Search results for: Anti_Human CD1a_PE conjugate
#26514172 2016/01/05 Save this To Up
Immuno-PET Imaging of CD30-Positive Lymphoma Using 89Zr-Desferrioxamine-Labeled CD30-Specific AC-10 Antibody.The CD30-specific antibody-drug conjugate, brentuximab vedotin, is approved for the treatment of relapsed, refractory Hodgkin lymphomas and systemic anaplastic large T-cell lymphomas. Multiple ongoing clinical trials are investigating brentuximab vedotin efficacy in other CD30-positive hematologic malignancies. Because CD30 expression varies among different types of lymphoma and can also change during the course of treatment, companion diagnostic imaging of CD30 could be a valuable tool in optimizing patient-specific brentuximab vedotin treatment regimens.
1452 related Products with: Immuno-PET Imaging of CD30-Positive Lymphoma Using 89Zr-Desferrioxamine-Labeled CD30-Specific AC-10 Antibody.PABP1-dependent poly A-sp Anti-acetyl Histone H3 (A Anti acetyl Histone H3 (A Anti acetyl Histone H3 (A Anti-Acetyl Histone H3 (A Anti Acetyl Histone H3 (A Anti Acetyl Histone H3 (A MMP (Human) Antibody Arra Rabbit Anti-PET2 CDC73 Pa Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia Rabbit Anti-Nkx2.5 Cardia
#26329478 2015/11/20 Save this To Up
Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections.In this study, a lateral flow immunochromatographic assay (LFIA) system for the detection of immunoglobulin M (IgM) antibodies, related to TORCH [(T)oxoplasmosis, (O)ther agents, (R)ubella (also known as German Measles), (C)ytomegalovirus, and (H)erpes simplex virus infections], based on gold magnetic nanoparticles, was established. Following modification with poly(methacrylic acid), the gold magnetic nanoparticles conjugated with an anti‑human IgM antibody (μ‑chain specific) to construct a probe. A lateral flow assay device was constructed based on these conjugates. IgM antibodies to four types of pathogens, notably toxoplasmosis, rubella virus, cytomegalovirus and herpes simplex virus type 2, were detected using this device. Compared with commercial colloidal gold‑based LFIA strips, our method exhibited higher sensitivity. No interference with triglycerides, hemoglobin and bilirubin occurred, and no cross‑reactivity was noted among the four pathogens. The gold magnetic nanoparticle‑LFIA strips were used to assess 41 seropositive and 121 seronegative serum samples. The sensitivity was 100% (162/162) and the specificity was 100% (162/162). This method cannot only be used for the detection of TORCH IgM-specific antibodies, but it can potentially be developed for use in the diagnosis of other acute or recently identified autoimmune diseases.
2809 related Products with: Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections.MarkerGeneTM Fluorescent LATERAL FLOW ASSAY EZ PAN TORCH IgM screening (capt EpiQuik Total Histone H EpiQuik Total Histone H EpiQuik Total Histone H EpiQuik Total Histone H Shiga Toxin 1 antibody, M Shiga Toxin 2 antibody, M Human IgM antibody, Monoc FITC antibody, Monoclonal ACE antibody, Monoclonal
#24659029 2014/07/21 Save this To Up
A new Eu(3+)-labeled method for anticardiolipin antibody IgM.The anticardiolipin antibodies (aCL) test has become a laboratory standard for the clinical diagnosis of antiphospholipid syndrome (APS). To better the quantitative detection of aCL-IgM so as to classify patients correctly and timely as APS positive, we established herein a new immunoassay based on a time-resolved fluoroimmunoassay (TRFIA).
MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI BOVINE ROTAVIR Human IgM antibody, Monoc FITC antibody, Monoclonal ACE antibody, Monoclonal Adenovirus antibody, Mono CA 50 antibody, Monoclona Campylobacter jejuni anti DNA antibody, Monoclonal Gram Negative Endotoxin a HBsAg antibody, Monoclona
#22684164 2012/10/11 Save this To Up
Detection of anticardiolipin antibody IgG by time-resolved fluoroimmunoassay.In an effort to improve the quantitative detection of anticardiolipin antibodies (aCL) IgG so as to classify patients correctly as antiphospholipid syndrome (APS) positive, we developed a new immunoassay based on a sandwich time-resolved fluoroimmunoassay (TRFIA) using the complex of cardiolipin plus bovine β(2)GPI as antigen and Eu(3+)-labeled rabbit antihuman IgG as conjugate. The precision, sensitivity, specificity, and stability of the assay were evaluated, and comparison with the classical ELISA was also made. The aCL IgG TRFIA kit we established had a wider detectable range than three commercial ELISA ones from different manufacturers when a specimen was diluted, with strong positive result from 1:12.5 to 1:204,800. The average intra-assay and inter-assay CVs detected by the aCL IgG TRFIA was 3.14 and 3.70 %, respectively. The sensitivity was 0.1 GPL U/ml, and the clinical diagnostic specificity was 98 %. The established assay kit also behaved better in stability than the commercial ELISA ones. Additionally, the immunoassay we established correlated well with the ELISA, and the correlation coefficient was 0.975. We thus conclude that the TRFIA we developed for aCL IgG detection gives promise to a more sensitive and reliable diagnosis of APS and has potential value for large-scale screening programs.
1937 related Products with: Detection of anticardiolipin antibody IgG by time-resolved fluoroimmunoassay.B-Phycoerythrin antibody 2-Amino Benzimidazole Su 2-Amino Benzimidazole Su Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti CRC3 CD3 (bispecific) Cl 2,3 dinor 6 keto Prostag ROR1 Clone '1B4 antibody RBPMS HERMES Clone '1C12 MOUSE ANTI BOVINE ROTAVIR Detection Buffer A&B Anti Detection Buffer C&D Anti
#21897268 2011/12/07 Save this To Up
Immunofluorescence-detected infiltration of CD4+FOXP3+ regulatory T cells is relevant to the prognosis of patients with endometrial cancer.Host antitumor immune responses are associated with many types of immune cells and soluble components. In particular, CD8 cytotoxic T lymphocytes (CTLs) play a central role. Regulatory T cells (Tregs) have been reported to induce tumor immune tolerance in various cancers. In the present study, we evaluated lymphocytic infiltration in endometrial cancer tissue to clarify its relationship with clinicopathological factors and the prognosis of patients.
1652 related Products with: Immunofluorescence-detected infiltration of CD4+FOXP3+ regulatory T cells is relevant to the prognosis of patients with endometrial cancer.Shiga Toxin 1 antibody, M Shiga Toxin 2 antibody, M Cholera toxin antibody, M Clostridium botulinum D T Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Clostridum difficile toxi Diphtheria toxin antibody
#20979380 2010/11/18 Save this To Up
Quantitative detection of single molecules using enhancement of Dye/DNA conjugate-labeled nanoparticles.An ultrasensitive fluorescence immunoassay method for quantitative detection of single molecules is developed on the basis of counting single magnetic nanobeads (MNBs) with combined amplification of DNA and dye/DNA conjugate. Highly amplified fluorescence signal and low background signal are achieved by using mutilabel bioconjugates made by linking multiple dye/DNA conjugates to streptavidin-coated magnetic nanobeads (SA-MNBs) and magnetic separation. In this method, human IgG (Ag) is captured on the silanized glass substrate surface, followed by immunoreaction with biotinylated mouse antihuman antibody (BT-Ab). Then, SA-MNBs are attached to the BT-Ab through the biotin/streptavidin interaction at a ratio of 1:1. Subsequently, a 30 base pair double-stranded oligonucleotide terminated with biotin (BT-dsDNA) is conjugated to the SA-MNBs. The resultant Ag-BT-Ab-SA-MNBs/BT-dsDNA/SYBR Green I is achieved after a fluorescent DNA probe, SYBR Green I, is added to the substrate and bound to the oligonucleotide at high ratios. Finally, epifluorescence microscopy coupled with a high-sensitivity electron multiplying charge-coupled device is employed for human IgG fluorescence imaging and detection. The number of fluorescent spots corresponding to single protein molecules on the images is counted. It is found that the number of fluorescent spots resulting from the SA-MNBs/BT-dsDNA/SYBR Green I immuotargeted on the glass slides is correlated with the concentration of human IgG target antigen in the range 3.0-50 fM.
2749 related Products with: Quantitative detection of single molecules using enhancement of Dye/DNA conjugate-labeled nanoparticles.E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran Taq SSB (Single Stranded Taq SSB (Single Stranded EIA for Quantitative Dete EIA for Quantitative Dete Beta Amyloid (40) ELISA K Beta Amyloid (1 42) ELISA Single Strand DNA Ligase, Single Strand DNA Ligase,
#20348406 2010/04/29 Save this To Up
Gamma' fibrinogen: evaluation of a new assay for study of associations with cardiovascular disease.Studies of disease associations with gamma' fibrinogen, a newly emerging risk factor for cardiovascular disease, have been hampered by the lack of a standardized and well-characterized assay.
1287 related Products with: Gamma' fibrinogen: evaluation of a new assay for study of associations with cardiovascular disease.Fibrinogen gamma antibody Glucose Assay With the La Cultrex In Vitro Angiogen Endothelial Tube Formatio Cardiovascular disease ti Cardiovascular disease ti QuantiChrom™ Formaldehy QuantiChrom™ Formaldehy Mouse Anti-Newcastle Dise Formate Assay Kit Gamma Glutamyl Transferas Lymphoma array, together
#19159096 2009/01/22 Save this To Up
Electrochemical immunosensing using micro and nanoparticles.A model immunosensor based on a labeling method using gold nanoparticles (AuNPs) and electrochemical detection is developed. Microparamagnetic beads (MB) as primary antibody immobilization platforms and AuNPs modified with a secondary antibody as high sensible electrochemical labels have been used. The carbon electrode used as transducer incorporates a magnet that allows the collection/ immobilization on its surface of the immunological sandwich attached to the MB. Briefly, the sandwich type assay consists in the incubation of streptavidin-coupled-MB with an antihuman IgG biotin conjugate, and then, the immunological reaction with the human IgG antigen takes place. After that, a gold labeled anti-human IgG reacts with the antigen, and finally the AuNPs are electrochemically detected. This approach allows the obtaining of an immunosensor with a low antigen detection limit with special interest for several applications in protein analysis.
Strep Tactin(R) Coated M Rapid Microplate Assay K Fraction V Microbiologic Fraction V Microbiologic Fraction V Microbiologic Fraction V Microbiologic Copper Stain Kit (For Mi Copper Stain Kit (For Mi PTAH Stain Kit for Micro PTAH Stain Kit for Micro Sterile filtered goat se Sterile filtered goat se
#15627622 2005/01/03 Save this To Up
Development and validation of ELISA for herceptin detection in human serum.Herceptin, a humanized anti-human epidermal growth factor receptor-2 (HER2) monoclonal antibody, is used for treatment of metastatic breast cancer patients overexpressing HER2 on tumor cells, and is being studied in clinical trials for therapy of other types of cancer. In the present work, we developed a Herceptin enzyme-linked immunosorbent assay (ELISA) from commercially available reagents to meet the growing needs of clinical studies. In this immunoassay, a mixture of monoclonal antibodies specific for the cytoplasmic domain of human HER2 (676-1255 amino acids) is adsorbed onto a microtiter plate, followed by addition of full-length HER2 protein, which is captured by the antibodies. Herceptin in the serum that is added to the microwells binds to the extracellular domain (ECD) of the captured HER2. The Herceptin bound to HER2 is detected by an antihuman IgG-horse radish peroxidase (HRP) conjugate and a (3, 5, 3', 5')-tetramethylenbenzidine (TMB) substrate. The calibration range of the assay is 5-100 ng/mL after 1:2000 sample dilution corresponding to 10-200 microg/mL Herceptin in undiluted serum. The intra- and interassay CVs ranged from 4.56% to 13.3% and from 9.9% to 18.9%, respectively. The assay shows dilutional linearity and specificity. Soluble p105 HER2, which can be shed into serum does not interfere with the assay. The analytical performance of the Herceptin ELISA indicates that this assay can be used for monitoring concentration levels of Herceptin in human serum.
1539 related Products with: Development and validation of ELISA for herceptin detection in human serum.Human interleukin 2(IL-2) Beta Amyloid (42) ELISA K Beta Amyloid (1 40) ELISA Beta Amyloid (40) ELISA K Beta Amyloid (40) ELISA K Beta Amyloid (1 42) High Beta Amyloid (1 42) ELISA Beta Amyloid (1 40) ELISA Aldosterone ELISA Human S ELISA Human Serum B2 Micr C Reactive Protein (hs CR Dehydroepiandrosterone Su
#12754109 2003/05/19 Save this To Up
Delivery of pharmacologically active dexamethasone into activated endothelial cells by dexamethasone-anti-E-selectin immunoconjugate.To deliver selectively anti-inflammatory agents into activated endothelial cells, drug-targeting conjugates were developed. Dexamethasone (Dexa) was covalently linked to a monoclonal antibody specifically recognizing E-selectin, which is strongly upregulated in endothelial cells at inflammatory sites. In the present study, the pharmacological effects of this Dexa-mouse antihuman E-selectin antibody (H18/7) (Ab(hEsel)) conjugate were investigated and compared to the effects obtained by free Dexa in human umbilical vein endothelial cells. Flow cytometry and ELISA were performed to analyze the levels of cell adhesion molecules (ICAM-1 and VCAM-1) and secreted cytokines (IL-6 and IL-8). The studies were extended by analysis of a complex gene expression pattern, using a cDNA expression array containing 268 genes encoding human cytokines/cytokine-receptors. Fifty genes and 28 genes were upregulated (ratio> or =2) upon incubation of human umbilical vein endothelial cells with TNFalpha for 6 and 24hr, respectively. This gene expression profile was markedly altered when cells were activated with TNFalpha in the presence of Dexa (100 nM) or Dexa-Ab(hEsel) conjugate (10 micro g/mL conjugate corresponding to 100 nM Dexa). Relative and competitive RT-PCR analysis verified downregulation of TNFalpha-mediated expression of CD40L and IL-8 by Dexa and Dexa-Ab(hEsel), respectively. These results indicated a successful internalization and processing of Dexa-Ab(hEsel) in activated endothelial cells, allowing the intracellularly delivered Dexa to exert its pleiotropic anti-inflammatory activity.
1037 related Products with: Delivery of pharmacologically active dexamethasone into activated endothelial cells by dexamethasone-anti-E-selectin immunoconjugate.anti CD38 Hematopoietic p anti Transferrin receptor Mouse Anti-Human Endothel Mouse Anti-Pig Endothelia Rat Anti-Human Endothelia Mouse Anti-Human Endothel Rabbit Anti-Dexamethasone Anti C Reactive Protein A anti HSV (II) gB IgG1 (mo anti HCMV IE pp65 IgG1 (m anti HCMV gB IgG1 (monocl Epidermal Growth Factor (
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