand, O Morin