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Generation and characterization of pan-specific anti-acetyllysine antibody.

Pan-specific anti-acetylated lysine antibody is a valuable tool to detect, validate, and quantify protein lysine acetylation. Compared to site-specific acetyllysine antibodies that are not readily available in most cases, polyclonal pan-specific acetyllysine antibodies can be generated with synthetic antigen of a carrier protein that has its lysine residues chemically acetylated. Here, we describe protocols of synthesizing acetylated ovalbumin (OVA), immunizing rabbits, and characterizing pan-specific polyclonal anti-acetylated lysine antibodies.

1977 related Products with: Generation and characterization of pan-specific anti-acetyllysine antibody.

MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD RABBIT ANTI GSK3 BETA (pS Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia

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Evaluation of an enzyme-linked immunosorbent assay based on crude Leishmania histone proteins for serodiagnosis of human infantile visceral leishmaniasis.

Human visceral leishmaniasis (VL) is routinely diagnosed by detecting IgG that specifically binds to Leishmania antigens. The enzyme-linked immunosorbent assay (ELISA) remains a widely used method. However, the biggest challenge remains the choice of antigen with the highest specificity and sensitivity. This study is aimed at assessing the diagnostic performances of crude Leishmania histone (CLH) protein-based ELISAs in Mediterranean VL patients. The CLH proteins were biochemically purified from promastigote nuclear extracts. Their reactivities were analyzed by Western blotting (WB) using rabbit polyclonal antibodies against Leishmania recombinant histones and sera from VL patients, respectively. Then, the diagnostic potential of CLH proteins was validated by the CLH-based ELISA using 42 infantile VL patients' sera and 70 control subjects. The CLH-based ELISA performance was compared to that of the soluble Leishmania antigen (SLA)- and the recombinant K39 (rK39)-based ELISAs. Analysis of the WB profile with the use of polyclonal antibodies confirmed the histone origin of low molecular mass proteins (12 to 16 kDa). All VL samples tested presented antibodies reacting against different antigen fractions; however, recognition patterns were different depending on the reactivity of each serum. CLH-based ELISA showed an excellent ability to discriminate between VL cases and healthy controls (97.6% sensitivity and 100% specificity). It had a diagnostic performance similar to that of rK39-based ELISA (97.6% sensitivity and 97.1% specificity, P = 0.5) and a better serodiagnosis accuracy than the SLA-based ELISA (85.7% sensitivity and 90% specificity, P < 0.05). Therefore, crude Leishmania histone extract could be a valuable antigen for clinical use.

1411 related Products with: Evaluation of an enzyme-linked immunosorbent assay based on crude Leishmania histone proteins for serodiagnosis of human infantile visceral leishmaniasis.

Mouse anti-human type I c Rat anti-human type I col Rat anti-human type I col Human monkey anti-chick t Human monkey anti-chick t Human monkey anti-bovine Human monkey anti-bovine Human monkey anti-porcine Human monkey anti-porcine Human monkey anti-human t Human monkey anti-human t Human monkey anti-chick t

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K16-biotinylated histone H4 is overrepresented in repeat regions and participates in the repression of transcriptionally competent genes in human Jurkat lymphoid cells.

Holocarboxylase synthetase (HCS) catalyzes the binding of biotin to lysine (K) residues in histones H3 and H4. Histone biotinylation marks are enriched in repressed loci, including retrotransposons. Preliminary studies suggested that K16 in histone H4 is a target for biotinylation by HCS. Here we tested the hypotheses that H4K16bio is a real histone mark in human chromatin and that H4K16bio is overrepresented in repressed gene loci and repeat regions. Polyclonal rabbit anti-human H4K16bio was generated and affinity purified. An extensive series of testing with synthetic and natural targets confirmed that this new antibody is specific for H4K16bio. Using anti-H4K16bio and chromatin immunoprecipitation assays, we demonstrated that H4K16bio is overrepresented in repeat regions [pericentromeric alpha satellite repeats and long terminal repeats (LTR)] compared with euchromatin promoters. H4K16bio was also enriched in the repressed interleukin-2 gene promoter in human lymphoid cells; transcriptional activation of the interleukin-2 gene by mitogens and phorbol esters coincided with a depletion of the H4K16bio mark at the gene promoter. The enrichment of H4K16bio depended on biotin supply; the enrichment at LTR22 and promoter 1 of the sodium-dependent multivitamin transporter (SMVT) was greater in biotin-supplemented cells compared with biotin-normal and biotin-deficient cells. The enrichment of H4K16bio at LTR15 and SMVT promoter 1 was significantly lower in fibroblasts from an HCS-deficient patient compared with an HCS wild-type control. We conclude that H4K16bio is a real phenomenon and that this mark, like other biotinylation marks, is overrepresented in repressed loci where it marks HCS docking sites.

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Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Goat Anti-Human Laforin ( Mouse Anti-Human Interleu Mouse Anti-Human Interleu Macrophage Colony Stimula Macrophage Colony Stimula interleukin 17 receptor C Rabbit Anti-intestinal FA Rabbit Anti-APIP Apaf1 In Rabbit Anti-APIP Apaf1 In Rabbit Anti-TNIP2 ABIN2 T

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Characterization and quality control of antibodies used in ChIP assays.

We present here the very robust characterization and quality control (QC) process that we have established for our polyclonal antibodies, which are mainly directed against targets relevant to the epigenetics field such as modified histones, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation and relies on the use of antibodies to select the protein of interest and then precipitate and identify the DNA associated to it. We have optimized in-house all protocols and reagents needed from the first to the last step of antibody characterization. First, following immunizations, the rabbit crude serum is tested for immune response. Whether or not the antibody is specific is determined in further characterizations. Then, only specific antibodies are tested in ChIP using an optimized method which is ideal for antibody screening. Once QC is established for one antibody, it is used to similarly characterize each antibody batch in order to supply researchers in a reproducible manner with validated antibodies. All in all, this demonstrates that we develop epigenetics research tools based on everyday's researcher's needs by providing batch-specific fully characterized ChIP-grade antibodies.

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Cell Cycle Control Phosph HIV1 integrase antibody, Goat Anti- TRPM8, (intern Goat Anti- TFAP2D, (inter Goat Anti- T1R3, (interna Goat Anti-Human Synaptota Goat Anti-Human STK39 SPA Goat Anti-Human SPHK1, (i Goat Anti-Human SODD, (in Goat Anti-Human SIGLEC8, Goat Anti-Human SH2D4A, ( Goat Anti-Human SEPT7, (i

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Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization.

To investigate the expression of oocyte-specific linker histone protein (hH1FOO) in human ovaries and its incorporation into sperm chromatin after intracytoplasmic sperm injection (ICSI).

1635 related Products with: Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization.

B-cell linker protein ant NATIVE HUMAN PROLACTIN, P Mouse Anti-Human Neuron S Recombinant Human Androge Prostate-Specific Antigen NATIVE HUMAN PROLACTIN, P Recombinant Mn SOD (Human Anti C Reactive Protein A Bone Morphogenetic Protei Bone Morphogenetic Protei Bone Morphogenetic Protei Bone Morphogenetic Protei

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Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels.

In addition to DNA sequence information, site-specific histone modifications are another important determinant of gene expression in a eukaryotic organism. We selected four modification sites in common histones that are known to significantly impact chromatin function and generated monoclonal or polyclonal antibodies that recognize each of those site-specific modifications. We used these antibodies to demonstrate that the site-specific histone modification levels remain relatively constant in different organs of the same organism. We also compared the levels of selected histone modifications among several representative organisms and found that site-specific modifications are highly variable among different organisms, providing new insight into the evolutionary divergence of specific histone modifications.

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RABBIT ANTI GSK3 BETA (pS MOUSE ANTI BOVINE ROTAVIR Androgen Receptor (Phosph Androgen Receptor (Phosph Androgen Receptor (Ab 650 Anti dimethyl Histone H3 Rabbit Anti-intestinal FA Rabbit Anti-IAA (Indole-3 Rabbit Anti-IAA (Indole-3 Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Integrin alph Rabbit Anti-APIP Apaf1 In

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The transcriptional co-repressor C-terminal binding protein (CtBP) associates with centrosomes during mitosis.

CtBP is a corepressor of transcription that acts by inhibiting coactivators and recruiting to promoter control elements (via interactions with DNA-binding transcription factors) a complex of proteins that modify histones, repress transcription and silence gene expression. There are two highly homologous genes, CtBP1 and CtBP2 that encode CtBP. In addition, a CtBP1-related protein has been described that has 11 fewer amino acids at its N-terminus than CtBP1. This variant of CtBP1--known as CtBP3 or BARS [Brefeldin A (BFA) ribosylated substrate]--plays a critical role in the fragmentation of the Golgi complex at the onset of mitosis. Although there are some reports of CtBP with a cytoplasmic distribution after transfection, it is unclear how in normal cells a nuclear corepressor regulates the behavior of the Golgi complex at the onset of mitosis. Using polyclonal rabbit antibodies that were raised against a human CtBP1-GST fusion-protein, here we show by immunofluorescence laser-scanning confocal microscopy that in mitotic cells a species of CtBP becomes associated with the centrosomes at the onset of prophase and then throughout mitosis. The association can be seen in both cycling mitotic cells and nocodazole-arrested cells. The interaction was confirmed by coimmunoprecipitation and centrosome isolation. Since centrosomes are considered to be the organising centre for Golgi morphogenesis, the interaction demonstrated here may explain how a nuclear corepressor of transcription can exert a regulatory effect on the Golgi complex at a specific stage of the cell cycle.

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Acyl CoA binding Protein E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran Taq SSB (Single Stranded Taq SSB (Single Stranded Mouse Anti-Human Retinol S100 alpha - Rabbit polyc Human S100 Calcium Bindin ribosome binding protein ribosome binding protein

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Characterization of immunosuppressive factors expressed in serum by rat tolerogenic liver transplantation.

In a rat tolerogenic model of orthotopic liver transplantation (OLT), recipient serum after OLT (post-OLT serum) possesses strong immunosuppressive activity. This study aimed to identify immunosuppressive factors present in early post-OLT serum.

2346 related Products with: Characterization of immunosuppressive factors expressed in serum by rat tolerogenic liver transplantation.

Sterile filtered rat ser Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Insulin 1 (Rat), syntheti Goat Anti-Rat MARCH10, (i Goat Anti-Mouse, Rat DLL1 Goat Anti-Human, Mouse, R Goat Anti-Human, Mouse, R Goat Anti-Rat Connexin 43 Goat Anti-Human, Rat CHRN Rat Anti-Mouse Interleuki

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Development of a PAN-specific, affinity-purified anti-acetylated lysine antibody for detection, identification, isolation, and intracellular localization of acetylated protein.

Acetylation on the lysine residue is an important event of posttranslational modification of proteins. In this study, we developed a simple method to produce and to affinity purify the specific anti-acetylated lysine polyclonal antibody, which is useful for the detection, identification, isolation, and intracellular localization of acetylated proteins on the lysine residues. We utilized the chemically acetylated hemocyanin of keyhole limpets (KLH) as an immunogen to raise the immune serum and to isolate the population of the acetylated lysine specific antibody using the immobilized acetylated lysine as immunoaffinity-ligand. The isolated antibody was tested to be useful for ELISA, immunoblotting detection, immunofluorescent localization, and affinity isolation of the acetylated proteins.

1963 related Products with: Development of a PAN-specific, affinity-purified anti-acetylated lysine antibody for detection, identification, isolation, and intracellular localization of acetylated protein.

Anti-Acetylated Lysine (A Anti-Acetylated Lysine (A Anti-Acetylated Lysine (A Anti-Acetylated Lysine (A MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD Rabbit anti KLH Antibody Rabbit anti SRC1 Antibody Rabbit anti DDB1 Antibody RABBIT ANTI GSK3 BETA (pS MOUSE ANTI CANINE DISTEMP MOUSE ANTI HUMAN CD15, Pr

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Liver transplantation-induced antihistone H1 autoantibodies suppress mixed lymphocyte reaction.

In a rat model of orthotopic liver transplantation (OLT), recipient serum after OLT (post-OLT serum) has been reported to prevent allograft rejection. However, the molecular identities of immunosuppressive factors, which are in the early stage of post-OLT, remain elusive. This study was aimed to identify immunodominant suppressive factors present in early post-OLT serum.

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Carnitine O palmitoyltran Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon 5(6) FAM [5 (and 6) Carbo Human Epstein-Barr Virus 2X Reaction Buffer 2X Reaction Buffer 20 ml Reaction Buffer, 2X 2X Reaction Buffer 2X Reaction Buffer 80 ml

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