Search results for: Exonuclease III
#29529509 // Save this To Up
Ce(III, IV)-MOF electrocatalyst as signal-amplifying tag for sensitive electrochemical aptasensing.Metal-organic frameworks (MOFs) as a new class of porous materials have attracted increasing attention in the field of biomimetic catalysis. This study firstly reports a mixed valence state Ce-MOF possessing intrinsic catalytic activity towards thionine (Thi), and its application in constructing an amplified electrochemical aptasensor for thrombin detection. As noticed, the novel catalytic process combines the advantages of 3D infinite extension of the Ce(III, IV)-MOF skeleton containing large amounts of catalytic sites and spontaneous recycling of the Ce(III)/Ce(IV) for electrochemical reduction of Thi, thereby presenting amplified electrochemical signals. To further improve the aptasensor performance, the high selectivity of proximity binding-induced DNA strand displacement and high efficiency of exonuclease III-assisted recycling amplification were incorporated into the assay. The aptasensor was employed to detect thrombin in complex serum samples, which shows high sensitivity, specificity, stability and reproducibility. This work offers an opportunity to develop MOF-based electrocatalyst as signal-amplifying tag for versatile bioassays and catalytic applications.
1170 related Products with: Ce(III, IV)-MOF electrocatalyst as signal-amplifying tag for sensitive electrochemical aptasensing.Glucose Assay With the La Cultrex 24 Well Collagen Cultrex 96 Well Collagen Cultrex In Vitro Angiogen Cal-520™ PBX Calcium As Cal-520™ PBX Calcium As Endothelial Tube Formatio QuantiChrom™ Formaldehy QuantiChrom™ Formaldehy EnzyChrom™ NAD NADH Ass Human Antithrombin III to Formate Assay Kit
#29526891 // Save this To Up
A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification.In this paper, we describe a simple and highly sensitive fluorescence strategy of mercury ions based on exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. With an ultra high sensitivity (1 pM), our strategy has been simple and cost-effective, which does not need any artificial modification fluorescence groups, and can be carried out in a pot. It also shows excellent selectivity. Therefore, our new method provides an effective platform for mercury-ion detection.
2362 related Products with: A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification.Beta Amyloid (1 42) High MarkerGeneTM Fluorescent Amplite™ Fluorimetric F Ionomycin (free acid) Cell Meter™ Apoptotic a EnzyChrom™ NAD NADH Ass EnzyChrom™ Free Fatty A MarkerGeneTM in vivo lacZ 8 Octadecyloxypyrene 1,3, 4 Methylumbelliferyl sulf Ionomycin free acid CAS N Fatty acid free heat sho
#29522905 // Save this To Up
Stochastic DNA walker for electrochemical biosensing sensitized with gold nanocages@graphene nanoribbons.A target-driven stochastic DNA walking electrochemical biosensor sensitized with gold nanocages@graphene nanoribbons (Au NCs@GNRs) was explored for sensitive detection of target DNA. Benefited from the large surface area and excellent conductivity of Au NCs and GNRs, the proposed sensing platform not only improved the electron transfer kinetics involved in electrochemical reactions, but also enhanced the loading capability for stem-loop structural DNA segment (H). Upon the addition of target DNA, the hairpin structure of H was opened and H:target DNA duplex was formed based on toehold-mediated DNA strand displacement. In the presence of exonuclease III (Exo III), the H:target DNA duplex was digested. As a result, target DNA spontaneously dissociated from H:target DNA duplex and then hybridized with another H strand. Therefore, the continuous locomotion of target DNA unceasingly triggered new digestion process from near to far along the electrode surface, resulting in great signal amplification. The proposed strategy exhibited excellent detection performances for DNA analysis in complex matrix such as human serum, which illuminated the practical application field of the sensing platform.
1028 related Products with: Stochastic DNA walker for electrochemical biosensing sensitized with gold nanocages@graphene nanoribbons.Rabbit Anti-DNase gamma P Rabbit Anti-DNA Polymeras Cellufine Formyl , 50 ml Cellufine Formyl Media Cellufine Formyl , 500 ml Cellufine Formyl Media Cellufine Formyl Media Protease, DNASE free hea Protease, DNASE free hea Protease, DNASE free hea Formalin Solution (20%) Formalin Solution (20%)
#29397702 // Save this To Up
Plasmonic Enhancement Coupling with Defect-Engineered TiO: A Mode for Sensitive Photoelectrochemical Biosensing.This work demonstrates that the photoelectric response of defect-engineered TiOmodified with Au nanoparticles can be modulated by oxygen vacancy concentration and excitation wavelength. When strongly plasmonic Au nanoparticles are anchored to defect-engineered TiOby DNA hybridization, several times plasmonic enhancement of photocurrent occurs under 585 nm excitation, and it is employed as a novel signaling mode for developing an improved photoelectrochemical sensing platform. This signaling mode combined with exonuclease III-assisted target recycling amplification exhibits excellent analytical performance, which provides a novel photoelectrochemical detection protocol.
1912 related Products with: Plasmonic Enhancement Coupling with Defect-Engineered TiO: A Mode for Sensitive Photoelectrochemical Biosensing.MOUSE ANTI BOVINE ROTAVIR Bone Morphogenetic Protei Growth Differentiation Fa Amplite™ Fluorimetric F MOUSE ANTI BORRELIA BURGD Alpha-soluble NSF attachm succinate-CoA ligase, GDP formin-like 1 antibody So succinate-CoA ligase, ADP Primary antibody Caspase Primary antibody FLIP An Beta Amyloid (1 42) High
#29377586 // Save this To Up
A Universal Upconversion Sensing Platform for the Sensitive Detection of Tumour-Related ncRNA through an Exo III-Assisted Cycling Amplification Strategy.Here, a sensitive and universal noncoding RNA (ncRNA) upconversion sensing nanoplatform is developed. Gold nanoparticles bearing one hairpin DNA (Hp) molecule are conjugated to the linker DNA modified NaYF:Yb, Er@NaYFupconversion nanoparticles by DNA hybridization, leading to quenching of the upconversion emission through fluorescence resonance energy transfer. A signal DNA (SDNA) sequence is designed to open Hp, recovering the upconversion emission. To achieve universality and high sensitivity of the nanoprobe, an exonuclease III (Exo III)-assisted cycling amplification strategy is introduced. A multifunctional hairpin DNA (mHp) containing ncRNA recognition sequence and SDNA sequence is designed to recognize ncRNA and trigger Exo III as a biocatalyst to stepwise disintegrate itself, releasing both ncRNA and SDNA. The released ncRNA can be reused to release more SDNA, which greatly improves the sensing sensitivity. By changing the recognition portion of mHp, various ncRNA can be detected. The sensitive detection of both homeobox (HOX) transcript antisense RNA segment and miR-21 is achieved with this novel strategy, even in human serum, indicating the universality and sensitivity of the proposed strategy. Additionally, the expression level of miR-21 in human breast cancer cell (MCF-7) lysate is successfully measured, suggesting its potential in clinical diagnosis.
1217 related Products with: A Universal Upconversion Sensing Platform for the Sensitive Detection of Tumour-Related ncRNA through an Exo III-Assisted Cycling Amplification Strategy.EXOC7 antibody Host Goat Factor VIII Related Anti Factor VIII Related Anti Factor VIII Related Anti MOUSE ANTI BOVINE ROTAVIR Detection Buffer A&B Anti Detection Buffer C&D Anti Rabbit Anti-Human EXOC2 MOUSE ANTI BORRELIA BURGD Fos-related antigen 2 - N Tubulin beta-III Antibody EXOC7 antibody Source Rab
#29331428 // Save this To Up
Heating enhanced sensitive and selective electrochemical detection of Hgbased on T-Hg-T structure and exonuclease III-assisted target recycling amplification strategy at heated gold disk electrode.A sensitive and selective electrochemical Hgsensor was developed based on T-Hg-T structure and exonuclease (Exo) III -assisted target recycling amplification at heated gold disk electrode (HAuDE). First, a DNA signal probe P1 was for the first time designed and labeled with ferrocene (Fc) near the attached SH-5'-end, so as to shorten the distance between Fc and the electrode and enhance the initial current of Fc compared with that labeled at the 3'-end far from the electrode. Then the signal amplification was achieved by Exo III-assisted Hgrecycling. Briefly, the P1 was complementary to the assistant DNA P2 except the T-T mismatches. In the presence of Hg, the P1 self-assembled on the HAuDE could hybridize with P2 and form DNA duplex with blunt end at the 3'- terminus, triggering Exo III to stepwise digest mononucleotides from the 3'-terminus of P1, ultimately liberating Hgand P2, which could be "recycled", resulting in the digestion of a large amount of P1 and significantly decrease the amount of Fc. The electrochemical signal difference before and after digestion was proportional to the Hgconcentration. Furthermore, during the digestion period, the Exo III activity could be significantly increased by elevating the electrode temperature, great improving the sensitivity and efficiency for Hgdetection. A detection limit of 6.2 pM (S/N = 3) could be obtained with an electrode temperature of 40°C during 60min digestion period, which was lower ca. two magnitudes than that at 0°C and one magnitude than that at 25°C.
2688 related Products with: Heating enhanced sensitive and selective electrochemical detection of Hgbased on T-Hg-T structure and exonuclease III-assisted target recycling amplification strategy at heated gold disk electrode.Beta Amyloid (1 42) High 3-O-Acetyl-17-O-tert-buty Androsta-1,4,6-triene-3,1 (3β)-Androsta-5,16-diene Androst-4-ene-3,6,17-trio Androsta-3,5,16-trien-17- (5α)-Androstane-3,11,17- Rabbit Anti-ANT-1 ATP car Bovine Androstenedione,AS Goat Anti-Human Androgen Rabbit Anti-Rat Androgen EnzyChrom™ NAD NADH Ass
#29306030 // Save this To Up
Exonuclease III-boosted cascade reactions for ultrasensitive SERS detection of nucleic acids.A variety of nucleic acid amplification techniques have been integrated into different detection methods to promote the development of sensitive and convenient analysis of nucleic acids. However, it is still in urgent need to develop amplified nucleic acid biosensors for the analysis of susceptible gene and even distinguishing single-base mismatched DNA in complex biological samples. Benefiting from the achieved detection strategies, here we boost isothermal nucleic acid amplification by resorting to enzyme amplification, and combine this two-stage amplification method with surface-enhanced Raman spectroscopy (SERS) to develop a signal-on nucleic acid detection platform. Due to the high cleavage efficiency of Exonuclease III (Exo III), a large amount of trigger DNA are produced to initiate multiple hybridization chain reaction circles. The product structure tagged with Tamra is then anchored onto the plasmonic SERS substrate and meanwhile enriched. It is demonstrated that this detection platform is sensitive toward the myocardial infarction disease related gene. A detection limit of 1 fM for the gene analysis in a linear relationship in the wide range from 1 fM to 10nM is achieved, better than most of previous counterparts. Meanwhile, our developed detection platform exhibits a high selectivity for the target gene over mismatched analogues. Our strategy provides a robust tool for signal amplification of gene detection even in blood samples.
2297 related Products with: Exonuclease III-boosted cascade reactions for ultrasensitive SERS detection of nucleic acids.Anti AGE 3 Monoclonal Ant Formaldehyde Detection Ki MarkerGeneTM Fluorescent Cellufine Formyl , 50 ml Cellufine Formyl Media Cellufine Formyl , 500 ml Cellufine Formyl Media Cellufine Formyl Media Formalin Solution (20%) Formalin Solution (20%) Formalin Solution (20%) Formalin (10% Neutral Bu
#29239600 // Save this To Up
Exonuclease III-Assisted Upconversion Resonance Energy Transfer in a Wash-Free Suspension DNA Assay.Sensitivity is the key in optical detection of low-abundant analytes, such as circulating RNA or DNA. The enzyme Exonuclease III (Exo III) is a useful tool in this regard; its ability to recycle target DNA molecules results in markedly improved detection sensitivity. Lower limits of detection may be further achieved if the detection background of autofluorescence can be removed. Here we report an ultrasensitive and specific method to quantify trace amounts of DNA analytes in a wash-free suspension assay. In the presence of target DNA, the Exo III recycles the target DNA by selectively digesting the dye-tagged sequence-matched probe DNA strand only, so that the amount of free dye removed from the probe DNA is proportional to the number of target DNAs. Remaining intact probe DNAs are then bound onto upconversion nanoparticles (energy donor), which allows for upconversion luminescence resonance energy transfer (LRET) that can be used to quantify the difference between the free dye and tagged dye (energy acceptor). This scheme simply avoids both autofluorescence under infrared excitation and many tedious washing steps, as the free dye molecules are physically located away from the nanoparticle surface, and as such they remain "dark" in suspension. Compared to alternative approaches requiring enzyme-assisted amplification on the nanoparticle surface, introduction of probe DNAs onto nanoparticles only after DNA hybridization and signal amplification steps effectively avoids steric hindrance. Via this approach, we have achieved a detection limit of 15 pM in LRET assays of human immunodeficiency viral DNA.
1775 related Products with: Exonuclease III-Assisted Upconversion Resonance Energy Transfer in a Wash-Free Suspension DNA Assay.DNA (cytosine 5) methyltr Amplite™ Fluorimetric H Amplite™ Intracellular Amplite™ Fluorimetric P Amplite™ Fluorimetric A Cell Meter™ Intracellul Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri Triglyceride Assay Kit Li Alkaline Phospatase (ALP) MMP-13 inhibitor assay ki MMP13 inhibitor assay kit
#29171254 // Save this To Up
Near-Infrared-to-Ultraviolet Light-Mediated Photoelectrochemical Aptasensing Platform for Cancer Biomarker Based on Core-Shell NaYF:Yb,Tm@TiOUpconversion Microrods.Titanium dioxide (TiO; as a potential photosensitizer) has good photocurrent performance and chemical stability but often exhibits low utilization efficiency under ultraviolet (UV) region excitation. Herein, we devised a near-infrared light-to-UV light-mediated photoelectrochemical (PEC) aptasensing platform for the sensitive detection of carcinoembryonic antigen (CEA) based on core-shell NaYF:Yb,Tm@TiOupconversion microrods by coupling with target-triggered rolling circle amplification (RCA). The upconversion microrods synthesized through the hydrothermal reaction could act as a photosensing platform to convert the near-infrared (near-IR) excitation into UV emission for generation of photoinduced electrons. The target analyte was determined on a functional magnetic bead by using the corresponding aptamers with a sandwich-type assay format. Upon target CEA introduction, a complex was first formed between capture aptamer-1-conjugated magnetic bead (Apt1-MB) and aptamer-2-primer DNA (Apt2-pDNA). Thereafter, the carried primer DNA by the aptamer-2 paired with linear padlock DNA to trigger the RCA reaction. The guanine (G)-rich product by RCA reaction was cleaved by exonuclease I and exonuclease III (Exos I/III), thereby resulting in the formation of numerous individual guanine bases to enhance the photocurrent of core-shell NaYF:Yb,Tm@TiOupconversion microrods under near-IR illumination (980 nm). Under optimal conditions, the near-IR light-mediated PEC aptasensing system could exhibit good photoelectrochemical response toward target CEA and allowed for the detection of target CEA as low as 3.6 pg mL. High reproducibility and good accuracy were achieved for analysis of human serum specimens. Importantly, the near-IR-activated PEC aptasensing scheme provides a promising platform for ultrasensitive detection of other biomolecules.
1620 related Products with: Near-Infrared-to-Ultraviolet Light-Mediated Photoelectrochemical Aptasensing Platform for Cancer Biomarker Based on Core-Shell NaYF:Yb,Tm@TiOUpconversion Microrods.Colon cancer high density High density larynx and p MarkerGeneTM Fluorescent Multiple cancer tissue ar High density (208 core) p Rectum cancer high densit Skin cancer and normal ti Small intestine cancer, m Stomach cancer and normal Top 4 types of cancer (co Cellufine Formyl , 50 ml Cellufine Formyl Media
#29159353 // Save this To Up
Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling.One of the promising approaches to address the challenge of detecting dilute nucleic acid analytes is exonuclease III-aided target recycling. In this strategy, the target DNA self-assembles with the reactant DNA probes and displays itself as a reactant and product at the same time. This provides an autonomous mechanism to release and reuse the analyte from each round of reactions for repetitive cycles, which amplifies the signal without amplifying the analyte itself. However, for very low amounts of the analyte, it takes a considerably long time before a detectable signal is generated. Thus, in this paper, we report a kinetically-enhanced target recycling strategy by designing two more target recycling sub-reactions that are triggered by the byproducts of the first reaction involving the target analyte. In this manner, concentrations of up to 0.5 pM of target DNA can be detected in 15 minutes.
1900 related Products with: Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling.Enhanced Apoptotic DNA La ACTGene Blue Hot Start DN Quick Apoptotic DNA Ladde MarkerGene™ Biotin Dete Multiple cancer tissue ar Protease, DNASE free hea Protease, DNASE free hea Protease, DNASE free hea Nuclear Fast Red Solutio Nuclear Fast Red Solutio Nuclear Fast Red Solutio Primary Antibody Dropper
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia