Search results for: Bottles 500 ml PPCO_FB with screw cap
#25049465 
2014/05/16
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In vitro propagation of female Ephedra foliata Boiss. & Kotschy ex Boiss.: an endemic and threatened Gymnosperm of the Thar Desert.
Ephedra foliata Boiss. & Kotschy ex Boiss., (family - Ephedraceae), is an ecologically and economically important threatened Gymnosperm of the Indian Thar Desert. A method for micropropagation of E. foliata using nodal explant of mature female plant has been developed. Maximum bud-break (90 %) of the explant was obtained on MS medium supplemented with 1.5 mg l(-1) of benzyl adenine (BA) + additives. Explant produces 5.3 ± 0.40 shoots from single node with 3.25 ± 0.29 cm length. The multiplication of shoots in culture was affected by salt composition of media, types and concentrations of plant growth regulators (PGR's) and their interactions, time of transfer of the cultures. Maximum number of shoots (26.3 ± 0.82 per culture vessel) were regenerated on MS medium modified by reducing the concentration of nitrates to half supplemented with 200 mg l(-1) ammonium sulphate {(NH4) 2SO4} (MMS3) + BA (0.25 mg l(-1)), Kinetin (Kin; 0.25 mg l(-1)), Indole-3-acetic acid (IAA; 0.1 mg l(-1)) and additives. The in vitro produced shoots rooted under ex vitro on soilrite moistened with one-fourth strength of MS macro salts in screw cap bottles by treating the shoot base (s) with 500 mg l(-1) of Indole-3-butyric acid (IBA) for 5 min. The micropropagated plants were hardened in the green house. The described protocol can be applicable for (i) large scale plant production (ii) establishment of plants in natural habitat and (iii) germplasm conservation of this endemic Gymnosperm of arid regions.Deepika Lodha, Nisha Rathore, Vinod Kataria, N S Shekhawat
2784 related Products with: In vitro propagation of female Ephedra foliata Boiss. & Kotschy ex Boiss.: an endemic and threatened Gymnosperm of the Thar Desert.
96 tests100 μg50ul (1mg/ml)1 mL1
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#27446739 2002/10/01
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Stability of Standard Electrolytic Conductivity Solutions in Glass Containers.
The stability of solutions having an electrolytic conductivity, κ , of 5 μS/cm to 100 000 μS/cm packaged in glass screw-cap bottles, glass serum bottles, and glass ampoules was monitored for 1 year to 2 years. The conductivity was determined by measuring the ac resistance of the solution. Mass loss was also monitored for solutions packaged in bottles. The solutions were prepared using KCl in water ( κ ≥100 μS/cm) or KCl in 30 % (by mass) n-propanol 70 % (by mass) water ( κ ≤ 15 μS/cm). The conductivity changes were compared by packaging type and by nominal κ . The main causes of the κ changes are evaporation (screw-cap bottles) and leaching (screw-cap bottles, serum bottles, and ampoules). Evaporation is determined from mass loss data; leaching occurs from the glass container with no change in mass. The choice of optimal packaging, which depends on the conductivity level, is the packaging in which κ changes the least with time. Ampoules are the most suitable packaging for standards having nominal κ values of 500 μS/cm to 100 000 μS/cm. Screw-cap bottles are most suitable for standards having a nominal κ of 5 μS/cm to 100 μS/cm.Rubina H Shreiner