Search results for: Rabbit Polyclonal Antibody; Leptin , Anti-Human
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[A highly sensitive enzyme-linked immunosorbent assay for measurement of leptin secretion in human adipocytes].
To establish a highly sensitive and specific ELISA method for measurement of leptin and further to study the secretion of leptin during human preadipocytes differentiation and effects of troglitazone.Ming Li, Jin-hua Yin, Kui Zhang, Cong-yuan Wu
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1 kit4 Membranes/Box4 Arrays/Slide16 Arrays/Slide4 Membranes/Box4 Arrays/Slide4 Membranes/Box4 Arrays/Slide4 Membranes/Box4 Arrays/Slide4 Membranes/Box96 tests
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#17234799 2007/01/18
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Leptin receptors in human skeletal muscle.
Human skeletal muscle expresses leptin receptor mRNA; however, it remains unknown whether leptin receptors (OB-R) are also expressed at the protein level. Fourteen healthy men (age = 33.1 +/- 2.0 yr, height = 175.9 +/- 1.7 cm, body mass = 81.2 +/- 3.8 kg, body fat = 22.5 +/- 1.9%; means +/- SE) participated in this investigation. The expression of OB-R protein was determined in skeletal muscle, subcutaneous adipose tissue, and hypothalamus using a polyclonal rabbit anti-human leptin receptor. Three bands with a molecular mass close to 170, 128, and 98 kDa were identified by Western blot with the anti-OB-R antibody. All three bands were identified in skeletal muscle: the 98-kDa and 170-kDa bands were detected in hypothalamus, and the 98-kDa and 128-kDa bands were detected in thigh subcutaneous adipose tissue. The 128-kDa isoform was not detected in four subjects, whereas in the rest its occurrence was fully explained by the presence of intermuscular adipose tissue, as demonstrated using an anti-perilipin A antibody. No relationship was observed between the basal concentration of leptin in serum and the 170-kDa band density. In conclusion, a long isoform of the leptin receptor with a molecular mass close to 170 kDa is expressed at the protein level in human skeletal muscle. The amount of 170-kDa protein appears to be independent of the basal concentration of leptin in serum.Borja Guerra, Alfredo Santana, Teresa Fuentes, Safira Delgado-Guerra, Alfredo Cabrera-Socorro, Cecilia Dorado, Jose A L Calbet