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#34072200   2021/05/27 To Up

Molecular Epidemiology of Human Papillomaviruses, and among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes.

Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit "HPV Genotypes 14 Real-TM Quant" from SACACE Biotechnologies, detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the real-time multiplex amplification kit /// Real-TM, allowing their simultaneous detection, was used. The women were aged 17-50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), (13.74%), (11.54%) and (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were   24.00%,   22.00%,   17.00%, 13.00% and   1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers of West and Central African origin working in Ouagadougou.
Sessi Frida Tovo, Théodora Mahoukèdè Zohoncon, Amana Metuor Dabiré, Régine Ilboudo, Rahimatou Yasmine Tiemtoré, Dorcas Obiri-Yeboah, Albert Théophane Yonli, Essi Etonam Dovo, Rogomenoma Alice Ouédraogo, Abdoul Karim Ouattara, Pegdwende Abel Sorgho, Djénéba Ouermi, Florencia Wendkuuni Djigma, Charlemagne Ouédraogo, Lassana Sangaré, Jacques Simpore

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