Gentaur Pub

#35646170   // To Up

The antimicrobial and antibiofilm effects of three herbal extracts on compared with Chlorhexidine 0.2% ( study).

There is a special focus on using natural materials and herbal plants to prevent dental caries. Previous studies showed that some herbal plants have antimicrobial effects on oral pathogens. Thus we investigated the antimicrobial effects of three herbal extracts (, , and ) on the growth of , as the most important bacteria causing dental caries. First, plant methanolic extracts were prepared. Then, to evaluate the antimicrobial activity of the three herbal extracts, the agar well diffusion method and MIC were performed. The biofilm formation was carried out using a broth dilution method with 2% glucose-supplemented BHIS in sterile 96-well microplates. Serial dilutions (50, 25, 12.5, 6.25, 3.12 mg/ml) of extracts were prepared. Next, a 0.5 McFarland Suspension of was added to wells. The inhibitory effect on biofilm formation was measured by the ELISA reader apparatus. The assay was repeated three times, and the average was calculated as 3. The results were compared with those of Chlorhexidine 0.2%. showed a better effect in the agar well diffusion method than others. MIC of the extracts of Carum coptimum, , and were 3.12, 6.25, and 12.5 mg/ml, respectively. Overall, the highest activity belonged to extract. For the anti-biofilm effect, the OD values of and were significantly different from that of . Although all of the methanolic herbal extracts can inhibit growth and remove the biofilm, the effect of was better than and . Further studies are recommended to indicate how these extracts perform against the bacteria.
Aida Mehdipour, Azita Ehsani, Nasrin Samadi, Marzieh Ehsani, Negar Sharifinejad

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1 mg100 U 2 ml 2.5 mg100.00 ul 100 G100ug Lyophilized1,000 tests200ug

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#29263627   // To Up

Inhibitory Effects of Bean Oil and Extract on .

is a tree of the Brazilian Savannah. The beans of this tree are used in folk medicine as anti-inflammatory preparations, especially for infections caused by . These bacteria can cause simple infections or serious illnesses such as pneumonia, meningitis, endocarditis, toxic shock syndrome, septicemia, and others.
V S Mendes, J B Sant'Anna, S C C Oliveira, Iriani Rodrigues Maldonade, Eleuza Rodrigues Machado

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100ug1000 TESTS/0.65ml100 reactions100ug 6 ml Ready-to-use 1mg100 extractions25 mg10 μg500 50 assays

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#24756630   2014/04/23 To Up

Evaluation of liquid and solid culture media for the recovery and enrichment of Burkholderia cenocepacia from distilled water.

Burkholderia cepacia complex (BCC) presence has been the cause of recalls of both sterile and non-sterile pharmaceutical products since these opportunistic pathogens have been implicated to cause infections to susceptible individuals. BCC are ubiquitous in nature, but in pharmaceutical settings the most common source is contaminated water systems. Some strains of BCC, previously described as Pseudomonas cepacia, were not readily detected by standard culture methods. We have explored different strategies to recover and enrich Burkholderia cenocepacia previously cultured in distilled water for 40 days. Enrichment media of varied nutrient concentrations and composition were used, including modified Tryptic Soy Agar or Broth (TSA or TSB), Reasoner's 2nd Agar or Broth (R2A or R2AB), Brain-Heart Infusion Broth (BHIB), Mueller-Hinton Broth (MHB), and Ashdown's (ASH) medium. Of the various broth media tested, cell growth was significantly greater in TSB and R2AB than in BHIB, MHB, or ASH broth. TSB and R2AB were also compared for their recovery efficiency. Generally, there was no significant difference between the numbers of B. cenocepacia grown on 15 differently modified TSA and five modified R2A solid media. Overall, however, diluted TSA and TSB media, and R2A and R2AB showed better recovery efficiency than TSA and TSB for inocula containing small numbers of cells. All strains persisted in distilled water for 40 days. Broth media were more effective than solid media for recovery of B. cenocepacia from distilled water. These results may assist in improving detection assays with recovery and enrichment strategies to maximize recovery of these fastidious organisms.
Youngbeom Ahn, Jeong Myeong Kim, Hyeri Ahn, Yong-Jin Lee, John J LiPuma, David Hussong, Carl E Cerniglia

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2.5 mg500 grams10 reactions10 mg50 ml 5 G1,000 tests100ul1 mg 5 lt2.5 mg1 g

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