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Search results for: Human pre_microRNA Expression Construct Lenti_miR_181a_2

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#29297389   2017/09/20 To Up

A novel method to identify pre-microRNA in various species knowledge base on various species.

More than 1/3 of human genes are regulated by microRNAs. The identification of microRNA (miRNA) is the precondition of discovering the regulatory mechanism of miRNA and developing the cure for genetic diseases. The traditional identification method is biological experiment, but it has the defects of long period, high cost, and missing the miRNAs that but also many other algorithms only exist in a specific period or low expression level. Therefore, to overcome these defects, machine learning method is applied to identify miRNAs.
Tianyi Zhao, Ningyi Zhang, Ying Zhang, Jun Ren, Peigang Xu, Zhiyan Liu, Liang Cheng, Yang Hu

1140 related Products with: A novel method to identify pre-microRNA in various species knowledge base on various species.

100ul 100ul 100ul 100ul100 ug 50 ug 100ug 100ul50ul 100ul 100ul 100ul

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#28575281   // To Up

Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7.

We evaluate a knockdown-replacement strategy mediated by mirtrons as an alternative to allele-specific silencing using spinocerebellar ataxia 7 (SCA7) as a model. Mirtrons are introns that form pre-microRNA hairpins after splicing, producing RNAi effectors not processed by Drosha. Mirtron mimics may therefore avoid saturation of the canonical processing pathway. This method combines gene silencing mediated by an artificial mirtron with delivery of a functional copy of the gene such that both elements of the therapy are always expressed concurrently, minimizing the potential for undesirable effects and preserving wild-type function. This mutation- and single nucleotide polymorphism-independent method could be crucial in dominant diseases that feature both gain- and loss-of-function pathologies or have a heterogeneous genetic background. Here we develop mirtrons against ataxin 7 with silencing efficacy comparable to shRNAs, and introduce silent mutations into an ataxin 7 transgene such that it is resistant to their effect. We successfully express the transgene and one mirtron together from a single construct. Hence, we show that this method can be used to silence the endogenous allele of ataxin 7 and replace it with an exogenous copy of the gene, highlighting the efficacy and transferability across patient genotypes of this approach.
Helen J Curtis, Yiqi Seow, Matthew J A Wood, Miguel A Varela

2036 related Products with: Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7.

100 μg1 mg100 μg5mg100 μg25 mg100ug Lyophilized100ug1-99 mg/ml/ea price x 2

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#25161167   2014/08/26 To Up

Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells.

Noncoding microRNAs (miRNAs or miRs) have been revealed as critical epigenetic factors in the regulation of various cellular processes, including drug metabolism and disposition. However, research on miRNA functions is limited to the use of synthetic RNA and recombinant DNA agents. Herein, we show that novel pre-miRNA-27b (miR-27b) agents can be biosynthesized in Escherichia coli using recombinant RNA technology, and recombinant transfer RNA (tRNA)/mir-27b chimera was readily purified to a high degree of homogeneity (>95%) using anion-exchange fast protein liquid chromatography. The tRNA-fusion miR-27b was revealed to be processed to mature miRNA miR-27b in human carcinoma LS-180 cells in a dose- and time-dependent manner. Moreover, recombinant tRNA/miR-27b agents were biologically active in reducing the mRNA and protein expression levels of cytochrome P450 3A4 (CYP3A4), which consequently led to lower midazolam 1'-hydroxylase activity. These findings demonstrate that pre-miRNA agents can be produced by recombinant RNA technology for functional studies.
Mei-Mei Li, Wei-Peng Wang, Wen-Juan Wu, Min Huang, Ai-Ming Yu

1443 related Products with: Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells.

50 1 mg100 10 10 ug25 1 mg100 µg200ul10

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#21436129   2011/03/23 To Up

Prediction of novel pre-microRNAs with high accuracy through boosting and SVM.

High-throughput deep-sequencing technology has generated an unprecedented number of expressed short sequence reads, presenting not only an opportunity but also a challenge for prediction of novel microRNAs. To verify the existence of candidate microRNAs, we have to show that these short sequences can be processed from candidate pre-microRNAs. However, it is laborious and time consuming to verify these using existing experimental techniques. Therefore, here, we describe a new method, miRD, which is constructed using two feature selection strategies based on support vector machines (SVMs) and boosting method. It is a high-efficiency tool for novel pre-microRNA prediction with accuracy up to 94.0% among different species.
Yuanwei Zhang, Yifan Yang, Huan Zhang, Xiaohua Jiang, Bo Xu, Yu Xue, Yunxia Cao, Qian Zhai, Yong Zhai, Mingqing Xu, Howard J Cooke, Qinghua Shi

1751 related Products with: Prediction of novel pre-microRNAs with high accuracy through boosting and SVM.

Up to 200 ml cultures10x96, 2.0ml cultures10, 10ml whole blood 4 Membranes/Box4 Arrays/Slide2 Sample Kit100 mg 6 ml Ready-to-use

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