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Collagen-Induced Arthritis Mouse Model.

The collagen-induced arthritis mouse model is a widely studied autoimmune model of rheumatoid arthritis. In this model, autoimmune arthritis is induced by immunization of genetically susceptible strains of mice with type II collagen emulsified in complete Freund's adjuvant. This article describes the steps necessary for the acquisition, handling, and preparation of CII, in addition to the selection of mouse strains, proper immunization technique, and methods for evaluation of the incidence and severity of the autoimmune arthritis. In this model, the first signs of arthritis appear approximately 21 to 28 days after immunization. The protocols in this article should provide the investigator with all the necessary information required to reproducibly induce a high incidence of CIA in genetically susceptible strains of mice, and to critically evaluate the pathology of the disease. Published 2021. This article is a U.S. Government work and is in the public domain in the USA. Basic Protocol: Induction of collagen-induced arthritis Support Protocol 1: Purification of type II collagen Support Protocol 2: Purification of type II collagen α1(II) chains Support Protocol 3: Assessment of arthritis incidence and severity Support Protocol 4: Measurement of CII specific antibody by indirect ELISA Support Protocol 5: Coupling CII to magnetic beads Support Protocol 6: Measuring CII-specific antibody by magnetic-bead based ELISA Support Protocol 7: Measurement of T cell responses to CII in CIA.
Edward F Rosloniec, Karen Whittington, Amanda Proslovsky, David D Brand

1858 related Products with: Collagen-Induced Arthritis Mouse Model.

2ug0.1 ml

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#26790721   2016/01/11 To Up

Evidence for enhanced collagen type III deposition focally in the territorial matrix of osteoarthritic hip articular cartilage.

To determine if type III collagen is concentrated in the chymotrypsin-extractable collagen pool from osteoarthritic articular cartilage to assess its potential as a biomarker of Osteoarthritis (OA) pathogenic mechanisms.
S Hosseininia, M A Weis, J Rai, L Kim, S Funk, L E Dahlberg, D R Eyre

1427 related Products with: Evidence for enhanced collagen type III deposition focally in the territorial matrix of osteoarthritic hip articular cartilage.

100 μg100 μg0.5 mg5 mg lyophilized

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#16162901   // To Up

Are there autoantibodies reacting against citrullinated peptides derived from type I and type II collagens in patients with rheumatoid arthritis?

To assess the possible presence in patients with rheumatoid arthritis (RA) of autoantibodies recognising citrullinated peptides derived from type I and II collagens.
M-K Koivula, S Aman, A Karjalainen, M Hakala, J Risteli

1680 related Products with: Are there autoantibodies reacting against citrullinated peptides derived from type I and type II collagens in patients with rheumatoid arthritis?

96T1 mL96T10 96 wells (1 kit)1 mg1 mL1 mg96 wells (1 kit)

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#15637808   // To Up

The assessment of cartilage degradation in vivo: development of an immunoassay for the measurement in body fluids of type II collagen cleaved by collagenases.

A monoclonal antibody has been developed which recognizes a neoepitope in type II collagen which is generated by the intrahelical cleavage of collagenases. Antibody reactivity is directed at the carboxyl-terminus of the TCA or 3/4 piece of the degraded alpha1(II) chain. Reactivity is dependent upon hydroxylation of proline. Evidence is provided suggesting that epitope binding involves the recognition of a conformational neoepitope. Using an ELISA, we show that this neoepitope can be detected in the urines and sera of nonarthritic persons and patients with rheumatoid arthritis (RA). An increased content is observed in the sera and urines of patients. The assay may be of value in studying cartilage type II degradation both in vitro and in vivo such as in those with arthritis.
A Robin Poole, Mirela Ionescu, M A Fitzcharles, R Clark Billinghurst

1635 related Products with: The assessment of cartilage degradation in vivo: development of an immunoassay for the measurement in body fluids of type II collagen cleaved by collagenases.

100 μg100 μg16 Arrays/Slide100.00 ug48 samples

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