Only in Titles

           Search results for: Alleleustrious pmWasabi_Mitochondria fusion vector   

paperclip

#29694213   // Save this To Up

Erratum: Fully Differential Vector-Boson-Fusion Higgs Production at Next-to-Next-to-Leading Order [Phys. Rev. Lett. 115, 082002 (2015)].

This corrects the article DOI: 10.1103/PhysRevLett.115.082002.

2542 related Products with: Erratum: Fully Differential Vector-Boson-Fusion Higgs Production at Next-to-Next-to-Leading Order [Phys. Rev. Lett. 115, 082002 (2015)].

pNosdcGUS Plant GUS Expre pCAMBIA1381Xa Vector (Fus pCAMBIA1381Xb Vector (Fus pCAMBIA1381Xc Vector (Fus ATF3 FOXO3A (GFP Fusion Tag) Human Beta-cell Attractin REASTAIN® Quick Diff Kit Caspase-12 Inhibitor Z-AT Caspase-12 Inhibitor Z-AT Caspase 12 Inhibitor Z AT Caspase-12 Inhibitor Z-AT

Related Pathways

  •  
  • No related Items
paperclip

#29673256   // Save this To Up

A novel, easy and rapid method for constructing yeast two-hybrid vectors using In-Fusion technology.

Yeast two-hybrid systems are powerful tools for analyzing interactions between proteins. Vector construction is an essential step in yeast two-hybrid experiments, which require bait and prey plasmids. In this study, we modified the multiple cloning site sequence of the yeast plasmid pGADT7 by site-directed mutagenesis PCR to generate the pGADT7-In vector, which result in an easy and rapid method for constructing yeast two-hybrid vectors using the In-Fusion cloning technique. This method has three key advantages: only one pair of primers and one round of PCR are needed to generate bait and prey plasmids for each gene; it is restriction endonuclease- and ligase-independent and it is fast and easily performed.

2596 related Products with: A novel, easy and rapid method for constructing yeast two-hybrid vectors using In-Fusion technology.

QuantiChrom™ Formaldehy EnzyChrom™ Kinase Assay MarkerGeneTM Fluorescent Rapid Microplate Assay K Adeno Lenti EGFP (hybrid) Anti C Reactive Protein A MOUSE ANTI BOVINE ROTAVIR Bone Morphogenetic Protei Growth Differentiation Fa Amplite™ Fluorimetric F Confocal Dish,PS,clear, 3 Androgen Receptor (Phosph

Related Pathways

paperclip

#29659495   // Save this To Up

Transient Recombinant Protein Production in Glycoengineered Cell Suspension Culture.

Transient recombinant protein production is a promising alternative to stable transgenic systems, particularly for emergency situations in which rapid production of novel therapeutics is needed. In plants, can be used as a gene delivery vector for transient expression. A potential barrier for plant-based production of human therapeutics is that different glycosylation patterns are found on plant and mammalian proteins. Since glycosylation can affect the efficacy, safety and stability of a therapeutic protein, methods to control glycan structures and distributions in plant-based systems would be beneficial. In these studies, we performed -mediated transient expression in glycoengineered plant cell suspension cultures. To reduce the presence of plant-specific glycans on the product, we generated and characterized cell suspension cultures from β-1,2-xylosyltransferase and α-1,3-fucosyltransferase knockdown . An anthrax decoy fusion protein was transiently produced in these glycoengineered plant cell suspension cultures through co-culture with genetically engineered . The mass ratio of to plant cells used was shown to impact recombinant protein expression levels. N-glycosylation analysis on the anthrax decoy fusion protein produced in glycoengineered showed a dramatic reduction in plant-specific N-glycans. Overall, the results presented here demonstrate the feasibility of a simple, rapid and scalable process for transient production of recombinant proteins without plant-specific glycans in a glycoengineered plant cell culture host.

1494 related Products with: Transient Recombinant Protein Production in Glycoengineered Cell Suspension Culture.

Macrophage Colony Stimula Macrophage Colony Stimula Recombinant Influenza HA Recombinant Influenza HA Recombinant Influenza HA Recombinant HBsAg adr [fr Recombinant HBsAg adr [fr Recombinant HBsAg adr [fr Recombinant Human HGF [fr Recombinant Human HGF [fr Recombinant Human HGF [fr Recombinant HIV-1 pol Int

Related Pathways

paperclip

#29641390   // Save this To Up

Simultaneous Recognition and Assessment of Post-Stroke Hemiparetic Gait by Fusing Kinematic, Kinetic, and Electrophysiological Data.

Gait analysis for the patients with lower limb motor dysfunction is a useful tool in assisting clinicians for diagnosis, assessment, and rehabilitation strategy making. Implementing accurate automatic gait analysis for the hemiparetic patients after stroke is a great challenge in clinical practice. This study is to develop a new automatic gait analysis system for qualitatively recognizing and quantitatively assessing the gait abnormality of the post-stroke hemiparetic patients. Twenty-one post-stroke patients and twenty-one healthy volunteers participated in the walking trials. Three of the most representative gait data, i.e., marker trajectory (MT), ground reaction force (GRF), and electromyogram, were simultaneously acquired from these subjects during their walking. A multimodal fusion architecture is established by using these different modal data to qualitatively distinguish the hemiparetic gait from normal gait by different pattern recognition techniques and to quantitatively estimate the patient's lower limb motor function by a novel probability-based gait score. Seven decision fusion algorithms have been tested in this architecture, and extensive data analysis experiments have been conducted. The results indicate that the recognition performance and estimation performance of the system become better when more modal gait data are fused. For the recognition performance, the random forest classifier based on the GRF data achieves an accuracy of 92.26% outperformed other single-modal schemes. When combining two modal data, the accuracy can be enhanced to 95.83% by using the support vector machine (SVM) fusion algorithm to fuse the MT and GRF data. When integrating all the three modal data, the accuracy can be further improved to 98.21% by using the SVM fusion algorithm. For the estimation performance, the absolute values of the correlation coefficients between the estimation results of the above three schemes and the Wisconsin gait scale scores for the post-stroke patients are 0.63, 0.75, and 0.84, respectively, which means the clinical relevance becomes more obvious when using more modalities. These promising results demonstrate that the proposed method has considerable potential to promote the future design of automatic gait analysis systems for clinical practice.

1496 related Products with: Simultaneous Recognition and Assessment of Post-Stroke Hemiparetic Gait by Fusing Kinematic, Kinetic, and Electrophysiological Data.

Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Androgen Receptor (Ab 650 AZD-3514 Mechanisms: Andr 17β-Acetoxy-2α-bromo-5 (5α,16β)-N-Acetyl-16-[2 (5α,16β)-N-Acetyl-16-ac 5α-N-Acetyl-2'H-androst- 5α-N-Acetyl-2'H-androst- 3-O-Acetyl 5,14-Androstad

Related Pathways

  •  
  • No related Items
paperclip

#29632241   // Save this To Up

A fragment of adhesion molecule L1 is imported into mitochondria and regulates mitochondrial metabolism and trafficking.

The cell adhesion molecule L1 plays important roles in the mammalian nervous system under physiological and pathological conditions. We have previously reported that proteolytic cleavage of L1 by myelin basic protein leads to the generation of a 70 kDa transmembrane L1 fragment (L1-70) that promotes neuronal migration and neuritogenesis. Here, we provide evidence that L1-70 is imported from the cytoplasm into mitochondria. Genetic ablation of L1, inhibition of mitochondrial import of L1-70 or prevention of myelin basic protein-mediated generation of L1-70 lead to reduced mitochondrial complex I activity, impaired mitochondrial membrane potential, fusion, fission, and motility as well as increased retrograde transport. We identified NADH dehydrogenase ubiquinone flavoprotein 2 as a binding partner for L1, suggesting that L1-70 interacts with this complex I subunit to regulate complex I activity. The results of our study provide insights into novel functions of L1 in mitochondrial metabolism and cellular dynamics. These functions are likely to ameliorate the consequences of acute nervous system injuries and chronic neurodegenerative diseases.

2678 related Products with: A fragment of adhesion molecule L1 is imported into mitochondria and regulates mitochondrial metabolism and trafficking.

Mitochondrial DNA Isolati Mitochondrial DNA Isolati Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Androgen Receptor (Ab 650 Cell Meter™ JC 10 Mitoc Cell Meter™ JC 10 Mitoc Cell Meter™ Mitochondri ATP synthase H+ transport Creatine kinase (mitochon

Related Pathways

paperclip

#29620509   // Save this To Up

Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.

Objective To screen the transient and stable cell lines with high production of Nectin-like 4 (Necl-4) protein. Methods First, cDNA sequences encoding the extracellular domain of Necls were cloned into the modified vector pAPtag at the N terminus of alkaline phosphatase (AP) for fusion expression. Next, 293ET cells stably expressed Necls-AP fusion protein and secreted it into the culture medium which were detected by the AP activity assay and Western blot analysis. Then, by adding N-glycosylation processing inhibitor kifunensine into the medium, complex glycan was inhibited to generate. The residual glycan of purified protein was removed by endoglycosidase H. Finally, AP protein was removed by using human rhinovirus protease and size exclusion chromatography. The concentration of purified Necl-4 protein was monitored by measuring the absorbance at 280 nm and analyzed by SDS-PAGE. Result The transient and stable cell lines with high production of Necl-4 protein were screened by the color reaction with the AP-tag in the recombinant vector. The soluble and active form of purified Necl-4 protein was obtained after deglycosylation of native N-glycan protein with an expression level of 4 mg/L culture and purity of 95%. Conclusions By using modified AP mammalian protein expression system, we can easily screen the high productive stable cell lines by using AP activity assay. By adding mannosidase inhibitor kifunensine into the medium and cutting purified protein by using endoglycosidase H, we can obtain deglycosylated Necl-4 protein in milligram quantities. Our method might throw a light on the expression and purification of glycoprotein for structural and functional studies.

2888 related Products with: Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line.

Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Macrophage Colony Stimula Macrophage Colony Stimula Recombinant Mouse Interle Recombinant Mouse Interle Recombinant Mouse Interle Recombinant Mouse Interle Recombinant Mouse Interle Recombinant Mouse Interle Recombinant Mouse Interle Recombinant Mouse Interle

Related Pathways

paperclip

#29615728   // Save this To Up

Causal Inference in the Perception of Verticality.

The perceptual upright is thought to be constructed by the central nervous system (CNS) as a vector sum; by combining estimates on the upright provided by the visual system and the body's inertial sensors with prior knowledge that upright is usually above the head. Recent findings furthermore show that the weighting of the respective sensory signals is proportional to their reliability, consistent with a Bayesian interpretation of a vector sum (Forced Fusion, FF). However, violations of FF have also been reported, suggesting that the CNS may rely on a single sensory system (Cue Capture, CC), or choose to process sensory signals based on inferred signal causality (Causal Inference, CI). We developed a novel alternative-reality system to manipulate visual and physical tilt independently. We tasked participants (n = 36) to indicate the perceived upright for various (in-)congruent combinations of visual-inertial stimuli, and compared models based on their agreement with the data. The results favor the CI model over FF, although this effect became unambiguous only for large discrepancies (±60°). We conclude that the notion of a vector sum does not provide a comprehensive explanation of the perception of the upright, and that CI offers a better alternative.

1392 related Products with: Causal Inference in the Perception of Verticality.

Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss MultiGene Gradient therm Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se

Related Pathways

  •  
  • No related Items
paperclip

#29607500   // Save this To Up

Discrimination of nitrogen fertilizer levels of tea plant (Camellia sinensis) based on hyperspectral imaging.

Nitrogen (N) fertilizer plays an important role in tea plantation management, with significant impacts on the photosynthetic capacity, productivity and nutrition status of tea plants. The present study aimed to establish a method for the discrimination of N fertilizer levels using hyperspectral imaging technique.

1828 related Products with: Discrimination of nitrogen fertilizer levels of tea plant (Camellia sinensis) based on hyperspectral imaging.

Ofloxacin CAS Number [824 Plant DNA Preparation Kit Bcl-2 Oncoprotein; Clone Bcl-2 Oncoprotein; Clone c-erbB-2 Oncoprotein c-erbB-2 Oncoprotein c-erbB-3 Oncoprotein; Cl c-erbB-3 Oncoprotein; Cl c-erbB-2 Oncoprotein; Cl c-erbB-2 Oncoprotein; Cl c-erbB-2 Oncoprotein; Cl c-erbB-2 Oncoprotein; Cl

Related Pathways

paperclip

#29599946   // Save this To Up

Organic Boundary Location Based on Color-Texture of Visual Perception in Wireless Capsule Endoscopy Video.

This paper addresses the problem of automatically locating the boundary between the stomach and the small intestine (the pylorus) in wireless capsule endoscopy (WCE) video. For efficient image segmentation, the color-saliency region detection (CSD) method is developed for obtaining the potentially valid region of the frame (VROF). To improve the accuracy of locating the pylorus, we design the model. On the one hand, the color-texture fusion feature of visual perception (CTVP) is constructed by grey level cooccurrence matrix (GLCM) feature from the maximum moments of the phase congruency covariance and hue-saturation histogram feature in HSI color space. On the other hand, support vector machine (SVM) classifier with the CTVP feature is utilized to locate the pylorus. The experimental results on 30 real WCE videos demonstrate that the proposed location method outperforms the related valuable techniques.

1227 related Products with: Organic Boundary Location Based on Color-Texture of Visual Perception in Wireless Capsule Endoscopy Video.

Inorganic Phosphorous, Co Rabbit Anti-FGF3 Oncogene Color inserts: Assorted Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Bcl-2 Oncoprotein; Clone Bcl-2 Oncoprotein; Clone c-erbB-2 Oncoprotein c-erbB-2 Oncoprotein c-erbB-3 Oncoprotein; Cl c-erbB-3 Oncoprotein; Cl c-erbB-2 Oncoprotein; Cl

Related Pathways

paperclip

#29599838   // Save this To Up

Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice.

The emergence of drug-resistant tuberculosis (TB) and HIV-TB co-infection fuels an urgent need to develop novel therapeutic approaches, including therapeutic vaccines. Therapeutic vaccines have been proven to be a good strategy by inducing antigen specific immune responses against TB infection. In the present study, a recombinant plasmid based on lentiviral vector expressing fusion antigen Ag85B-Rv3425 (A3), and was constructed the immunogenicity and treatment effects in TB mice were assessed. The results showed that A3 delivered by the plasmid could be expressed appropriately and induced higher production of tumor necrosis factor-α and interleukin-2 compared with A3 recombinant protein in mice. Moreover, the recombinant plasmid expressing A3 confered resistance to acute TB infection in mice, characterized by a reduction in the bacterial load in the lungs and spleen, as well as attenuated TB lesions in lung tissues. These results implicated that the recombinant plasmid based on lentiviral vector expressing A3 is a potent and promising therapeutic agent to treat acute TB infection.

2275 related Products with: Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice.

Recombinant Hemagglutinin Toxoplasma gondii GRA8, r FIV Core Ag, recombinant HIV 1 intergase antigen. Macrophage Colony Stimula Macrophage Colony Stimula anti H inh human blood an H. Pylori antigen test ca Malaria pan antigen test, Malaria pf antigen test, Malaria pf pv antigen tes Recombinant M. tuberculos

Related Pathways