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Search results for: Fibronectin, Clone 2B6_F9, Mab anti_Human

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#24502396   2014/02/06 To Up

Establishment of monoclonal anti-human CD26 antibodies suitable for immunostaining of formalin-fixed tissue.

A T cell costimulatory molecule with dipeptidyl peptidase IV (DPPIV) activity in its extracellular region, CD26 is a multifunctional molecule associated with various proteins such as adenosine deaminase, caveolin-1, CXCR4, collagen, and fibronectin, while playing an important role in the regulation of inflammatory responses and tumor biology. We have focused on CD26 as a novel therapeutic target for various tumors and immune disorders, and have developed a humanized anti-CD26 monoclonal antibody (mAb), YS110, which is currently being evaluated in a phase I clinical trial for patients with CD26-expressing tumors, including malignant mesothelioma. Since detection of tumor CD26 expression is required for determining potential eligibility for YS110 therapy, the development of anti-human CD26 mAb that can clearly and reliably detect the denatured CD26 molecule in the formalin-fixed paraffin-embedded tissues is critical.
Ryo Hatano, Taketo Yamada, Shuji Matsuoka, Satoshi Iwata, Hiroto Yamazaki, Eriko Komiya, Toshihiro Okamoto, Nam H Dang, Kei Ohnuma, Chikao Morimoto

1683 related Products with: Establishment of monoclonal anti-human CD26 antibodies suitable for immunostaining of formalin-fixed tissue.

0.1ml (1mg/ml)100 μg100ul0.1 mg50ul50 100 TESTS0.1 mg100.00 ug50 25 µg

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#3261752   // To Up

Production of a fibronectin-associated lymphokine by cloned mouse T cells.

Azobenzenearsonate-specific cloned mouse T cells able to transfer delayed hypersensitivity reactions in vivo produced macrophage agglutination factor (MaggF) after stimulation with mitogen or antigen in vitro. Mitogen (Con A) elicited MAggF production directly from T cells. Responses to Ag were Ag-specific, required syngeneic accessory cells in addition to T cells, and were independent of T cell fine specificity for azobenzenearsonate. Mouse MAggF shared a number of biochemical and immunochemical properties with the fibronectins (FN): 1) high Mr similar to that of plasma FN; 2) binding to gelatin, heparin, and polyclonal antibodies and mAb specific for cellular and plasma FN; 3) inhibition of activity in solution by monoclonal anti-human FN directed against plasma FN gelatin-binding domain; and 4) action on peritoneal exudate macrophages mediated through a FN-receptor cross reactive with one on human monocytes. MAggF production required active protein synthesis and was associated with significant increases in gelatin-binding immunoreactive FN (Mr 440 kDa on immunoblotting) in culture supernatants and T cell lysates. Metabolically labeled peptides could be precipitated by anti-FN from culture supernatants of activated T cells. Stimulated cultures contained significantly more cells with immunohistologically demonstrable cytoplasmic FN than unstimulated control cultures. We suggest that T cell FN is a distinct species of cellular FN which may play an important role in mediating delayed hypersensitivity inflammatory reactions in vivo.
H P Godfrey, L S Canfield, H L Kindler, C V Angadi, J J Tomasek, J W Goodman

1781 related Products with: Production of a fibronectin-associated lymphokine by cloned mouse T cells.

100.00 ug100.00 ug0.1ml (1mg/ml)0.1ml (1mg/ml)25 TESTS100 μg1 mg1 mg0.1 mg0.1ml96 Tests/kit

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