Search results for: Rat CK_M ELISA
#20378983 2010/04/07 To Up
Proteomic identification of serum proteins associated with stress-induced gastric ulcers in fasted rats.
Several physical and psychological stresses frequently become triggers for gastrointestinal disorders such as ulcer. In this study, we tried to identify serum proteins as potential biomarkers for the evaluation of stress-induced gastric ulcer. By proteomic analysis using rats with gastric ulcer induced by water immersion and restraint (WIR) stress as an animal model, we found quantitative changes in several serum proteins, including creatine kinase muscle M chain (CK-M) and apolipoprotein A-IV (ApoA4) in the stressed rats. On western blotting and enzyme-linked immunosorbent assay (ELISA), we confirmed that serum CK-M was remarkably increased by WIR stress. However, ApoA4 appeared to be decreased by fasting, but not WIR stress, which is usually applied prior to WIR stress. The findings suggest that these two serum proteins might be useful as biomarkers, CK-M for stress-induced gastric ulcer and ApoA4 for starvation.Ayako Kato, Hirotaka Naitou, Makoto Namioka, Masanobu Akimoto, Takeshi Ishii, Hiroyuki Sakakibara, Kayoko Shimoi, Tsutomu Nakayama, Norio Ohashi
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96T25ml1010010100ug50 50200 96 assaysRelated Pathways
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