Search results for: 280 nm Filter, AC_5100
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The nature of the light absorption and emission transitions of 4-hydroxybenzophenone in different solvents. A combined computational and experimental study.The photophysics and photochemistry of 4-hydroxybenzophenone (4HOBP) are interesting because they can give some insight into the behavior of humic material. Here we show that 4HOBP has a number of fluorescence peaks: (i) an intense one at excitation/emission wavelengths Ex/Em ∼ 200-230/280-370 nm, likely due to an excitation transition from Sto Sor S, followed by S→ Sin emission (Sdenotes the singlet states of 4HOBP); (ii) a minor peak at Ex/Em ∼ 270-300/320-360 nm (S→ Sin absorption and S→ Sin emission), and (iii) very interesting signals in the typical emission region of humic substances, most notably at Ex/Em ∼ 200-220/400-500 nm and Ex/Em ∼ 260-280/400-470 nm (in both cases the emission corresponded to an S→ Stransition). The peak (i) (Ex/Em ∼ 200-230/280-370 nm) is quite intense at low 4HOBP concentration values, but it undergoes an effective inner-filter phenomenon. Remarkably, 4HOBP shows fluorescence peaks that arise from S→ Stransitions and that do not follow Kasha's rule. Fluorescence is observed in aprotic or poorly protic solvents, and to a lesser extent in aqueous solution. The excited states of 4HOBP, and most notably 4HOBP-S, are much stronger acids than 4HOBP-S. Therefore, excited 4HOBP is quickly deprotonated to 4OBP-Sin ∼neutral solution, with a considerable loss of the fluorescence properties. Higher fluorescence intensity can be observed under acidic conditions, where excited-state deprotonation is less effective, and in basic solution where the dissociated 4OBP-Sform prevails as the ground state. The excited states of 4OBPare formed directly upon radiation absorption, and being weak bases they do not undergo important acid-base equilibria. Therefore, they can undergo radiational deactivation to produce significant fluorescence emission.
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Synchrotron X-ray phase contrast imaging of leaf venation in soybean (Glycine max) after exclusion of solar UV (280-400 nm) radiation.The hydraulic efficiency of a leaf depends on its vascular structure as this is responsible for transport activities. To investigate the effect of exclusion of UVAB and UVB radiation from the solar spectrum on the micro-structure of leaves of soybean (Glycine max, variety JS-335), a field experiment was conducted using synchrotron-based phase contrast imaging (PCI). Plants were grown in specially designed UV exclusion chambers, and wrapped with filters that excluded UVB (280-315 nm) or UVAB (280-400 nm), or transmitted all the ambient solar UV (280-400 nm) radiation (filter control). Qualitative observation of high-resolution X-ray PCI images obtained at 10 keV has shown the differences in major and minor vein structures of the leaves. The mid-rib width of the middle leaflet of third trifoliate leaves, for all treatments, were obtained using quantitative image analysis. The width of the mid-rib of the middle leaflet of third trifoliate leaves of UVB excluded plants was found to be more compared to leaves of filter control plants, which are exposed to ambient UV. The mid-rib or the main conducting vein transports water and sugars to the whole plant; therefore, mid-rib enhancement by the exclusion of solar UV radiation possibly implies enhancement in the leaf area which in turn causes an increased rate of photosynthesis.
2759 related Products with: Synchrotron X-ray phase contrast imaging of leaf venation in soybean (Glycine max) after exclusion of solar UV (280-400 nm) radiation.Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Inorganic Phosphorus, UV, Trypsin EDTA (1 250) UV i MAX & SMAD3 Protein Prote MAX & MSH2 Protein Protei MAPK14 & MAX Protein Prot Goat Anti-Human TUSC4 NPR Proteins and Antibodies H Ofloxacin CAS Number [824 Pressure Injection Cell, Pressure Injection Cell w
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Disentangling the effects of solar radiation, wrack macroalgae and beach macrofauna on associated bacterial assemblages.Wrack detritus plays a significant role in shaping community dynamics and food-webs on sandy beaches. Macroalgae is the most abundant beach wrack, and it is broken down by the combination of environmental processes, macrofauna grazing, and microbial degradation before returning to the sea as nutrients. The role of solar radiation, algal species and beach macrofauna as ecological drivers for bacterial assemblages associated to wrack was investigated by experimental manipulation of Laminaria ochroleuca and Sargassum muticum. We examined the effects of changes in solar radiation on wrack-associated bacterial assemblages by using cut-off filters: PAR + UVA + UVB (280-700 nm; PAB), PAR + UVA (320-700 nm; PA), PAR (400-700 nm; P), and a control with no filter (C). Results showed that moderate changes in UVR are capable to promote substantial differences on bacterial assemblages so that wrack patches exposed to full sunlight treatments (C and PAB) showed more similar assemblages among them than compared to patches exposed to treatments that blocked part of the solar radiation (P and PA). Our findings also suggested that specific algal nutrient quality-related variables (i.e. nitrogen, C:N ratio and phlorotannins) are main determinants of bacterial dynamics on wrack deposits. We showed a positive relationship between beach macrofauna, especially the most abundant and active wrack-users, the amphipod Talitrus saltator and the coleopteran Phaleria cadaverina, and both bacterial abundance and richness. Moderate variations in natural solar radiation and shifts in the algal species entering beach ecosystems can modify the role of wrack in the energy-flow of nearshore environments with unknown ecological implications for coastal ecosystems.
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Evaluation of Ag nanoparticle coated air filter against aerosolized virus: Anti-viral efficiency with dust loading.In this study, the effect of dust loading on the anti-viral ability of an anti-viral air filter was investigated. Silver nanoparticles approximately 11 nm in diameter were synthesized via a spark discharge generation system and were used as anti-viral agents coated onto a medium air filter. The pressure drop, filtration efficiency, and anti-viral ability of the filter against aerosolized bacteriophage MS2 virus particles were tested with dust loading. The filtration efficiency and pressure drop increased with dust loading, while the anti-viral ability decreased. Theoretical analysis of anti-viral ability with dust loading was carried out using a mathematical model based on that presented by Joe et al. (J. Hazard. Mater.; 280: 356-363, 2014). Our model can be used to compare anti-viral abilities of various anti-viral agents, determine appropriate coating areal density of anti-viral agent on a filter, and predict the life cycle of an anti-viral filter.
2167 related Products with: Evaluation of Ag nanoparticle coated air filter against aerosolized virus: Anti-viral efficiency with dust loading.Viral antibodies, anti-R MOUSE ANTI CANINE DISTEMP Recombinant Viral Antige MOUSE ANTI BOVINE ROTAVIR Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon
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Simultaneous Analysis of Tertiary Butylhydroquinone and 2-tert-Butyl-1,4-benzoquinone in Edible Oils by Normal-Phase High-Performance Liquid Chromatography.During the process of antioxidation of tertiary butylhydroquinone (TBHQ) in oil and fat systems, 2-tert-butyl-1,4-benzoquinone (TQ) can be formed. The toxicity of TQ was much more than that of TBHQ. In the work, a normal-phase high-performance liquid chromatography (NP-HPLC) method for the accurate and simultaneous detection of TBHQ and TQ in edible oils was investigated. A C18 column was used to separate TBHQ and TQ, and the gradient elution solutions consisted of n-hexane containing 5% ethyl acetate and n-hexane containing 5% isopropanol. The ultraviolet (UV) detector was set at dual wavelength mode (280 nm for TBHQ and 310 nm for TQ). The column temperature was 30 °C. Before the NP-HPLC analysis, TBHQ and TQ were first extracted by methanol, subjected to vortex treatment, and then filtered through a 0.45 μm membrane filter. Results showed that linear ranges of TBHQ and TQ were both within 0.10-500.00 μg/mL (R(2) > 0.9999). The limit of detection (LOD) and limit of quantification (LOQ) of TBHQ and TQ were below 0.30 and 0.91 μg/mL and below 0.10 and 0.30 μg/mL, respectively. The recoveries of TBHQ and TQ were 98.92-102.34 and 96.28-100.58% for soybean oil and 96.11-99.42 and 98.83-99.24% for lard, respectively. These results showed that NP-HPLC can be successfully used to analyze simultaneously TBHQ and TQ in the oils and fats.
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Use of FTA cards for the storage of breast carcinoma nucleic acid on fine-needle aspiration samples.The preservation and storage of nucleic acids is important for DNA molecular techniques. The material obtained by fine-needle aspiration (FNA) is often scanty and can not be wasted. FTA cards are filter papers that immobilize and stabilize nucleic acids and can be stored at room temperature. The current study evaluated whether nucleic acids of breast carcinoma cells, obtained by FNA in a clinical setting, may be collected, stored, and preserved on FTA cards.
1329 related Products with: Use of FTA cards for the storage of breast carcinoma nucleic acid on fine-needle aspiration samples.Breast invasive ductal ca Cancer Samples: Breast C Anti AGE 3 Monoclonal Ant GELRED NUCLEIC ACID STAIN GELRED NUCLEIC ACID STAIN GELRED NUCLEIC ACID GEL S GELRED NUCLEIC ACID GEL S GELRED NUCLEIC ACID STAIN GelRed Nucleic Acid Stain GELRED NUCLEIC ACID GEL S GELREDTM NUCLEIC ACID GEL GelRed Nucleic Acid Stain
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Total protein concentration and diagnostic test results for gray wolf (Canis lupus) serum using Nobuto filter paper strips.Nobuto filter paper strips are widely used for storing blood-serum samples, but the recovery of proteins from these strips following rehydration is unknown. Poor recovery of proteins could reduce the concentration of antibodies and antigens and reduce the sensitivity of diagnostic assays. We compared the protein concentration, and its association with test sensitivity, of eluted Nobuto strip samples with paired sera. We collected and froze serum from five gray wolves (Canis lupus) for 8 mo. When thawed, we used a spectrophotometer (absorbance 280 nm) to determine the serum protein concentration for paired sera and Nobuto eluates for each animal in 2-fold serial dilutions. Total protein concentration was similar for both sample storage methods (Nobuto eluates and control sera), except for the undiluted samples in which Nobuto eluates had higher total protein concentrations. Both sample storage methods appear to produce similar results using the SNAP® 4Dx® Test to detect antibodies against pathogens causing Lyme disease, anaplasmosis, and ehrlichiosis as well as antigen for canine heartworm disease.
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Modelling of diffraction grating based optical filters for fluorescence detection of biomolecules.The detection of biomolecules based on fluorescence measurements is a powerful diagnostic tool for the acquisition of genetic, proteomic and cellular information. One key performance limiting factor remains the integrated optical filter, which is designed to reject strong excitation light while transmitting weak emission (fluorescent) light to the photodetector. Conventional filters have several disadvantages. For instance absorbing filters, like those made from amorphous silicon carbide, exhibit low rejection ratios, especially in the case of small Stokes' shift fluorophores (e.g. green fluorescent protein GFP with λ exc = 480 nm and λ em = 510 nm), whereas interference filters comprising many layers require complex fabrication. This paper describes an alternative solution based on dielectric diffraction gratings. These filters are not only highly efficient but require a smaller number of manufacturing steps. Using FEM-based optical modelling as a design optimization tool, three filtering concepts are explored: (i) a diffraction grating fabricated on the surface of an absorbing filter, (ii) a diffraction grating embedded in a host material with a low refractive index, and (iii) a combination of an embedded grating and an absorbing filter. Both concepts involving an embedded grating show high rejection ratios (over 100,000) for the case of GFP, but also high sensitivity to manufacturing errors and variations in the incident angle of the excitation light. Despite this, simulations show that a 60 times improvement in the rejection ratio relative to a conventional flat absorbing filter can be obtained using an optimized embedded diffraction grating fabricated on top of an absorbing filter.
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Isotope ⁸⁷Rb Faraday anomalous dispersion optical filter at 420 nm.We demonstrate a Faraday anomalous dispersion optical filter (FADOF) operating on 5S(1/2)-6P(3/2) transition at 420 nm with a 5 cm long cell of 96.5% enriched 87Rb and measure the transmission as a function of the magnetic field and temperature. The isotope 87Rb FADOF achieves a peak transmission of 98% with a bandwidth of 2.5 GHz when the temperature of the isotope 87Rb cell is 280°C and the magnetic field is 500 G. The corresponding equivalent noise bandwidth is 5.9 GHz. The isotope 87Rb FADOF is applied to submarine communications, is used as the laser frequency stabilization for some atomic lines, and is also used as the pump laser in four-level Rb active optical clock.
Sterile filtered goat se Sterile filtered goat se Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser ATF3 Bone Morphogenetic Protei Growth Differentiation Fa Human Beta-cell Attractin Caspase-12 Inhibitor Z-AT Caspase-12 Inhibitor Z-AT
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3D imaging of enzymes working in situ.Today, development of slowly digestible food with positive health impact and production of biofuels is a matter of intense research. The latter is achieved via enzymatic hydrolysis of starch or biomass such as lignocellulose. Free label imaging, using UV autofluorescence, provides a great tool to follow one single enzyme when acting on a non-UV-fluorescent substrate. In this article, we report synchrotron DUV fluorescence in 3-dimensional imaging to visualize in situ the diffusion of enzymes on solid substrate. The degradation pathway of single starch granules by two amylases optimized for biofuel production and industrial starch hydrolysis was followed by tryptophan autofluorescence (excitation at 280 nm, emission filter at 350 nm). The new setup has been specially designed and developed for a 3D representation of the enzyme-substrate interaction during hydrolysis. Thus, this tool is particularly effective for improving knowledge and understanding of enzymatic hydrolysis of solid substrates such as starch and lignocellulosic biomass. It could open up the way to new routes in the field of green chemistry and sustainable development, that is, in biotechnology, biorefining, or biofuels.
Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Cultrex96 Well 3D BME Cel Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti PolyTek HRP Anti-Rabbit PolyTek HRP Anti-Mouse P Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s
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